The volume of 0₂ gas produced from the reaction of BCG suspension with H₂0₂ was determined manometrically.
1. INAH, N-isonicotinyl-N'-isopropyl hydrazine and N-isonicotinyl-N'-glucosyl hydrazine inhibit the katalase of BCG in the order.
2. Katalase of BCG resistant to INAH is inhibited by INAH as same as in case of sensitive acilli.

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Golden hamsters were inoculated with 5-30 mg of BCG or 0.1 mg of human type of tubercle bacilli, H₃₇Rv, and autopsied for macroscopical, histological as well as for bacteriological examinations. All animals remained always negative in the tuberculin test even with 1: 10 dilution of old tuberculin.

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Reports on Candidiasis, a disease hitherto seldom noted, have increased in number following the clinical application of various antibiotics recently. Various theories have been advanced on the relation between antibiotics and Candida, some assuming that the former has a direct growth promoting effect on the latter and the other that the shortage of vitamin B caused by the former constitutes an indirect cause of the growth of the latter. None of these ideas are based on any established experimental findings and opinions differ on the mechanism by which Candidiasis is brought about. It is easily conceivable, however, that the Candida may appear and proliferate during the treatment with antibiotics. The present author, viewing the problem from such angle, attempted the isolation of Candida from the sputum and feces of tuberculosis patients and normal persons, with the following results:

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Comparative studies were made on the cultural and immunobiological characteristics between Scandinavian and Japanese BCG strains.
Each of them grew very nice on the potato-media and their growths were good on the bilepotato medium even from the first transplantation. Their growths were also good on the Sauton medium, but the pellicles were rather thick compared with that of Japanese strain. The growth speed on this medium was slightly more rapid in Swedish and Norwegian strains than that of Danish and Japanese. but the viable units per mg of pellicle were the greatest in Japanese, strain.
The bacillary size was compared on the smears prepared from Sauton-potato cultures and it was found that the three strains, Danish, Swedish and Japanese had almost the same average size with similar distribution curves, whereas, Norwegian strain only had rather big size like French strain reported in the foregoing paper.
Virulence test as well as challenge test on guinea pigs did not show any difference among those four strains except the difference due to the different amount of viable units inoculated.
From these results it is considered that for the maintenance of the original characteristics of BCG strain, it is best at present to maintain it only by the successive transplantation on bilepotato medium.

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The author has previously reported, in Part I, that a small amount (0.004 mg) of liquid BCG vaccine and its dead BCG vaccine can be used for diagnostic purpose in child-tuberculosis, and that their sensitivity is even stronger than that of the old tuberculin reaction (0.05 mg)
This time, the inoculation was made intracutaneously into the forearm of a child with 0.004 mg of dry BCG vaccine which had been treated by, boiling and Koch's phenomenon produced was examined.

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It has been well known that bacterial agglutination will take place by physical or chemical influence in the absence of any specific agglutinin. Reported in the following are the findings of the agglutination of tubercle bacilli by dihydrostreptomycin (DSM).

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The present study was carried out on about 810 cases of pulmonary tuberculosis from ten national sanatoria in Tohoku districts. The cases had been treated with pneumothorax for a period over six months and observed more than three years after abandonment of the treatment. Among them 766 cases (890 sides) were followed up and analyzed. The mean duration of the follow-up after abandonment of pneumothorax was about 7.5 years, and the results were as follows: at work 43.3, under treatment 14.9 and dead 41.8 per cent. In the 291 cases whose response to pneumothorax had been entirely satisfactory, the cumulative mortality due to tuberculosis alone was 10.5 per cent after five years.

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Four types of gases, air, carbon dioxide, oxygen and nitrogen were injected independently into the peritoneal cavities of rabbits and tuberculous human cases, after which details of the changes produced in the composition of injected gases were examined systematically. First of all, no marked differences between human and rabbit were noticed throughout all the gases injected in the mariner of changing the composition on each time of the examinations made at 1 hour, 6 hours, 12 hours and 24 hours after injection.

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