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Volume 43, Issue 4
Displaying 1-6 of 6 articles from this issue
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Michio TSUKAMURA, Shoji MIZUNO, Haruo TOYAMA, Sumio TSUKAMURA1968 Volume 43 Issue 4 Pages 119-127
Published: 1968
Released on J-STAGE: May 24, 2011
JOURNAL FREE ACCESSAs a member of the Group III Cooperative Study organized by Dr. E. H. Runyon, the present writers received 39 strains labelled as M. avium, M. terrae, M. gastri, pathogenic group III nonphotochromogens M. intracellulare (Runyon, E. H.. Amer. Rev. Resp. Dis., 95: 861-865, 1967), M. xenopei, M. scrofulaceum, M. marianum, and a few soil and pig isolates. We studied on these strains together with our M. terrae strains (Tsukamura's M. terrae (nonchromogenicum))(Tsukamura, M.: Med. & Biol., 71: 110-113, 1965; 72: 292-295, 1966) according to the schedule previously described (Tsukamura, M. et al.: Kekkaku, 41: 395-399, 1966; Tsukamura, M.: J. Gen. Microbiol., 45: 253-273, 1966).View full abstractDownload PDF (963K) -
Akira MURATA1968 Volume 43 Issue 4 Pages 129-134
Published: 1968
Released on J-STAGE: May 24, 2011
JOURNAL FREE ACCESSIn the Part III, the problem of virulence of INH resistant bacilli is discussed biochemically according to the results of oxydation-reduction dye test, catalase-reaction and peroxydasereaction.
For this purpose, 73 M. tuberculosis strains obtained from patient's sputa when their exacerbation were detected rontgenologically, were collected from all national sanatoria from May thru September, 1966. The investigation revealed that:
1) Dye-test failed to prove that the virulence was reduce d because the strain was high INH resistant.
2) In catalase-and peroxydase-reaction, the number of negative cases was increased as the degree of INH resistance was raised.
3) INH resistance test on 73 s t rains obtained from patients with radiographic exacerbation revealed a resistance over 5 mcg in 8.2%. A high INH resistant strain does not necessarily imply a harmless strain.
In view of the above results, there is little doubt that some biological change must take place in INH resistant strains, but it was difficult to establish a relationship between their virulence, especially to human body, and resistance to INH.View full abstractDownload PDF (664K) -
Masatoshi TAMURA, Akira YAMAZAKI1968 Volume 43 Issue 4 Pages 135-139
Published: 1968
Released on J-STAGE: May 24, 2011
JOURNAL FREE ACCESSStudies were made about the effect of the amount of bacilli for inoculation and the period of incubation on the minimal inhibitory concentration of 1314 TH to the growth of Myc. tuberculosis. One % Ogawa's media and Kirchner's semi-liquid agar media with albumin in 10%, were used. The concentrations of 1314 TH of the media were so prepared as to become 0, 1, 5, 10, 20, 50, 100 and 200 mcg/ml respectively. These media were mixed thoroughly, dispensed into test tubes in 5 ml quantity, and used after being stored in an incubator at 37°C for 24 hours in order to confirm the sterility. In consideration of our previous experience, the inoculations were made immediately after the preparation of the media. Three strains were used for this experiment, that is, H37Rv, Aoyama-B and a strain isolated from the sputum of a pulmonary tuberculosis patient without any history of previous treatment. These strains were planted on 1% Ogawa's egg media, and 2 week old cultures were always used for the experiment. Bacillary suspensions were prepared from these cultures, and by using electric photometer the amounts for inoculation were so prepared as to become 10-1, 10-2, 10-3 and 10-4 mg in 0.1 ml. After the inoculation, the media were incubated at 37°C fo r 6weeks and submitted to the weekly observation of the growth of colonies. The following results were obtained. The minimal inhibitory conc e ntration of 1314 TH to the growth of Myc. tuberculosis tends to become higher with the increasing amount of inoculum. In either medium the effect of incubation peri o d on the minimal inhibitory concentration was very marked, the latter increasing obviously with prolonged incubation period. Such effect was more obvious in 1% Ogawa's medium than in Kirchner's medium. When in Ogawa's medium 10-3 mg of inoculum was employe d, the minimal inhibitory concentrations of 1314 TH were 5 mcg/ml, 20 mcg/ml and 50 mcg/ml respectively in 2 week-, 3 weekand 4-5 week-old cultures. The corresponding values in Kirchner's medium were 5 mcg/m/and 10 mcg/ml in 2 week- and 3 week-old cultures. The marked rise in the minimal inhibito r y concentration observed in the case of prolonged incubation period is probably due to the decrease of drug potency by the effect of a high temperature of the incubator.View full abstractDownload PDF (596K) -
(9th Series of Cooperative Study, Part B-2)[in Japanese]1968 Volume 43 Issue 4 Pages 141-145
Published: 1968
Released on J-STAGE: May 24, 2011
JOURNAL FREE ACCESSOn the occasion of 9-B Series of the CSUCT Study, the relationship between incidence of complaints or clinical symptoms on the one hand and Cornell Medical Index (CMI) of the subjects on the other was investigated.
In the investigation, Form a (Fig.1) was filled up by physicians in charge and Form b (Fig.2) by patients themselves. As for the group A patients (84 cases), both Form a and b were utilized, while as for the group B patients (107 cases), only Form a was filled up as shown in Fig.3.
The frequency of I, II, III and IV levels of CMI in A and B group was indicated in Table
1. High coincidence between the two groups was confirmed.
The incidence of complaints before the start of the trial classified by CMI is demonstrated in Fig.4 and the incidence one month after the start of treatment in Fig.5. There is a definite trend that the number of complaints or symptoms increased in the order of I, II, III and IV levels.
The incidence of symptoms complained by the patients (Form a) and those confirmed by the physicians(Form b)are compared in Figs.6, 7 and 8. A remarkable discrepancy is revealed in subjects on II, III and IV levels, especially during chemotherapy.
In Figs.9 and 10, the frequency and duration of in d ividual symptom classified by CMI is indicated.
In conclusion, random allocation of subjects or stratification of them by CMI is a necessary prerequisite for toxicity study, as marked difference was observed in the incidence rate of complaints and symptoms among the patients of I, II, III and IV levels of CMI during drug therapy.View full abstractDownload PDF (519K) -
Michio TSUKAMURA, Shoji MIZUNO, Haruo TOYAMA, Yasunobu ITASAKA, Hiroka ...1968 Volume 43 Issue 4 Pages 147-154
Published: 1968
Released on J-STAGE: May 24, 2011
JOURNAL FREE ACCESSA case of simultaneous infection with Mycobacterium intracellulare and Mycobacterium fortuitum (subsp. abscessus) is described. The patient is born in April 1927 in Aichi-Prefecture and is a married woman. She had cough and sputa in March 1966 and entered this hospital under diagnosis of pulmonary tuberculosis with a cavity in the left upper lobe. She was suspected of infection with atypical mycobacteria by growth of her bacilli on salicylate medium (Ogawa egg medium containing 0.5mg/m/ sodium salicylate (9)) and growth on all media containing antituberculous agents inspite of absence of previous treatment with antituberculous agents. By examination of the organism excreted to sputum, the organism was identified as M. f ortuitum subsp. abscessus. After administration of antituberculous agents, which wer e considered to be ineffective according to drug resistance tests, the cavity of the left upper lobe was markedly decreased and appeared as if disappeared on a X-ray figure. In February 1967, she received resection of the left upper lobe. Result of cultivation of cavity wall and a part of lung with cavity showed growth of 36 colonies after 5 months. This organism was identified as M. intracellulare, but a varity showing very slow growth. It was suggested that this slow growth may be due to lack of ability to utilize many nitrogen compounds. Course of excretion of acid-fast organism, chemotherapy ap p licated, result of drug resistance tests, course of change of X-ray figure, tuberculin reaction, biologic and biochemical characters of the organisms isolated, and virulence for mice are shown in tables and figure. The patient is excreting M. f ortuitum even after the resection of the left upper lob e and is considered that the organism is infected at a site other than the cavity of the left upper lobe. From the cavity, only M. intracellulare was found. It is noteworthy that even an organism b e l onging to a rapid grower shows very slow growth at primary isolation from sputum, but it shows rapid growth (growth at 3 days on egg media)at secondary cultures. The M. intracellulare isolated in this case showed very slow growth, a little slower than M. tuberculosis.View full abstractDownload PDF (799K) -
1968 Volume 43 Issue 4 Pages 160
Published: 1968
Released on J-STAGE: May 24, 2011
JOURNAL FREE ACCESSDownload PDF (68K)
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