It is said that the reticulo-endothelial system produces antibodies, and therefore, in an attempt to block the reticulo-endothelial system using rabbits, 5.0 ml of 1% tripanblau solution was injected intravenously every day for 10 days and then 10 mg of tubercle bacilli (Frankfurt strain) or BCG was inoculated subcutaneously. After that the tuberculin reaction, the phagocytosis of leucocytes and the specific hemobacteriostatic power against tubercle bacilli were tested. Following results were obtained:
1. The phagocytosis of leucocytes in healthy rabbits with negative ROmer's reaction was weak, and the specific hemobacteriostatic power was varied and weak.
2. The tuberculin reaction in rabbits after inoculation of both BCG and tubercle bacilli (Frankfurt strain) became positive in 2 weeks, and the phagocytosis of leucocytes and the specific hemobacteriostatic power increased as time went on.
3. The tuberculin reaction in the blocked rabbits appeared a week later than that of the non-blocked rabbits, and their phagocytosis of leucocytes and the specific hemobacteriostatic power were weaker than those of the non-blocked rabbits.
4. The reticulo-endotherial system is related to the production of antibodies..

View full abstract

Regional lymphnode of rabbits infected subcutaneously with bovine tubercle bacilli (dose 5 mg) were observed histologically and cytologically. Findings of the examination were as follows:
1. Intense exudation of polymorphonuclear leucocytes in lymphatic sinus was found in early stage, but it seems to be non-specific, because this change was not transformed to the tubercle formation or caseation.
2. Five days after inoculation we observed the epithelioidcell tubercle, which consists merely of some epithelioidcells, and has no other cells in inside. These tubercles are formed mostly in the peripheral part of the cortical parenchyma, especially near the medullar sinus. Infiltration of polymorphonuclear leucocytes in tubercles was found after tubercles were enlarged in later stage.
3. Reticulum cells of cortical parenchyma increase and swell and gradually show inclination to grouping. These concentrated reticulum cell groups show epithelioidcell-tubercle-like picture.
4. Nucreus of epithelioidcell has reticulum cellular character, and also there was recognized a transitional form between these two cells.
5. Some polymprphonuclear leucocytes which were observed in early stage show the increase of basophility of protoplasma and swelling of nucleus, then they become difficult to distinguish from rnonocytes.
The results of the present study indicate that first tubercles are formed in the peripheral part of cortical parenchyma near the medullar sinus, and that the formation of epithelioidcell tubercle has close relation to the increase of intraparenchymal reticulum cells.

View full abstract

1. On lobular and segmental broncho-tuberculosis, the presence of ulceration, tubercle, infiltration, fibrosis, stricture and that of delatation were examined.
The lesions most frequently encountered were infiltration and fibrosis which was followed by ulceration and tubercles in the order listed. The frequency of finding stricture was practically the same between the lobular and the segmental broncho-tuberculosis while the frequency of the appearance of delatation was mostly in the former.

View full abstract

When INAH was given for 6 months consecutively, the rate of cases whose sputum cultures became negative was higher than when TB1 treatment was given for 6 months or the combined treatment for 6 months or SM treatment for 40 days. When the treatment was continued longer than 6 months the rate was higher than the one when the treatment was continued less than 6 months.

View full abstract

1. The relation between the inc3bation day and the decomposition product of 1-tyrosine by avian type tubercle bacilli
Tyrosol was reported in the preceding report as the splitting product of 1-tyrosine by avian type tubercle bacilli. For the purpose of studying the chemical changes of the tyrosol formation, Takeo strain was inoculated on Sauton's medium containing 0.1% 1-tyrosine for varyiTig periods of time. The results indicated that the decomposition of 1-tyrosine was slight for the first days, but almost completely decomposed within 10 days. Maximal tyrosol formation was observed on the 20th day. By the end of 40 days, p-hydroxyphenyl-acetic acid was obtained.
2. The decomposition of tyrosine derivatives by avian type tubercle bacilli Sauton's medium. containing 0.1% of tyramine, p-hydroxyphenyl-propionic acid or d-p-hydroxyphenyllactic acid in the place of l-tyrosine, was used in order to investigate the mechanism of tyrosol formation from 1-tyrosine by Takeo strain. Tyrosol was formed, in the experiment with tyramine, giving almost similar yield as from l-tyrosine. But in the case of the latter two acid substances, the formation of tyrosol was proved only by paperchromatography.

View full abstract

From unheated and heated culture filtrates of human tubercle bacilli and BCG grown on synthetic media, three different proteins and two different polysaccharides were fractionated according to their solubility and precipitability by alcohol precipitation at low temperature.
The fractions obtained from unheated filtrates were designated by Seibert as protein A, B and C and polysaccharides and II; and those obtained from heated filtrates were provisionally named by the present author as protein hA, hB and hC and polysaccharides hI and hII.

View full abstract

In our previous papers (1st and 2nd), an experimental method for making tuberculous cavity in rabbit's lung was reported. All the treated rabbit's lungs presented the formation of tuberculous cavities similar to those observed in the lungs of human beings. Reported in the following are the findings of experimental tuberculous cavity formation in the rabbit's lung by the similar method as stated in the first report, except that killed tubercle bacilli were used as the second antigen. Rabbits were sensitized by injection of heat-killed bovine tubercle bacilli (Miwa strain), paraffine oil, anhydrous lanolin, and homogenate of rabbit's lung as reported before.

View full abstract

Experimental method
Enzyme preparation: Cell suspension or acetone dry powder or extracted rough material of Takeo strain of avian tubercle bacilli was used as enzyme preparation. Paper chromatographic method was applied for the quantitative determination of glutamic acid derived from the reaction of a-ketoglutaric acid and a nurnber of amino acids with the above enzyme preparation for 2.5 to 3 hours at 37.5°C under anaerobic condition, pH ranging from 7.4 to 7.8.

View full abstract