Upon studying the relation between relapse (Schub) of tuberculosis patients and atmospheric phenomena, for convenience' sake, the relation existing between so-called relapse and atmospheric phenomena was studied and the following are the results obtained thereof.
1. I have succeeded in producing intestinal tuberculosis closely resembling that found in human cases by directly inoculating tubercle bacilli (typus humanus) into submucosa or subserosa of duodenum (Caput pancreatis) of guinea pig.
As noted in Report I, π can be easily and accurately dispensed into ampules in any minute quantities by our own method in which the lyophilizing process is utilized. The finished products thus obtained have an extremely high solubility and they can be readily put to practical use. In the present report the storage of lyophilized π will be dealt with.
Inhibition of exudation and quickening of the absorption of exudate have been accounted as characteristic tissue reactions of living body which followed the administration of streptomycin. While, on the other hand, depending on the stage of administration of streptomycin, atrophy of epithelioid cells, weakening of the encapsulation of caseous lesions or hyalin degeneration of tubercles may be observed. As to whether they are due to the strong influence on tubercle bacilli exerted by streptomycin or due to the changes produced in the reactivity of the tissue cell system of living body, there are many problems yet to be solved.
Influence of combined application of antituberculous agents on the resistance of in vivo tubercle bacilli was examined availing the strain obtained by the quantitative cultivation test made on various organs of the guinea pigs used in the evaluation of the therapeutic effect of combined application on the experimentally produced tuberculosis.
Yamamura et al reported on several types of adaptation observed while undertaking experiments with the suspensions of Mycobacterium tuberculosis var. avium (Takeo Strain). The mechanism of successive adaptation and relative chemical adaptation have been clarified. Following are the findings of the experiments made with mandelic acid adapted cells of M. tuberculosis var. aviurn. (Warburg's manometer and Beckman's spectrophotometer were used in the experiments.)
Immunogen prepared from killed tubercle bacilli was applied on tuberculosis patients with positive tuberculin reactions not to speak of strong positive reactors, on mild and moderate tuberculosis patients with negative tuberculin reaction and also on tuberculin negative, positive and strong positive normal individuals (Kekkaku: Vol.27, No.1).