Carotenoids have been known to be effective in preventing a variety of diseases such as cancers and cardiovascular disorders. Mammalian cells cannot synthesize carotenoids and rely on a dietary source for their intake. Here we report the establishment of mammalian cells synthesizing phytoene, a kind of carotenoid, by introduction of the
crtB gene, phytoene synthase gene, which was previously cloned from a bacterium,
Erwiuia uredovora. The phytoene-producing cells acquired a resistance against oxidative stress induced by Fe
3+/adenosine 5'-diphosphate. The data suggest the feasibility of a genetic chemoprevention for oxidative stress-related disorders by endogenous synthesis of phytoene in mammalian cells.
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