A simple method with the injection of horse serum to increase the number of mitotic cells in the kidney tissue of fishes is recommended as follows: (1) Inject 0.2g horse serum (per 10g body weight of the specimen) into the muscle; (2) inject again by the same way on the fourth day after the first injection; and (3) prepare chromosome slides as usual on the fifth day after the first injection.
The nature and origin of unusually long acrocentric marker chromosomes were investigated in relation to radioresistance with the application of the Q-banding method. In cells receiving 800rad, there were five types of unusually long acrocentric markers, different in structure. Evidence presented seems to suggest that all the strains with the LA markers are not always radioresistant.
The occurrence of Ivemark syndrome with poly-splenia in sibs is presented in this paper. This is the fifth example of this case so far reported. Prenatal detection was made in the second child at nine months of pregnancy by means of abdominal fetal ECG and fetography. The two infants dealt with were recognized as poly-splenia with cardiovascular and abdominal visceral abnormalities at necropsy.
The polydipsia and polyuria were found in aged male rats of the inbred Wistar/Tw strain. Water intake and urinary volume (ml/day/100g BW) were markedly greater in 17-month-old males than in 6-month-old males. The urinary concentrations of electrolytes and glucose were lower in aged polydiptic male rats than in young males. After a single injection of antidiuretic hormone (ADH), water intake of aged polydiptic rats was temporarily lowered to the level of young rats receiving no ADH injection. This suggests that polydipsia and polyuria were the consequences, at least in part, of the insufficiency of ADH secretion.