We reinvestigated the involvement of the nicotinic-acetylcholine receptor in catecholamine secretion from chick adrenal chromaffin cells. Nicotine (10
-8-10
-4M), as well as methacholine, caused a dose-dependent increase in the cytosolic concentration of Ca
2+ and catecholamine secretion from freshly isolated adrenal chromaffin cells. The effects of acetylcholine (10
-4M) were effectively inhibited by both hexamethonium (10
-4M) and atropine (10
-5M), and almost completely blocked by both antagonists. The results suggest the existence of functional nicotinic receptors, which mediate Ca
2+ mobilization and catecholamine secretion, in chromaffin cells of chick adrenal glands.
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