Proceedings of the Japan Academy, Series B
Online ISSN : 1349-2896
Print ISSN : 0386-2208
ISSN-L : 0386-2208
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Volume 79B, Issue 5
Displaying 1-3 of 3 articles from this issue
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Original Papers
  • The waveforms of VLF electromagnetic waves recorded at the time of the 1995 Hyogo-ken Nanbu earthquake
    Jun IZUTSU, Kazuo OIKE
    2003 Volume 79B Issue 5 Pages 125-130
    Published: 2003
    Released on J-STAGE: May 19, 2004
    DOIhttps://doi.org/10.2183/pjab.79B.125
    JOURNAL FREE ACCESS
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    The VLF waveforms of the electromagnetic waves recorded in videotape during a few hours before and after the Hyogo-ken Nanbu (Kobe) earthquake (M=7.3) have been examined. We classified the waveforms and investigated their origins. Comparison with the lightning data, provided by electric power companies, indicated that most of waveforms were explained as lightning origin. The waves clearly associated with the earthquake were only those recorded at the time of S wave arrival.

    (Communicated by Seiya UYEDA, M. J. A., May 12, 2003)
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  • Identification of promoter sequences using archaeal genomic sequences
    Naoki AMANO, Kuniaki TSUJI, Masashi SUZUKI
    2003 Volume 79B Issue 5 Pages 131-136
    Published: 2003
    Released on J-STAGE: May 19, 2004
    DOIhttps://doi.org/10.2183/pjab.79B.131
    JOURNAL FREE ACCESS
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    Using the genomic sequence of the hyperthermophilic archaeon Pyrococcus sp. OT3, 381 operons and 644 independently regulated genes have been theoretically identified on the basis of the presence or absence of transcription and translation signals upstream of open reading frames, and that of the triplet phasing for combining the purines and pyrimidines inside. Positioned upstream, regions of at shortest 50-60 bps appear to function as promoters for specifically regulating transcription of these 1, 025 units.

    (Communicated by Masanori OTSUKA, M. J. A., May 12, 2003)
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  • Fluorescent derivatization reagents for thiols bearing a new type of fluorophores: 7-fluoro-2, 1, 3-benzoselenadiazole-4-sulfonate and 7-fluoro-2, 1, 3-benzothiadiazole-4-sulfonate
    Chifuyu TORIUMI, Tomofumi SANTA, Kazuhiro IMAI
    2003 Volume 79B Issue 5 Pages 137-140
    Published: 2003
    Released on J-STAGE: May 19, 2004
    DOIhttps://doi.org/10.2183/pjab.79B.137
    JOURNAL FREE ACCESS
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    7-Fluoro-2, 1, 3-benzoselenadiazole-4-sulfonate (SBSeD-F) and 7-fluoro-2, 1, 3-benzothiadiazole-4-sulfonate (SBThD-F) were synthesized and proposed as the water-soluble fluorescent derivatization reagents for thiols bearing a new type of fluorophores (benzoselenadiazole and benzothiadiazole). The maximum excitation wavelengths of the derivatives of cysteine with SBSeD-F (340 nm) and SBThD-F (315 nm) were shorter than that with ammonium 7-fluoro-2, 1, 3-benzoxadiazole-4-sulfonate (SBD-F) (365 nm), respectively, while the maximum emission wavelength with SBSeD-F (542 nm) was longer than those with SBThD-F (517 nm) and SBD-F (514 nm). The time required for the quantitative derivatization of cysteine with SBSeD-F or SBThD-F was less than 24 h at pH 10.0 and 60°C, while the derivatization was achieved within 1 h with SBD-F. The appropriate derivatization condition was settled with 2 mM SBSeD-F or SBThD-F as 24 h reaction at 60°C in 0.1 M borate buffer (pH 10.0) in the presence of 0.5 mM EDTA.

    (Communicated by Masanori OTSUKA, M. J. A., May 12, 2003)
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