順天堂医学
Online ISSN : 2188-2134
Print ISSN : 0022-6769
ISSN-L : 0022-6769
19 巻, 1 号
選択された号の論文の20件中1~20を表示しています
目次
特集:呼吸器疾患診断最近の進歩
医学概論
原著
  • HIROMI KUBAGAWA, HIDEOKI OGAWA
    1973 年 19 巻 1 号 p. 79-83
    発行日: 1973/03/10
    公開日: 2014/11/22
    ジャーナル フリー
    This report describes a new method of disc electrophoresis for strongly basic proteins using arginine-acetate buffer at pH 9.0 as a tray buffer. The electrophoretic patterns of various basic proteins (i. e., HML, EWL, cytochrome C and histone) were studied and compared with acidic and alkaline conditions. As to lysozymes, the mixture of HML and EWL, clearly separated into two discs under the alkaline condition although the electrophoretic mobilities of both lysozymes under acidic condition were identical or not significantly different. Cytochrome C, which exhibited a main brown disc under acidic condition, divided into two discs with brown color due to ferric ion. In histone, 7 discs that were detected in acidic condition separated into at least 15 discs under the alkaline condition.
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