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Volume 37, Issue 4
Displaying 51-69 of 69 articles from this issue
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Osamu TAKEDA, Seiji TANAKA, Kazuo YAMASAKI, Hiroshi KOHDA, Yuzuru IWAN ...1989 Volume 37 Issue 4 Pages 1090-1091
Published: April 25, 1989
Released on J-STAGE: March 31, 2008
JOURNAL FREE ACCESSThirty-four extracts of crude drugs and medicinal plants have been screened for activity against Oncomelania nosophora, the intermediate host of the Japanese strain of Schistosoma japonicum. Strong molluscicidal activity was found in the MeOH extract of Anemarrhenae Rhizoma. Although timosaponin A-III, one of the main saponins of Anemarrhenae Rhizoma, showed very strong killing activity, markogenin-3-O-β-D-glucopyranosyl (1-2)-O-β-D-galactopyranoside having the same glycosidic linkage was found to be inactive.View full abstractDownload PDF (431K) -
Koichi MACHIDA, Keisuke OSAWA1989 Volume 37 Issue 4 Pages 1092-1094
Published: April 25, 1989
Released on J-STAGE: March 31, 2008
JOURNAL FREE ACCESSFrom the peels of Citrus hassaku HORT. ex TANAKA (Rutaceae), eight flavonoid aglycones were isolated and characterized as 4', 5, 6, 7, 8-pentamethoxyflavone (I), 3', 4', 5, 6, 7, 8-hexamethoxyflavone (II), 3, 3', 4', 5, 6, 7, 8-heptamethoxyflavone (III), 3', 4', 5, 6, 7-pentamethoxyflavone (IV), 4', 5, 7-trimethoxyflavone (V), 3, 3', 4, ', 5, 7, 8-hexamethoxyflavone (VI), 4', 5, 7, 8-tetramethoxyflavone (VII) and 3', 4', 5, 7, 8-pentamethoxyflavone (VIII), based on spectral and chemical data.View full abstractDownload PDF (515K) -
Kenji SHIMADA, Masaharu NAKAJIMA, Hiroyuki WAKABAYASHI, Susumu YAMATO1989 Volume 37 Issue 4 Pages 1095-1096
Published: April 25, 1989
Released on J-STAGE: March 31, 2008
JOURNAL FREE ACCESSA rapid and simple flow injection analysis for the determination of germanium (Ge) is described. Ge is reacted with phenylfluorone in a mixing coil (0.5 mm i.d.×300 cm) at 40°C, and the developed color is measured at 508 nm. The calibration graph is linear over the concentration range of 0.04-10 μg ml-1 (2-500 ng). Interference by various foreign substances, amino acids and vitamins, was investigated. Satisfactory results were obtained by the proposed method. The method was applied to a commercially available Ge-containing health beverage.View full abstractDownload PDF (419K) -
Hideo KUREBAYASHI, Masamichi FUKUOKA, Akira TANAKA1989 Volume 37 Issue 4 Pages 1097-1099
Published: April 25, 1989
Released on J-STAGE: March 31, 2008
JOURNAL FREE ACCESSIn order to investigate the isotope effect on the microsomal oxidative deamination of cyclohexylamine, α-deuterium(D)-labelled cyclohexylamine was synthesized. The deuterium labelling was found exclusively at the α-position with a purity of greater than 99 atom percent. Metabolic studies in vitro indicate that a significant deuterium isotope effect operates in the oxidative deamination of α-D-labelled cyclohexylamine. On incubation with rabbit liver microsomes in the presence of reduced nicotinamide adenine dinucleotide phosphate (NADPH) and oxygen, the ratio of apparent deamination rate constants (kH/kD) was 2.0±0.2 (mean±S.D.).View full abstractDownload PDF (443K) -
Toshiyuki FUJIOKA, Fumiko INOUE, Masayuki KURIYAMA1989 Volume 37 Issue 4 Pages 1100-1102
Published: April 25, 1989
Released on J-STAGE: March 31, 2008
JOURNAL FREE ACCESSTwo new affinity resins for the purification of opioid-binding materials were prepared. On was AH-Sepharose coupled with [D-Ala2, D-Leu5]enkephalin and the other was AF-Amino Toyopearl with [D-Ala2, Leu5]enkephalin. Solubilized-opioid receptors from rat brain were treated with these affinity resins and the materials with opioid-binding activities were purified. On sodium dodecyl sulfate (SDS)-polycrylamide gel electrophoresis, the purified materials showed one major band with a molecular weight of 62000-64000. The results suggested that the prepared resins are useful tools for the purification of opioid receptors.View full abstractDownload PDF (552K) -
Satoshi KOIKEDA, Masahiro TAMARU, Masuo GOTO, Nozomu MORI, Shinji TAKA ...1989 Volume 37 Issue 4 Pages 1103-1105
Published: April 25, 1989
Released on J-STAGE: March 31, 2008
JOURNAL FREE ACCESSThe effect of a change in the concentration of c-myc protein on the expression of genes for two phosphoglycerate kinase isozymes was investigated. The steady state levels of messenger ribonucleic acids (mRNAs) for sperm-type and non-sperm-type proteins were determined by blot hybridization using the RNA of the mouse cell line 38-2 containing the inducible rat c-myc gene cultured under various conditions. Without inducing the c-myc gene, mRNA for non-sperm-type protein was detected at a level that remained essentially constant during both activation and inactivation of the c-myc gene. mRNA for sperm-type protein was not detected in 38-2 cells cultured under any conditions used. Change in the amount of c-myc protein alone does not appear to bring about a switch of the expression of the two phosphoglycerate kinase genes during spermatogenesis in mouse testis.View full abstractDownload PDF (573K) -
Gaetano GIAMMONA, Libero Italo GIANNOLA, Bianca CARLISI1989 Volume 37 Issue 4 Pages 1106-1108
Published: April 25, 1989
Released on J-STAGE: March 31, 2008
JOURNAL FREE ACCESSCoupling of isoniazid with polysuccinimide afforded a water-insoluble polymeric pro-drug; by reaction with ethanolamine it was chemically transformed in a water-soluble adduct. The in vitro release of isoniazid from the drug-polymer adduct was studied by using an artificial stomach wall lipid membrane. The transfer rate constant from simulated gastric juice to simulated plasma was defined and compared with that of an equivalent dose of pure drug.View full abstractDownload PDF (513K) -
Katsuhiko OKUMURA, Hisahiro YOSHIDA, Akira KAMIYA, Ryohei HORI1989 Volume 37 Issue 4 Pages 1109-1111
Published: April 25, 1989
Released on J-STAGE: March 31, 2008
JOURNAL FREE ACCESSTo clarify the mechanism by which basic drugs accumulate in the lung mitochondria, the binding selectivity of drugs to different submitochondrial components of the perfused rat lung was examined. The accumulation of basic drugs was the highest in the mitochondrial outer membrane fraction. The drug accumulation in this fraction increased with lipid solubility and was dose-dependent. It appears then that selective binding sites for basic drugs are present in the mitochondrial outer membrane.View full abstractDownload PDF (481K) -
Toshio NISHIYAMA, Takao HAMA1989 Volume 37 Issue 4 Pages 1112-1113
Published: April 25, 1989
Released on J-STAGE: March 31, 2008
JOURNAL FREE ACCESSDrug effects are described in terms of fuzzy sets; practical application to simple cases gave reasonable results.View full abstractDownload PDF (355K) -
Fusao KAIHO, Reiko KOIKE, Hideaki NOMURA, Hiromi HARA, Kimiko MARUOKA, ...1989 Volume 37 Issue 4 Pages 1114-1116
Published: April 25, 1989
Released on J-STAGE: March 31, 2008
JOURNAL FREE ACCESSThe enhancing effect of cetyl lactate (CL) on the percutaneous absorption of indomethacin (ID) from test solutions in propylene glycol (PG) was investigated by using the abdominal skin of rats in vivo.The percutaneous absorption rate of ID from 1 or 3% CL-PG test solution through the intact skin of rats was observed to be faster than that from the control solution (without CL). The bioavailability of ID was about 0.04% for the control solution, 2.2% for 1% CL-PG and 6.8% for 3% CL-PG test solutions. These results suggest that CL functions as an enhancer for the percutaneous absorption of ID. Furthermore, marked enhancing effects on percutaneous absorption of ID were obtained at a concentration greater than 1% CL in PG.In order to elucidate the mode of action of CL as an absorption enhancer, the percutaneous absorption of ID from the control solution and 3% CL-PG test solution through damaged skin from which the stratum corneum had been stripped was additionally investigated. It was confirmed that CL acts on the stratum corneum to produce its effect.View full abstractDownload PDF (493K) -
Sakae AMAGAYA, Kazumichi HARADA, Akira MIYAKE, Hiroko IWAMA, Yukio OGI ...1989 Volume 37 Issue 4 Pages 1117-1119
Published: April 25, 1989
Released on J-STAGE: March 31, 2008
JOURNAL FREE ACCESSThe direct addition of levamisole to murine splenic lymphocytes had no effect on the mitogenic activity of lipopolysaccharide. However, the addition of serum of mice orally treated with levamisole increased the mitogenic activity, and this increased activity using serum was similar to the result obtained in an in vivo experiment. These results suggest that the new in vitro experimental method using serum may be able to reproduce the in vivo effect of drugs.View full abstractDownload PDF (557K) -
Kiyoshi TOMIOKA, Mitsuru SHINDO, Kenji KOGA1989 Volume 37 Issue 4 Pages 1120-1122
Published: April 25, 1989
Released on J-STAGE: March 31, 2008
JOURNAL FREE ACCESSBy the mediation of a chiral amino ether (4), the asymmetric α-benzylation of cyclohexanone (1) was effected via an achiral lithioenamine (6) to give α-benzylcyclohexanone (3) in up to 52% ee. A dramatic leaving-group effect of electrophiles on enantioselectivity was also observed.View full abstractDownload PDF (387K) -
Tsutomu MASUJIMA, Yumi MUNEKANE, Juing-Yi LEE, Yukio SATO, Hisanobu YO ...1989 Volume 37 Issue 4 Pages 1123-1125
Published: April 25, 1989
Released on J-STAGE: March 31, 2008
JOURNAL FREE ACCESSComputerized laser photoacoustic microscopy was developed and applied to the immuno-selective imaging of the microdistribution of a biological component. Human lambda-type light-chain was chosen as a model target protein and its peroxidase conjugated antibody was used for selective binding to the target protein and its staining. Using a microscopic calibration curve which was obtained from the images of homogeneously dispersed standard samples, the total quantities of randomly dispersed artificial standard samples were estimated to evaluate the reliability of this method. Finally, the method was applied to a tissue section sample, a human fetal pacrease section, to get the local and total quantitative images of the lambda-type light-chain.View full abstractDownload PDF (770K) -
Hiroyuki SETOI, Hiroshi KAYAKIRI, Masashi HASHIMOTO1989 Volume 37 Issue 4 Pages 1126-1127
Published: April 25, 1989
Released on J-STAGE: March 31, 2008
JOURNAL FREE ACCESSA practical synthesis of FR900490 (1__-), a peptidyl immunomodulator of microbial origin, has been achieved through diastereoselective preparation of α, β-diaminobutyric acid 2__-.View full abstractDownload PDF (313K) -
Motomichi KONO, Yutaka SAITOH, Masaji KASAI, Akira SATO, Kunikatsu SHI ...1989 Volume 37 Issue 4 Pages 1128-1130
Published: April 25, 1989
Released on J-STAGE: March 31, 2008
JOURNAL FREE ACCESSIntroducing the mercaptoethyl group at the 7-N position of mitomycin C 1 has led to the isolation of 7-N, 7'-N'-dithiodiethylenedimitomycin C 2. The compound 2 showed excellent antitumor activity against sarcoma 180 (sc-ip) and leukemia P388 (ip-ip) in mice. As an extension of this study, we synthesized mitomycin dimers with symmetrical disulfide and mitomycin derivatives with unsymmetrical disulfide at the 7-N side chain. Among these compounds, the water soluble conjugate 3 with ethyl γ-L-glyuamyl-L-cysteinylglycinate was far more effective against sarcoma 180 and leukemia P388 than 1. During the subsequent stage of inquiry for the potent congeners of 3, the compound 4 (water solubility : >500 mg/ml), designated as KW2149, with the γ-L-glutamylcystamino group at the 7th position was finally selected for further evaluation.View full abstractDownload PDF (537K) -
Isao KITAGAWA, Nam In BAEK, Kazuyoshi OHASHI, Masahiro SAKAGAMI, Masay ...1989 Volume 37 Issue 4 Pages 1131-1133
Published: April 25, 1989
Released on J-STAGE: March 31, 2008
JOURNAL FREE ACCESSFour new ionophoric resin-glycosides have been isolated from the tuber of Merremia mammosa (Convolvulaceae), an Indonesian folk medicine ("Bidara upas"). The structures of two major resin-glycosides, named mammosides B (1__∼) and H1 (2__∼), have been determined on the basis of chemical and physicochemical evidence including a synthesis of the glycosidic acid designated as mammoside I (3__∼).View full abstractDownload PDF (465K) -
Tsunehiro KITAGAWA, Hideaki TANIMORI, Yohko KOHNO, Akira AKAHORI, Shin ...1989 Volume 37 Issue 4 Pages 1134-1136
Published: April 25, 1989
Released on J-STAGE: March 31, 2008
JOURNAL FREE ACCESSAntiserum specific to Pinellia tuber was elicited in a rabbit. The extract of the tuber was converted to solid-phase antigen using microtiter plates. A new method for enzyme immunoassay of the tuber extract was developed with an antiserum to the tuber and a solid-phase antigen as immunological reagents, and β-D-galactosidase-labeled anti-rabbit IgG as the tracer. The method was successfully applied to the quantitative analysis of the extracts of Pinellia tuber contained in various chinese tradiational medicines. The specificity of the assay to the extracts was also demonstrated.View full abstractDownload PDF (436K) -
Atsuko ITOH, Takao TANAHASHI, Naotaka NAGAKURA1989 Volume 37 Issue 4 Pages 1137-1139
Published: April 25, 1989
Released on J-STAGE: March 31, 2008
JOURNAL FREE ACCESSTwo new monoterpene glucosides with unusually 7, 8-disubstituted tetrahydroisoquinoline nucleus, neoipecoside and 7-methylneoipecoside, have been isolated from Cephaelis ipecacuanha A. Richard. Their structures were elucidated as 1 and 2 by spectroscopic and chemical means.View full abstractDownload PDF (424K) -
Kazuo MASUDA, Kenji SHIOJIMA, Hiroyuki AGETA1989 Volume 37 Issue 4 Pages 1140-1142
Published: April 25, 1989
Released on J-STAGE: March 31, 2008
JOURNAL FREE ACCESSFrom the fresh whole plants of Lemmaphyllum microphyllum var. obovatum (Polypodiaceae), we isolated two new oily tricyclic triterpenoid hydrocarbons, 13βH-malabraicatriene (1) and 13αH-malabaricatriene (2), having the malabaricane skeleton. Their structures were established by physicochemical methods. This is first example of the isolation of tricyclic triterpenoid hydrocarbons from a natural source.View full abstractDownload PDF (462K)