巻号一覧
20 巻, 1 号
選択された号の論文の5件中1～5を表示しています
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II. BCG菌体から抵抗性増強物質の分離について土屋 皖司, 山崎 俊幸1965 年 20 巻 1 号 p. 1-4
発行日: 1965/01/25
公開日: 2009/02/19
ジャーナル フリーSeparation of active substance from BCG cells for heterologous immunity was tried.
Free and bound-lipids were extracted by the method of Ledere and Asselineau. Thereafter, the defatted cells were treated first in the buffer of pH 4, then pH 7 and finally pH 9 for fractionation, followed by the treatment of Pronase-P. The enzyme treated bacterial residue was oxidized with NaIO4 and then reduced with NaBH4.
The presence of heterologous immunogenicity in each fraction was tested, comparing with intact cells. Mice injected with Drakeol suspended fraction intraperitoneously were challenged with intravenous staphylococcal infection.
Heterologous immunogenic activity was recognized in the residual fraction of NaIO4-NaBH4 treatment.
The activity was also noted in phenol-extracted residue, urea extracted residue and cell wall. Through these finding, the heterologous immunogenic substance of BCG cells is different from the known lipopolysoccharide of Gram-negative bacteria or zymosan.抄録全体を表示PDF形式でダウンロード (399K) -
レイ菌およびレイ菌感染症斉藤 慶一1965 年 20 巻 1 号 p. 5-13
発行日: 1965/01/25
公開日: 2009/02/19
ジャーナル フリーThe isolation of six strains of Serratia marcescens from infections subsequent to massive administration of antibiotics is reported in this paper: from three cases of respiratory tract infection, from two cases of post-operative infection, and from one case of intestinal tract infection.
And, some evidence in the etiological role in the infection due to Serratia marcescens is presented from the point of resistance to antimicrobial agents.抄録全体を表示PDF形式でダウンロード (1169K) -
1. サルへの経口感染とDe-testについて寺山 武1965 年 20 巻 1 号 p. 14-20
発行日: 1965/01/25
公開日: 2009/02/19
ジャーナル フリーFor the purpose of determining the pathogenicity of Vibrio parahaemolyticus, the author intended to made 2 experiments on the bacterial cultures derived from the same strains. viz., 1) The oral administration test of V. parahaemolyticus in monkeys. 2) The De-test, a unique inoculation technique using a live rabbit small intestine. In this test the animals were sacrificed at the certain intervals of time, then observed both visualy and histopathologically.
The results obtained are as follows:
1) None of 14 monkeys showed any clinical symptoms suggesting enteritis, which occured in the case of human enteritis caused by this organisms.
2) In the autopsy of the De-test on the rabbit, it is observed that the exudate began to accumurate in the ligated loop of the intestinal tract, at about 6 hours post-inoculation and remarkable edema was appeared. The degeneration of the mucous membrane cells were observed histopathologically at about 9-12 hours, followed by the necrotic changes.
3) The results of De-test observed at 24 hours post inoculation of V. parahaemolyticus were resembling to that of induced by El Tor vibrio.
4) It is presumable from the results of De-test that the pathogenic substance of this organism is a relatively thermolabile component of the bacterial cell.
5) It was clarified that the De-test is one of the useful procedures for judging the pathogenicity of V. parahaemolyticus.抄録全体を表示PDF形式でダウンロード (2803K) -
1. 琉球列島のハブ属の毒の比較貞弘 省二, 山内 清澄, 近藤 了, 近藤 久, 村田 良介1965 年 20 巻 1 号 p. 21-26
発行日: 1965/01/25
公開日: 2009/02/19
ジャーナル フリーComparisons were made on the lethal and hemorrhagic activities and immunological specificities of venoms from the snakes belonging to genus Trimeresurus (Crotalidae).
The venoms from T. flavoviridis found in the Amami Islands, the island of Okinawa and Kume, and from T. elegans in the Yaeyama Islands were not different significantly with respect to the specific lethal and hemorrhagic activities. The specific activities of the venoms from T. okinavensis in the Amami Islands and from T. tokarensis in the Tokara Islands were lower than those of the four different snake venoms of the two species mentioned above.
The neutralization tests, agar-gel diffusion and immuno-electrophoresis, using an antivenine against Habu venom from the Amami Islands, indicated little difference in immunological specificities among the venoms from T. flavoviridis collected in different islands.
The hemorrhagic activities of the venoms from T. elegans, T. okinavensis and T. tokarensis were neutralized by the Habu antivenine, while the lethal activities of these were not neutralized. Immunological specificities of these venoms, as revealed by the immuno-electrophoresis and agar-gel diffusion tests, differed from that of T. flavoviridis.
In view of the above findings, the specific antivenine against each venom should be administered for the treatment of poisoning by venom of any species of genus Trimeresurus.抄録全体を表示PDF形式でダウンロード (2064K) -
V. 無処置のまま或いは種々な前処置を施こし生菌少量投与ならびに破壊菌の胃内経口投与をおこなつた幼若サルの臨床所見ならびに剖検所見林 長男, 岩原 繁雄1965 年 20 巻 1 号 p. 27-30
発行日: 1965/01/25
公開日: 2009/02/19
ジャーナル フリーOn the experiments of Shigella infections, normal and pretreated animals were infected with multiple drug-resistant strains of Snigella flexneri 2a (Komatsu strain) or 3a (Miyata strain) by its introduction directly into the stomach.
The pretreatments which the animal received were as follows.
(1) Oral administration with CM and CaCO3 into the stomach.
(2) Intravenous injection of sonicate (50mg, wet weighit).
(3) Injection of sonicate (400mg, wet) into the duodenum.
On the other experiments, the only sonicate of Shigella flexneri (26.7g, wet) or French press destroyed organisms (165mg, wet) were introduced directly into their stomach.
The total animals used in these experiments were ten young monkeys weighing about 1kg.
Number of live organisms introduced were 2.5×105∼2.4×108.
The results were as follows.
(1) The animal (M17) was injected directly with the sonicate (400mg, wet) into the duodenum. 5 hours later, it was introduced with living cultures of 6×106 into the stomach.
This animal showed fatal changes. It was considerd that the symptom was more serious than dysentery
(2) Symptoms of dysentery were not observed with the animal (M16) which is injected intraveously with sonicate (50mg, wet) and body temperature fell down from 38.9°C to 35.6°C within a few minutes after the injection.
(3) Clinical changes were not observed with other animals.抄録全体を表示PDF形式でダウンロード (515K)
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