巻号一覧
19 巻, 10 号
選択された号の論文の3件中1～3を表示しています
- |<
- <
- 1
- >
- >|
-
第2報 BCGのリボゾームの分離とその性状江田 亨1964 年 19 巻 10 号 p. 411-417
発行日: 1964/10/25
公開日: 2011/06/17
ジャーナル フリーIn the previous communication, it was demonstrated that the ribosome fraction was obtained from the cell-free extract of Mycobacterium 607 by preparative ultracentrifugation.
This paper deals with isolation procedure and characterization of the ribosome of BCG.
BCG was cultured with a Tween synthetic medium at 37°C for 14 days. The cells were harvested by centrifugation, and washed with and resuspended in the 0.01M tris buffer containing 0.01M of MgCl2(tris-Mg buffer). The cell-free extract of the washed cells was prepared by passing through the French pressure cell at 400Kg/cm2 followed by centrifugation at 5, 000g for 20minutes.
Particulate fractions were isolated from the cell-free extract by a successive ultracentrifugation at 20, 000g for 60 minutes (20p60 fraction), 60, 000g for 90 minutes (60p90 fraction) and 105, 000g for 120 minutes (105p120 (I) fraction). Final supernatant fraction (105s120 (I) fraction) was used as a soluble enzyme. In some experiments, the ribosome fraction was directly obtained by supercentrifugation at 105, 000g for 120 minutes (105p120 (II) fraction) after removal of 20p60 fraction from the cell-free extract by centrifugation. Each particulate fraction was suspended in the tris-Mg buffer, and analyzed physicochemically and biologically. Results obtained were as follows:
1) All of the 60p90, 105p120 (I) and 105p120 (II) fractions were found to contain a higher amount of nucleic acid than 105s120 fraction U-V spectrophotometorically.
2) The RNA and protein ratios in the 60p90, 105p120 (I) and 105p120 (II) fractions were 58, 8: 41, 2, 51, 5: 48, 5 and 61, 3: 38, 7, respectively.
3) As observed by analytical centrifugation patterns, the 60p90, 105p120 (I) and 105p120 (II) fractions consisted of 71, 4 S and 48, 3 S particles.
4) An electronmicrograph of the 105p120 (II) fraction demonstrated globular granules with an average diameter of 30-40mμ.
5) The 105p120 (II) fraction was capable to incorporate14C-amino acids into its protein fraction in the presence of ATP and its generator, 105s120 fraction, GTP and GSH.
These results may indicate that the 60p90, 105p120 (I) and 105p120 (II) fractions are the ribosome fractions of BCG.抄録全体を表示PDF形式でダウンロード (4688K) -
第1報 腸炎ビブリオ抽出毒性分画の精製と化学的性状ならびに生物活性について栗山 一夫1964 年 19 巻 10 号 p. 418-424
発行日: 1964/10/25
公開日: 2011/06/17
ジャーナル フリーThere were many studies for Vibrio parahaemolyticus since it was discovered by Fujino et al in 1950.
The author has also studied to clarify the properties of this organism, and found that this organism had a toxic component in its thick envelope. The slime layer of the envelope was isolated and purified. The purified fraction was found to be toxic to mice. The LD50 of the toxic fraction by intraperitoneal injection was about 70 mcg per mouse weighing of 15 gms. This toxic fraction which have a peak at 260 m & mu; in optical density was not inactivated with neither trypsin nor RNase, and its toxicity to mice lost by the enzymatic action of DNase. From the physico-chemical examination, the isolated toxic fraction might be almost purified DNA. These results suggest that the envelope substance of Vibrio parahaemolyticus might have a significant role and its toxic component might be DNA of the organism.抄録全体を表示PDF形式でダウンロード (4439K) -
勅使河原 正男1964 年 19 巻 10 号 p. 425-433
発行日: 1964/10/25
公開日: 2011/06/17
ジャーナル フリーIn an attempt to isolate Rickettsia orientalis from rodents by means of tissue culture method, a study with a number of growing cell culture in liquid medium was performed to find out the optimal conditions for the support of the growth of R. orientalis from the liver and spleen of the mice previously infected with this infectious agent. The following results are obtained.
1) When the suspension of the infected liver and spleen at a concentration of 10% is seeded onto the cells and incubated for 60 minutes at 37°C., it was observed that one-tenth of the particles of the seeded agents was absorved.
2) Among the cell cultures used, a noticeable difference is not observed in the growth of R. orientalis, although a slightly lower LD50 titer is estimated in tissue cultures with the culture of DK cells.
3) R. orientalis can be easily cultivated and isolated from the specimens from the trapped rodents under the conditions described above.
4) For long period cultivation and for the study of the fine morphological structure of R. orientalis, tissue cultures with Descement cells seems to be the most applicable.抄録全体を表示PDF形式でダウンロード (3410K)
- |<
- <
- 1
- >
- >|