巻号一覧
21 巻, 6-7 号
選択された号の論文の6件中1～6を表示しています
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小野瀬 英雄, 佐々木 市郎, 金 兌貞1966 年 21 巻 6-7 号 p. 307-311
発行日: 1966/07/25
公開日: 2011/06/17
ジャーナル フリーOn the materials gathered from sewages, salivas, dental plaques, and feces of sucklings which may contain lactobucillus, isolation of phage was done. A phage “N-1” was isolated from a material of the sewage.
N-1 phage should have biological characters as followed when examined with L. casei, strain L-10 in L-broth: adsorption rate 73 % at 37°C for 10 minutes, latent period 150 minutes, average but size 46 per cell.
Plaque formation of N-1 phage disturbed by addition of citrate-ion to L-A media as formed less than 1/5 of plaque number of the control. Addition of Ca-ion is essential to have better efficiency of plating.
Survival of N-1 phage in L-broth should decreased to less than 0.1 per cent at 60°C for 5 minutes. Of 40 lactobacillus strains, sensitivity were examined against N-1 phage and J-1 phage which was donated from the Yakuruto Institute. A few strains were detected as sensitive ones against either one of N-1 or J-1 phages respectively.抄録全体を表示PDF形式でダウンロード (1952K) -
とくにマウス対S. enteritidisの組みあわせにおいて三橋 進1966 年 21 巻 6-7 号 p. 312-328
発行日: 1966/07/25
公開日: 2011/06/17
ジャーナル フリーWhen the mice were immunized with live vaccine of Salmonella enteritidis or survived the infection with virulent strain of S. enteritidis by the aid of antibiotics, mice acquired high antilethal resistance against further infection with 1, 000 MLD of a virulent strain 116-54 of the same organism. In contrast, killed vaccines increased the survival time of mice after challenge but were largely ineffective in preventing ultimate death from the infection.
The mononuclear phagocytes, obtained from the abdominal cavity, subcutaneous tissue, or liver of mice immunized with live vaccine, inhibited the intracellular multiplication of bacteria and resisted cell degeneration caused by engulfment of bacteria, without the presence of immune sera in cell culture medium, but the mononuclear phagocytes obtained from the mice immunized with killed vaccines did not. This resistance of immune mononuclear phagocytes is referred to as cell immunity.
The cell-bound antibody is detectable in the mononuclear phagocytes of immunized mice and inhibited the growth of a virulent strain 116-54 with the aid of complement and lysozyme, either on the nutrient agar plate or in the non-immunized mononuclear phagocytes. This antibody is extracted from the immune mononuclear phagocytes or the spleen of immunized mice and is found to be macroglobulin.
The cell immunity is transferred from immune mononuclear phagocytes to nonimmune cells through the transfer agent (TA) of cell immunity. The TA is of RNA nature and detectable in the cell culture fluid or microsomal (or ribosomal) fraction of immune mononuclear phagocytes. When the normal mononuclear phagocytes were treated with TA, the cells acquired cell resistance against infection and inhibited intracellular multiplication of bacteria. Also, the cell-bound antibody was formed in the cells treated with TA. These facts strongly suggest that TA is a messenger of antibody formation or a carrier of antigenic information.
According to the results described above, the author has presented the new cell line for the antibody formation, i. e., the role of mononuclear phagocytes in antibody formation and of the formrtion of TA after taking in of antigen, beside the plasma cellular and lymphoreticular cell lines for the antibody formation.抄録全体を表示PDF形式でダウンロード (5151K) -
添田 百枝1966 年 21 巻 6-7 号 p. 329-331
発行日: 1966/07/25
公開日: 2011/06/17
ジャーナル フリーFrom 1936 onwards, Hosoya et al had prepared purified staphylococcal toxoid from cultures of sensitive staphylococcal strains and used it as a prophylactic and therapeutic agent against staphylococcal infections, with a striking beneficial effect on acute and chronic cases. Although this method threw much light upon the therapeutic aspects of such infections, the precise mechanism by which it can contribute much to inhibition of staphylococcal infections has been almost entirely unknown since that time. Difficulties in this elucidation of the mechanism arose from the fact that a parallel correlation between clinical improvement and elevation of antitoxin (anti-hemolysin) titers in human sera could not have been achieved in so many cases.
Recently Soeda and Kitahara found a close relationship between inhibition of staphylococcal infections and the titers of specific precipitin in sera of both human and animal hosts. The patterns of variation in precipitin titers of host sera tend to well correlate with those of alteration in clinical response to toxoid therapy. It seems reasonable to consider that titration of antihemolysin induced by toxoid injection may contribute little, if any, to evaluation of therapeutic effect of toxoid and that titration of specific precipitin in host sera may be a more useful measure for the same purpose. Here I will report on the experimental results by which foregoing our views may be supported.抄録全体を表示PDF形式でダウンロード (433K) -
添田 百枝1966 年 21 巻 6-7 号 p. 332-335
発行日: 1966/07/25
公開日: 2011/06/17
ジャーナル フリーFrom about 1936 to 1944, Hosoya et al had coducted extensive studies on staphylococcal toxoid, including production of exotoxin and its chemical purification and clinical application of toxoid to human staphylococcal infections. In pre-antibiotic years, this clinical use of toxoid was a sole potential medical measure for treatment of supprative staphylococcal infections, and it threw much light upon therapeutic aspects of so many staphylococcal diseases. The discoveries of various antibiotics including Penicillin since 1948, however, have remarkably reduced the therapeutic value of staphylococcal toxoid because of its delayed type of effectiveness, but subsequent appearance of multi-resistant strains of staphylococci has become an insuperable obstacle in antibiotic therapy for staphylococcal infections. Recently, highly purified staphylococcal toxoid was prepared from cultues of multi-resistant staphylococcal strains in our laboratory and striking beneficial effect was recognized in practical therapy of various forms of resistant staphylococcal infections against antibiotics. I will report here on cases of such infections in which toxoid induced a remarkable therapeutic effect leading to complete recovery and a long lasting immunity against staphylococcal infections.抄録全体を表示PDF形式でダウンロード (618K) -
Coccaceae科のStreptococceae族について林 江沢, 小平 富子, 菊地 和子, 馬場 久美子1966 年 21 巻 6-7 号 p. 336-340
発行日: 1966/07/25
公開日: 2011/06/17
ジャーナル フリーA new classification was proposed from the view point of relationship among microoganisms, which was based on the Adanson's thinking, Sneath's similarity value and the author, Hayashi's concept of center species.
According to this method, some reorientation for the classification of the tribe Streptococceae and the classical family Lactobacillaceae was made and the following results were obtained.
1) The classical genus Diplococcus should be integrated to the genus Streptococcus, because of the high similarity value to many species of the genus Streptococcus and the center species, Streptococcus bov is.
2) Peptostreptococcus magnus should be omitted from the genus Peptostreptococcus and belongs to the genus Peptococcus, because of the lower similarity value to the center species of Peptostreptococcus and a higher similarity value to the center species of Peptococcus.
3) The other species of genus Peptostreptococcus are proper to constitute the genus Peptostreptococcus. The center species of the genus Peptostreptococcus is Peptostreptococcus productus.
4) Taking into consideration the three center species, based on the reciprocal mean similarity value and taxonomic rank proposed by the author, it is proper that Leuconostoc, Streptococcus and Peptostreptococcus constitut the tribe Streptococceae.
5) The tribe Lactobacilleae indicats a slightly higher similarity value to the classical family Propionibacteriaceae than to the tribe Streptococceae.
6) It is proper that the tribes Micrococceae, Sarcineae, Streptococcae and Neisserieae constitute the family Coccaceae.抄録全体を表示PDF形式でダウンロード (910K) -
木村 正健1966 年 21 巻 6-7 号 p. 341-350
発行日: 1966/07/25
公開日: 2011/06/17
ジャーナル フリーInvestigations on the improvements of the techniques of haemagglutination test for the differentiation of vibrios were made using strains of V. cholerae, V. eltor, NAG vibrio, V. parahaernolyticus and V. alginolyticus.
As the suspending medium of the red blood cells, PBS (pH 7.6, for tissue culture) was most excellent. One drop of 3% red blood cell suspension in PBS at pH 7.6 was placed on the glass slide, and sufficient amount of test organism (about 0.5mg) was rubbed into the drop with the loop and mixed well; then, visible clumping took place almost immediately.
As for the species of the red blood cells, guinea pigs were most preferable; chick, rabbit and horse were next in order; sheep and ox were not found suitable.
Test organisms grown in the media in which acidification takes place after the bacterial growth should not be used for this test. V. eltor cells grown in such condition failed to cause positive clumping.
Barua and Mukerjee (1965) stated that red blood cells of guinea pigs are inadequate for this test, because of the occasional positive clumping in strains ofV. cholerae.However, according to the results of this study, visible clumpings were also observed with red blood cells of the other species such as horse, rabbit and chick. Continued observation of the same strains of V. cholerae revealed that in some straips of V. cholerae haemaggliutination test turned to positive during the course of subculture, indifferent to S-R variation.抄録全体を表示PDF形式でダウンロード (1564K)
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