The methanol extract from
Alpinia oxyphylla showed a suppressive effect on
umu gene expression of SOS response in
Salmonella typhimurium TA1535/pSK1002 against the mutagen 2-(2-furyl)-3-(5-nitro-2-furyl)acrylamide (furylfuramide). The methanol extract from
A. oxyphylla was successively re-extracted with CH
2Cl
2, EtOAc, BuOH, and water. A dichloromethane fraction showed a suppressive effect. The suppressive compound in the dichloromethane fraction was isolated by SiO
2 column chromatography and identified as yakuchinone A(
1) by GC, GC/MS,
1H and
13C NMR spectroscopy. Compound
1 suppressed the SOS-inducing activity of furylfuramide in the
umu test. Compound
1 suppressed 51.7% of the SOS-inducing activity at a concentration of 0.32μM/mL. The ID
50 value (50% inhibition dose) of compound
1 was 0.30μM/mL. This compound was assayed with an other mutagen, 3-amino-14-dimethyl-5
H-pyrido[4,3-
b]indole (Trp-P-1), which requires a liver metabolizing enzyme, activated Trp-P-1.
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