On the basis of reports that a minor groove binder pyrrolepolyamide can interfere with gene expression by the sequence-specific recognition of DNA, we expected that nucleoside bearing a pyrrolepolyamide would be able to regulate gene expression. Therefore, we designed and synthesized the pyrrolepolyamide-adenosine (
Hybrid 1) and -2′-deoxyguanosine hybrids (
Hybrid 2 and
Hybrid 3) as lead compounds for gene expression control compounds. The pyrrolepolyamide frame of
Hybrid 2 and
Hybrid 3 combines at the 2-exocyclic amino group of the 2′-deoxyguanosine by a linker and the 2-exocyclic amino group of guanine exists in the minor groove side of the duplex.
Hybrid 2 is the 2′-deoxyguanosine-pyrrolepolyamide hybrid using the 3-aminopropionyl linker, while
Hybrid 3 uses the 3-aminopropyl linker. An evaluation of the DNA binding sequence selectivity was performed by analysis of
Tm values and CD spectra, using distamycin A as a contrast.
Hybrid 3 has provided more excellent sequence-distinguishable ability than other hybrids and Distamycin A. Moreover, on the basis of these results, we synthesized oligonucleotides conjugated to
Hybrid 4, which is stable under conditions of DNA oligonucleotide solid phase synthesis, arranged from
Hybrid 3. From
Tm values and CD spectral analysis, it was found that oligonucleotides conjugating
Hybrid 4 possess high recognition ability and very high binding ability for the DNA that includes the pyrrolepolyamide binding sequence.
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