YAKUGAKU ZASSHI
Online ISSN : 1347-5231
Print ISSN : 0031-6903
ISSN-L : 0031-6903
98 巻 , 8 号
選択された号の論文の23件中1~23を表示しています
  • 中川 寛, 宮田 竜彦, 毛利 邦子, 杉本 功, 真鍋 秀幸
    1978 年 98 巻 8 号 p. 981-985
    発行日: 1978/08/25
    公開日: 2008/05/30
    ジャーナル フリー
    Presence of anhydrous form and three hydrates of berberine chloride was confirmed by thermogravimetric analysis, differential thermal analysis, and X-ray diffractometry. The anhydrous form and monohydrate were very hygroscopic, and easily transformed to dihydrate at 12% relative humidity. Dihydrate was relatively hygroscopic and transformed to tetrahydrate at a high relative humidity. Tetrahydrate was transformed to dihydrate at 40-70°, to monohydrate at 80°, and to anhydrous form at above 90°. It was found that under ordinary storage condition, anhydrous form and monohydrate could not exist because of their remarkable hygroscopicity, and dihydrate and tetrahydrate were the stable phase. Commercial berberine chloride was a mixture of dihydrate and tetrahydrate.
  • 松浦 巌, 川真田 正信
    1978 年 98 巻 8 号 p. 986-996
    発行日: 1978/08/25
    公開日: 2008/05/30
    ジャーナル フリー
    Theoretical equations for the prediction of moisture sorption of pharmaceutical preparations under various conditions were derived and evaluated. They are useful for the design of moisture-proof packaging and the quality assurance of pharmaceutical preparations, as moisture sorption is one of the factors which affect physical properties, appearance, and chemical stability of pharmaceutical preparations. Moisture sorption under the shelf condition can be predicted accurately when the temperature change, relative humidity change, moisture sorption isotherm, activation energy of permeability, and heat of vaporization are taken into consideration. A method for the estimation of moisture sorption rate constant of pharmaceutical preparations is also described. Experiments were carried out for moisture sorption of packaged salt, plain tablets, or sugar-coated tablets under various conditions, and it was proved that calculated values were in good agreement with the observed values.
  • 篠田 雅人, 清水 素行, 櫛 泰典, 中陳 静男
    1978 年 98 巻 8 号 p. 997-1004
    発行日: 1978/08/25
    公開日: 2008/05/30
    ジャーナル フリー
    A factor inhibiting the leukocytosis-promoting activity of parotin was isolated from rabbit serum and the nature of this factor was examined, with following results. 1) Inhibition of the activity by rabbit serum was observed by treatment at 37°for 15 min, and a definite inhibition was seen when treated for over 60 min. 2) This parotin-inhibitory factor is stable to heating at 56°for 30 min but loses its activity when heated at 100°for 5 min. 3) This inhibitory factor was purified by ammonium sulfate fractionation, gel filtration over Sephadex G-200 and Sepharose 6B, chromatography over DEAE-cellulose, and isoelectric focusing in carrier ampholyte, and a substance homogeneous in disc electrophoresis and isoelectric focusing in carrier ampholyte was separated. The molecular weight of this substance was 210000 by gel filtration over Sephadex G-200, 190000 by gelfiltration over Sepharose 6B, and 160000 by the Hedrick-Smith method. It had an isoelectric point at pH 5.8. 4) This parotin-inhibitory factor seemed to have an inhibitory action different from antigen-antibody reaction or proteinolytic reaction.
  • 徳野 健次, 三由 文久, 荒田 義雄, 板谷 芳京, 荒川 良夫, 大橋 力
    1978 年 98 巻 8 号 p. 1005-1011
    発行日: 1978/08/25
    公開日: 2008/05/30
    ジャーナル フリー
    Solutions of II (ylid of I) were prepared by treatment of 1-thioniabicyclo [4. 4. 0] decane bromide (I) with sodium hydride in nitrogen at refluxing temperature in abs. tetrahydrofuran. The reaction of II with carbonyl compounds and active methylene compounds was examined and in all cases examined, II reacted with the carbonyl and active methylene compounds at its 2-position to give the corresponding epoxides and monosubstituted active methylene compounds, respectively. The reaction of II with 1/2 molar equivalent of malononitrile gave a disubstituted malononitrile in a good yield.
  • 森山 圭雄, 井上 顕信, 磯矢 誠, 田中 正生, 花野 学
    1978 年 98 巻 8 号 p. 1012-1018
    発行日: 1978/08/25
    公開日: 2008/05/30
    ジャーナル フリー
    Physicochemical properties of coprecipitate of chloramphenicol (CP)-polyvinylpyrrolidone (PVP) and CP-hydroxypropylcellulose (HPC) were investigated. The dissolution rate and apparent solubility of CP from the coprecipitate increased in the CP-PVP system, but the increase of apparent solubility was not significant in CP-HPC system. IR spectral changes due to the possible formation of hydrogen bonds were observed in the coprecipitate of CP-PVP system, but not in that of CP-HPC system. The CP in the coprecipitate was found to be an amorphous form by the differential scanning calorimetry and X-ray diffraction measurement. In CP-PVP system, difference in properties between the coprecipitate and physical mixture was always found when the mixing ratio (w/w) of CP : PVP was less than 7 : 3. The absorption of CP after oral administration of the coprecipitate powder and pellet in the dog was 1.2-1.9 times greater in area under plasma level-time curve (0-9 hr) than that of the mixture.
  • 堀 幹夫, 片岡 貞, 清水 洋, 今井 豊, 藤村 一
    1978 年 98 巻 8 号 p. 1019-1027
    発行日: 1978/08/25
    公開日: 2008/05/30
    ジャーナル フリー
    A general method has been established for useful synthesis of benzothiazoline derivatives possessing various functional groups. Reduction of benzothiazolium salts with sodium borohydride in pyridine and reaction of these salts with Grignard agents were examined and many 2, 3-disubstituted and 2, 2, 3-trisubstituted benzothiazoline derivatives were synthesized. Among the benzothiazoline derivatives so obtained, some analgesic and antiinflammatory activities were found in 3-methyl-2-phenyl- and 2, 3-dimethyl-2-phenyl-5-benzothiazolineacetic acids.
  • 清水 忠順, 原口 惣一, 三渕 一二, 中野 昌康
    1978 年 98 巻 8 号 p. 1028-1034
    発行日: 1978/08/25
    公開日: 2008/05/30
    ジャーナル フリー
    Yeast cell wall (YCW) or its polysaccharide (glucan), when inoculated subcutaneously as a mixture with Ehrlich ascites tumor cells into mice, remarkably suppressed the tumor growth at the injected site. However, both water-solubilized (enzyme digested) glucan and mannan obtained from YCW were difficult to show effective suppression on the tumor growth. Tumor suppressive effect of YCW in mice was markedly diminished by previous treatment of mice with either anti-thymocyte serum (ATS) or X-ray irradiation. Weeks after the inoculation of YCW-tumor cell mixture, these mice showed high resistance to growth of the same tumor cells, if reinoculated. Thus, these mice were termed"tumorsuppressed mice."Higher doses (800-900 R) of irradiation or ATS administration to the"tumor-suppressed mice"could completely abolish their resistance to the tumor cells, while low doses (400-500 R) of irradiation could still preserve their ability to inhibit the tumor growth to some extent. Neutralizing test in vivo revealed that the sensitized lymphocytes obtained from the mice which had been inoculated with YCW-tumor cell mixture several weeks in advance had some ability to suppress the growth of inoculated tumor cells. These results suggest that both phenomena, the tumor suppressive effect of YCW and the growth inhibitory effect in"tumor-suppressed mice"are related to the immunological capability of the host.
  • 清水 忠順, 原口 惣一, 三渕 一二
    1978 年 98 巻 8 号 p. 1035-1040
    発行日: 1978/08/25
    公開日: 2008/05/30
    ジャーナル フリー
    To investigate the role of lymphocytes on the antitumor activity of yeast cell wall (YCW) in mice, functional analysis of lymphocytes was made on the basis of the mitogenic response (3H-thymidine uptake) of mouse spleen cells to bacterial lipopolysaccharide (LPS ; a B cell mitogen) and Concanavalin A (Con A ; a T-cell mitogen). When normal mice and"tumor suppressed mice"(the mice in which tumor growth had been suppressed by the mixed injection of YCW and Ehrlich ascites tumor cells : EATC) were administered anti-thymocyte serum (ATS) or irradiated with X-rays, the tumor suppressive effect of YCW and their acquired resistance against tumor transplantation decreased. By the administration of ATS into these mice, the response of their spleen cells to Con A was reduced, but the response to LPS was not. X-Ray irradiation resulted in the reduction of mitogenic responses to both Con A and LPS. These results suggest that the tumor suppressive effect of YCW is mediated by T-cell function in mice and the acquired tumor resistance is also dependent on their T-cell function. The mitogenic activity of YCW was confirmed on cultured spleen cells in vitro. The spleen cells from the mice 2 days after the administration of YCW showed a marked enhancement of the mitogenic response to LPS. This fact indicates that YCW is capable of increasing B-cell population and/or the mitogenic responsiveness of B-cells in mouse spleen. The change of mitogenic response of spleen cells to LPS, Con A and Mitomycin-C treated EATC (MMC-EATC) during process of tumor suppression after the injection of mixture of YCW and EATC were investigated. Two days after the injection, the enhancement of mitogenic responses to LPS and MMC-EATC were observed. On 28 days, when the growth of tumor was mostly suppressed, the mitogenic response of their spleen cells to Con A was incresed. These finding suggest that YCW stimulates the B-cell at the early stage after the injection, and then converts its stimulation into T-cell in the advanced stage. The enhancement of T-cell function in the advanced stage be YCW may relate to its ability on anti-tumor activity.
  • 有吉 敏彦, 大平 健一, 吉武 早苗
    1978 年 98 巻 8 号 p. 1041-1047
    発行日: 1978/08/25
    公開日: 2008/05/30
    ジャーナル フリー
    After simultaneous administration of cadmium chloride and lead acetate to rats, the tissue concentration and excretion of the metal, as well as cadmium-binding proteins in the liver and kidney supernatant fractions, was examined. 1) Content of cadmium in the liver, kidneys, and bone decreased to a greater extent by the simultaneous treatment with cadmium chloride and lead acetate than by the treatment with cadmium chloride alone. 2) Fecal and biliary excretion of cadmium also decreased by the simultaneous treatment with cadmium chloride and lead acetate, while the content of lead was increased in feces. 3) After treatment with cadmium chloride alone, cadmium in the liver supernatant fraction was present in both positions of low-molecular protein (cadmium-binding protein) and of considerably high-molecular (about 20000-30000) protein fractions. After simultaneous treatment with cadmium chloride and lead acetate, lead was concentrated markedly in the low-molecular protein fraction instead of cadmium, although lead was bound little to that position after treatment with lead acetate alone. In the kidney supernatant fractions, cadmium was present in the low-molecular fraction, and this localization was not affected by simultaneous treatment.
  • 真田 修一, 庄司 順三, 柴田 承二
    1978 年 98 巻 8 号 p. 1048-1054
    発行日: 1978/08/25
    公開日: 2008/05/30
    ジャーナル フリー
    A new method for quantitative analysis of ginseng saponins, ginsenoside-Ro, -Rb1, -Rb2, -Rc, -Rd, -Re, -Rf, -Rg1, and -Rg2, was developed by the combination of thin-layer chromatography (TLC) and spectrometry. The 90% methanol extract of ginseng and pure standard ginsenosides were spotted on the same two TLC plates ; one plate was developed with chloroform- methanol-water (65 : 35 : 10, lower phase) and the other with chloroform-butanol-methanol-water (20 : 40 : 15 : 20, lower phase). After drying, both plates were sprayed with 10% sulfuric acid solution followed by heating, and each ginsenoside spot was measured by TLC densitometer using dual-wave length TLC scanner. Reasonable recovery and standard deviation were found in this procedure and the contents of each ginsenoside in ginseng and its congeners, American ginseng, San-chi ginseng, were determined by applying the described method.
  • 瀬山 義幸, 山下 三郎, 石川 信雄
    1978 年 98 巻 8 号 p. 1055-1062
    発行日: 1978/08/25
    公開日: 2008/05/30
    ジャーナル フリー
    Decarboxylation of iodohistidines and histidine was comparatively examined by measurement of 14CO2 formation from 14C-iodohistidine (14C-MIH, 14C-DIH) and 14C-histidine during incubation with bacterial histidine decarboxylase or with rat liver homogenate. The decarboxylation rate of iodohistidines was higher than that of histidine, and the decarboxylation of DIH was much faster than that of MIH or histidine, without showing any time-lag. It may be suggested that iodohistidines are directly decarboxylated without deiodination by decarboxylase (s) and converted into the corresponding iodohistamines, which were identified with separately synthesized iodohistamines, by thin-layer chromatogram on cellulose, and it was found that the ratio of decarboxylated metabolites (iodohistamines) was higher than that of deiodinated products (MIH and histidine), and that deaminated metabolite (urocanic acid) was not detected in the metabolites. It was shown that decarboxylation is the major pathway of iodohistidine metabolism. Iodohistidines seemed to be decarboxylated non-specifically by enzymes (histidine decarboxylase, aromatic L-amino acid decarboxylase) in liver homogenate, since the decarboxylation rate of iodohistidines is higher in alkaline pH (indicating aromatic L-amino acid decarboxylase), but it is inhibited slightly by α-methyl-DOPA, an inhibitor of the enzyme.
  • 亀谷 哲治, 気賀沢 和雄, 柊木 峯治, 脇坂 菊雄, 芳賀 清次, 草間 攻, 杉 秀夫, 谷川 啓造
    1978 年 98 巻 8 号 p. 1063-1071
    発行日: 1978/08/25
    公開日: 2008/05/30
    ジャーナル フリー
    Cyclization of diethyl N-(5-indazolyl) aminomethylenemalonates gave ethyl 6, 9-dihydro-9-oxo-3H-pyrrolo [4, 3-f] quinoline-8-carboxylates, alkylation of which followed by hydrolysis afforded a variety of N6-substituted pyrazolo [4, 3-f] quinoline-8-carboxylic acids in a good yield. N6-Substituted pyrazolo [3, 4-f] quinoline-8-carboxylic acids were also obtained from ethyl 6, 9-dihydro-9-oxo-1H-pyrazolo [3, 4-f] quinoline-8-carboxylate in the same way. These compounds were synthesized in order to evaluate their antimicrobial activity.
  • 中島 憲一郎, 中野 洋子, 秋山 修三
    1978 年 98 巻 8 号 p. 1072-1076
    発行日: 1978/08/25
    公開日: 2008/05/30
    ジャーナル フリー
    Coloration of some metal ions with 6-amino-5-nitroso-1, 2, 3, 4-tetrahydropyrimidine derivatives and the detection limit of these metal ions were examined. Among metal ions such as Co (II), Cu (II), Ni (II), Fe (II), cobalt and iron show especially sensitive color change. Therefore further examination was made to utilize these reagents for the spectrophotometric determination of cobalt and iron. Among these reagents, 6-amino-1-methyl-5-nitroso-4-oxo-2-thioxo-1, 2, 3, 4-tetrahydropyrimidine (VI) and 6-amino-5-nitroso-4-oxo-1-phenyl-2-thioxo-1, 2, 3, 4-tetrahydropyrimidine (VIII) were the most sensitive reagents for cobalt and iron. The molar absorption coefficients of the complexs of VI and VIII with cobalt are 4.72×104 and 4.48×104 l·mol-1 cm-1, those with iron are 2.37×104 and 2.51×104 l·mol-1 cm-1, respectively. Dissociation constants (pK) of these reagents were determined by spectrophotometry. Generally, pK values of the reagents containing thioxo group were smaller than those of the corresponding reagents.
  • 中島 憲一郎, 中野 洋子, 秋山 修三
    1978 年 98 巻 8 号 p. 1077-1080
    発行日: 1978/08/25
    公開日: 2008/05/30
    ジャーナル フリー
    Spectrophotometric determination of iron (II) with 6-amino-5-nitroso-4-oxo-1-phenyl-2-thioxo-1, 2, 3, 4-tetrahydropyrimidine (ANTP) was investigated. ANTP reacts with iron (II) to form a water-soluble blue complex. Iron (II)-ANTP complex has an absorption maximum at 670 nm against the reagent blank and shows a definite absorbance over a range of pH 5.5 to 8.5. Beer's law holds over a range of 0-20 μg/10 ml of iron (II) at 670 nm, the molar absorption coefficient of the complex is 2.5×104 l·mol-1 cm-1. Ni (II), Hg (II), Co (II), Pb (II), Bi (III), and citrate interfered with this determination. The molar ration of iron (II) to ANTP in the complex was 1 : 3. The established procedure is as follows : To a sample solution, 1 ml of 1% hydroxylamine hydrochloride solution, 2 ml of acetate buffer (pH 6), 2 ml of 0.01 M ANTP-dimethylformamide solution, and 1 ml of EtOH are added. The mixture is diluted to 10 ml with water, and its absorbance is measured at 670 nm against the reagent blank. This method was successfully applied to commercial tablets and capsule of iron (II).
  • 濱田 喜樹, 杉浦 道治
    1978 年 98 巻 8 号 p. 1081-1091
    発行日: 1978/08/25
    公開日: 2008/05/30
    ジャーナル フリー
    Application of cyanogen bromide to benzo [f] quinoline (3) in methanol, followed by the application of bromine and sodium carbonate gave 2-bromo-4-cyano-1, 3-dimethyl-1, 2, 3, 4-tetrahydrobenzo [f] quinoline (8a) in a good yield. Hydrolysis of 8 resulted in quantitative formation of 2-bromobenzo [f] quinoline (5), which formed 2-aminobenzo [f]-quinoline (9) by the action of potassium amide in liquid ammonia or 3 by the action of stannous chloride. 2-Bromo-3-cyanobenzo [f] quinoline (15), obtained from 2-bromobenzo-[f] quinoline N-oxide (16) by the application of benzoyl chloride in the presence of potassium cyanide, formed 2-bromo-3-cyanobenzo [f] quinoline (19) by the action of stannous chloride. 19 was also obtained by the application of benzoyl chloride to benzo [f] quinoline N-oxide (17) in the presence of potassium cyanide. 15 was also obtained by debenzoylation of 4-benzoyl-2-bromo-3-cyano-3, 4-dihydrobenzo [f] quinoline (14). Examination of the reactivity of 8a indicated that methanol and water easily added to the N-C≡N group in the presence of a base. Benzo [h] quinoline (4) was similarly derived to 3, x-dibromobenzo [h] quinoline (21) via 3, x-dibromo-1-cyano-2, 4-dimethoxy-1, 2, 3, 4-tetrahydrobenzo [h] quinoline (20) and its hydrolysis. Application of cyanogen bromide to 4, 6-phenanthroline (22) in methanol gave 6-cyano-5-methoxy-5, 6-dihydro-4, 6-phenanthroline (23), and 2-bromo-4, 6-phenanthroline (24) could not be obtained.
  • 渡辺 康, 佐野 倫男, 本橋 清, 米田 良蔵
    1978 年 98 巻 8 号 p. 1092-1100
    発行日: 1978/08/25
    公開日: 2008/05/30
    ジャーナル フリー
    Since of bioavailability of enteric-coated commercial erythormycin tablets seemed to depend on dissolving pH of the enteric coating agents, examinations were made on specially prepared tablets coated with enteric-coating agents having different dissolving pH ranging from 5.0 to 7.0. After confirming disintegration and dissolution characteristics of the tablets, the preparations were administered to more than 10 healthy volunteers, and serum levels of the drug and area under the serum level vs time curve (AUC) were determined. Significant differences were found in the results obtained from tablets coated with the agents having dissolving pH of 5.0 or 5.5 and those of pH 6.5 or 7.0. These results supported the previous findings and might present important information to formulators in selecting enteric coating agents to formulate more effective preparations with high bioavailability. During the couse of the study, relation of the bioavailability to coefficients of variation in the maximum serum level of the drug and in the AUC were also investigated in detail, and it was found that the coefficients of variation were small when the tablets having high bioavailability were adminstered.
  • 浜島 良, 岩野 勝行, 奥田 博久
    1978 年 98 巻 8 号 p. 1101-1107
    発行日: 1978/08/25
    公開日: 2008/05/30
    ジャーナル フリー
    A new series of guaiazulenylglyoxylamides, guaiazulenylglyoxylic acid esters, guaiazulenylglyoxylpiperazines, and acylaminoguaiazulenes was prepared, and their antiallergic activity was evaluated by passive cutaneous anaphylaxis (PCA) test in the rat. Guaiazulenylglyoxalyl chloride derived from guaiazulene using oxalyl chloride was converted into glyoxylamides (1), glyoxylic acid esters (2), and glyoxylpiperazines (3) with appropriate amine, alcohol, or piperazine, respectively. Acylaminoguaiazulenes (5) were prepared from 3-aminoguaiazulene with the corresponding acid anhydride or acid chloride. Many of guaiazulenylglyoxylpiperazines (3), particularly some of them (3a, 3b, 3c, 3f and 3g) showed a high antiallergic activity by oral administration, but none of the other types of compound showed a significant activity.
  • 浜島 良, 岩野 勝行, 奥田 博久
    1978 年 98 巻 8 号 p. 1108-1113
    発行日: 1978/08/25
    公開日: 2008/05/30
    ジャーナル フリー
    Based on previous work that many of guaiazulenylglyoxylpiperazines possess an antiallergic activity, series of p-(3-guaiazulenyl azo) benzenesulfonamides (1-18) were prepared and their antiallergic activity was evaluated by passive cutaneous anaphylaxis (PCA) test in the rat. The compounds were prepared by treating guaiazulene with diazonium salts of sulfonamides in the presence of sodium acetate. Many of them showed a high antiallergic activity on oral administration. The structure-activity relationship for guaiazulene derivatives was discussed.
  • 尾藤 龍哉, 工藤 正典, 奥村 誠
    1978 年 98 巻 8 号 p. 1114-1118
    発行日: 1978/08/25
    公開日: 2008/05/30
    ジャーナル フリー
    Gastrointestinal damage induced by oral administration of non-steroidal anti-inflammatory agents (flurbiprofen, phenylbutazone, indomethacin and acetylsalicylic acid) was examined. Wistar male rats in the fasting state for 16 hr received the agent orally. The dose was determined from the equation : D=ED50×Ki, where ED50 is the median inhibitory dose on carrageenin edema in the rat paw and Ki is the coefficient. Rats were sacrificed without anesthesia by cutting both common carotid arteries at 6 hr after drug administration and gastrointestinal specimens were visually examined. Cumulative method was applied for statistical analysis of the number of rats in each grade of the damage. On the basis of the statistical data, availability (balance between effect and hazard) of the tested drugs was discussed, and safety margins and their confidence limits at ED50 of the drugs were estimated. In further study, median ulcerogenic dose (UD50) and UD50/ED50 ratio of each tested drug were calculated. Availability evaluated on statistical consideration decreased in the order of flurbiprofen, phenylbutazone, indomethacin, and acetylsalicylic acid.
  • 篠田 雅人, 清水 素行, 中陳 静男, 櫛 泰典, 鴨川 旭
    1978 年 98 巻 8 号 p. 1119-1122
    発行日: 1978/08/25
    公開日: 2008/05/30
    ジャーナル フリー
    Intravenous injection of parotin to rabbits results in leukocytopenia from 5 min after the injection, and a biphasic change of decrease to 10 min and increase after 20 min of injection was observed. This leukocytosis-promoting activity of parotin was also observed in splenectomized, adrenalectomized, or nephrectomized rabbits. This leukocytosis-promoting activity of parotin was found to appear even in rabbits with liver damage due to carbon tetrachloride.
  • 大平 健一, 有吉 敏彦
    1978 年 98 巻 8 号 p. 1123-1128
    発行日: 1978/08/25
    公開日: 2008/05/30
    ジャーナル フリー
    Essential metal contents and the liver microsomal drug-metabolizing enzyme systems were investigated in rats treated simultaneously with cadmium chloride and lead acetate. 1) Calcium content in the liver increased markedly both by a single injection of lead acetate and by simultaneous administration of lead acetate and cadmium chloride, and this increase was observed even after 30 days. 2) Zinc content in the liver increased significantly 1 and 3 days for male rats and 3 days for female rats after a single administration of cadmium chloride. Copper content in the kidney also increased 15 days after the injection of cadmium chloride. 3) Content of δ-aminolevulinic acid in urine increased markedly by the injection of lead acetate and this increase was still noted after 30 days. 4) Of the fatty acid constituents in the liver phospholipids, stearic acid increased, and arachidonic acid decreased by the treatment with cadmium chloride and/or lead acetate. 5) Content of cytochrome P-450 and b5, as well as activities of aniline hydroxylase and aminopyrine demethylase, decreased markedly by the administration of cadmium chloride and/or lead acetate.
  • 吉沢 逸雄, 藤間 貞彦, 木村 道也
    1978 年 98 巻 8 号 p. 1129-1131
    発行日: 1978/08/25
    公開日: 2008/05/30
    ジャーナル フリー
    A nucleoside, thymidine, was isolated and identified from the dried flowers of lily of the valley, Convallaria keisukei MIQ., growing in Hokkaido.
  • 檜山 千都子, 宮井 幸枝, 吉田 久信, 山崎 和男, 田中 治
    1978 年 98 巻 8 号 p. 1132-1137
    発行日: 1978/08/25
    公開日: 2008/05/30
    ジャーナル フリー
    Uracil was isolated and identified, from the crude drug, white Ginseng (main roots of Panax ginseng), while guanine and adenine were isolated and identified from fibrous side roots (root-hair) of this plant. Beside these bases, high-speed liquid chromatography on TSK-gel LS 160 (solvent : H2O only) and on G-3000 W and G-2000SW (solvent : H2O : AcOH : Et3N=100 : 0.3 : 0.3) indicated the presence of uridine, guanine, and adenine in the white ginseng, and uridine, uracil, and adenosine in the root-hair. Quantitative analysis of these bases and nucleosides in this crude drug by dual-wavelength thin-layer chromatography-densitometry is also described.
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