Structural analysis was made on the glucan moiety of purified antitumor active polysaccharide fraction H
11 obtained from the mycelia of
P. cocos.
In the
13C-nuclear magnetic resonance spectrum of fraction H
11, signals at 102.9 and 84.8ppm were observed, and assigned to C-1 of the β-linkage and C-3 of the β-D-(1, 3)-linked D-glucosyl redidues, respectively. On addition of NaOD solution, the C-1 signal was separated into two signals at 104.2 and 104.8ppm which can be assigned to the C-1 signal of the β-D-(1, 3)-linked and the β-D-(1, 6)-linked D-glucosyl residues, respectively.
The molecular weight of fraction H
11 was calculated to be approx. 5×10
6 by the gel filtration method with CPG-1000Å.
Complete methylation and hydrolysis of fraction H
11 gave 2, 4, 6-tri-, 2, 3, 4-tri-, 2, 4-di-, and 2, 3, 4, 6-tetra-
0-methyl glucose as alditol acetates in a molar ratio of 3.96: 0.95: 1.05: 1.00.
From these results, it has been revealed that fraction H11 is a highly branched (1, 3)-(1, 6)-β-D-glucan containing each component in 4: 1 ratio.
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