The distribution of cytochrome c heme octapeptide (CHP) was investigated by the tacer technique and whole body autoradiography. Purified CHP from yeast cytochrome c was tritiated by Wilzbach's method and purified by chromatography on Amberlite IRC-50 column, Preparation of
35S-labeled CHP was performed by tryptic and peptic digestion of
35S-cytochrome c which was obtained by cultivation of yeast, Candida krusei, in the medium containing H
235SO
4. Radioactivity was clearly observed in most of the organs 1 hr after the intravenous and subcutaneous injection of
3H-CHP in the rats. A relatively high uptake of.radioactivity was seen in the blood, kidney, lung, liver, and heart. The radioactivities in the brain, however, increased markedly much slowly compared with those in other tissues, and reached a maximum after 3 days. In contrast the orally administered
3H-CHP was scarcely incorporated through the intestine, and 47% of the radioactivity still remained in the gastro-intestinal tract after 7 days.
35S-CHP also appeared to be absorbed, distributed, and excluded similarly as
3H-CHP regardless of the way of injection. Whole-body autoradiography using
35S-CHP in the mice gave almost the same results as above. It is of interest that, different from the metabolism of cytochrome c and amino acids such as cystine and phenylalanine, CHP is readily incorporated and remained in the tissues, especially hippocamps in the brain, secretory glands such as masal and salivary glands, and the white pulp in the spleen.
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