Examinations were made on the determination of blood level of isonicotinoylhydrazinopyruvic acid (IP) and free pyruvic acid, and excretion of vitamin B
6 in urine, which are problems to be solved in the clinical use of IP as an antituberculosis agent, having the same effect as INAH with less toxicity. From the result of various experiments, following conclusions were drawn:
1) Comparison of paper chromatographic and ion exchanger methods for separatory determination of IP in blood showed that the two methods give well-agreeing values. Paper chromatographic method was found to be more practicable.
2) For the determination of free pyruvic acid in blood after administration of IP, total pyruvic acid, which included the bound form, was measured by the 2, 4-dinitrophenylhydrazine method, and the amount of the free acid was calculated by subtracting the value of pyruvic acid so obtained from IP.
3) The foregoing processes were used to determine IP and free pyruvic acid in blood after administration of IP, and it was found that:
a) Blood IP disappeared rapidly after intravenous injection of IP-Na in a rabbit and it was not detected after 2 hours.
b) After oral administration of IP-Na in a rabbit, blood level of INAH increased gradually, reached the maximum after about 2 hours, and the high level was maintained for 4-5hours.
c) In either of the cases, the amount of free pyruvic acid in blood did not show any increase.
4) Excretion of vitamin B
6 in urine clearly increased after administration of IP and showed results similar to that after administration of INAH.
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