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Volume 16, Issue 4
Displaying 1-11 of 11 articles from this issue
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Effect of Tolbutamide on Gluconeogenesis and Ketogenesis in the Isolated Perfused Rat LiversNobuo Sakamoto, Nigishi Hotta, Masayoshi Yoshida, Yuzo Sato, Makoto Na ...1973 Volume 16 Issue 4 Pages 275-280
Published: July 31, 1973
Released on J-STAGE: August 10, 2011
JOURNAL FREE ACCESSDownload PDF (954K) -
Reiko Saito, Yoko Kashimoto, Reiko Odagiri, Yasuo Akanuma, Kinori Kosa ...1973 Volume 16 Issue 4 Pages 281-289
Published: July 31, 1973
Released on J-STAGE: August 10, 2011
JOURNAL FREE ACCESSThe serum lecithin-cholesterol acyltransferase (LCAT) esterifies circulating cholesterol. Recently positive correlation of serum LCAT activity with relative body weight has been reported, however, the relationship between the change of body weight and serum LCAT activity has not been clarified.
The present study was designed to elucidate the relationship between serum LCAT activity and serum lipids after the change of body weight in obese and lean subjects.
The results are as follows.
(A) Measurement of LCAT activity
1. As the substrate for the LCAT assay plasma d>1.063 fraction (HDL) is preferable to whole plasma.
2. The reaction of cholesterol esterification using that substrate plasma proceeded linearly for 6 hours incubation. There was also linear relationship between it's reaction and the LCAT concentration up to 40μl/ml medium.
3. Unesterified cholesterol in the incubation medium did not affect on the assay system, if LCAT activity was expressed as μg/ml/hr.
4. When the serum sample was frozen and maintained at-15°C until the LCAT assay, the activity of LCAT did not decrease at least for 16 weeks.
(B) The LCAT activity and serum lipids were measured in nine obese and four lean subjects before and after the change of body weight
1. The LCAT activity in obese subjects decreased significantly together with weight loss by diet restriction, while it's activity increased following weight gain in lean subjects by high calorie diet. The serum LCAT activity was found to correlate with the relative body weight before and after the change of body weight. There was also a significant positive correlation between serum LCAT activity and the change of body weight.
2. Serum total cholesterol and cholesterol ester in obese subjects became significantly lower following weight loss by diet restriction. Serum triglyceride and serum unesterified cholesterol in obese subjects decreased after weight reduction and they increased mostly after weight gain in lean subjects, though it was not significant.View full abstractDownload PDF (1269K) -
The Relation between Causes of Death and Therapeutic Methods of DiabetesYukimasa Hirata, Toshihiko Mihara, Katsushi Sakasegawa, Yoshihiro Ando ...1973 Volume 16 Issue 4 Pages 290-299
Published: July 31, 1973
Released on J-STAGE: August 10, 2011
JOURNAL FREE ACCESSA retrospective study of causes of death in 1, 885 diabetic patients treated in 384 hospitals and died during 1968-70 in Japan is reported.
Significant differences of death causes were observed between the groups of the patients divided by the duration of diabetes (under 5 years or over 5 years), also among the groups divided by therapeutic methods of diabetes (diet alone, oral hypoglycemic agents or insulin).
Concerning angiopathies, the incidence of deaths due to nephropathy and ischemic heart disease (IHD) was higher in the group of patients suffering from diabetes over 5 years. An incidence of cerebrovascular disease as a cause of death was not so different between the two groups divided by the duration of 5 years. A significant difference in the cause of death was also shown among the groups treated with diet only, oral hypoglycemic agents or insulin. The principal causes of death were diabetic coma and diabetic nephropathy for the insulin-treated group, cerebrovascular disease and IHD for the tablet-treated group, and malignant neoplasms for the diet-treated group.
In particular, it was noteworthy in this study that an incidence of death cases due to IHD was significantly higher in the tablet-treated group compaired with other groups. However, this phenomenon trended already within 3 years after the initiation of the drug therapy. Therefore there would be an apparent association between IHD and oral hypoglycemic therapy.View full abstractDownload PDF (2064K) -
Hiroshi Mabuchi, Hiroshi Maki, Shyuichi Shirasaki, Kazuya Saito, Tomoo ...1973 Volume 16 Issue 4 Pages 300-307
Published: July 31, 1973
Released on J-STAGE: August 10, 2011
JOURNAL FREE ACCESSTwenty-five patients with hyperthyroidism were classified into the three groups; normal (8), borderline (10) and diabetic ones according to the diagnostic criteria for diabetes, proposed by the Japan Diabetic Association. Blood glucose, FFA and IRI levels before and after the oral administration of 50 g of glucose in each of the thyrotoxic groups were compared with those obtained from 10 control subjects. The results were as follows.
1. In the thyrotoxic patients the blood glucose levels were significantly higher than those in the control group at 30, 60, 120 minutes after glucose loading. Even in the group of hyperthyroidism with normal glucose tolerance, the level of blood glucose was significantly increased at 30 minutes. Nine cases of 10 borderline subjects showed oxyhyperglycemia in the glucose tolerance test.
2. The plasma levels of IRI in hyperthyroidism were significantly elevated before and at 120, and 180 minutes after glucose loading. There was a tendency indicating that to some extent the severer the impairment of glucose tolerance, the higher the plasma IRI response to glucose loading. In patients with much more severe impairment in glucose tolerance, the response of plasma IRI to the glucose showed slightly lower and delayed pattern.
3. The fasting levels of FFA in hyperthyroidism were higher than those of control group and promptly decreased with a subsequent reboud at 180 minutes in response to glucose loading. In thyrotoxic patients showing diabetic glucose tolerance curve, serum FFA tended to fall more slowly and never returned to its initial level at 180 minutes.
Possible mechanism of glucose intolerance in hyperthyroidism was discussed.View full abstractDownload PDF (1429K) -
Takayoshi Toyota, Mikihiko Kudo, Hiroaki Kikuchi, Yoshio Goto1973 Volume 16 Issue 4 Pages 308-313
Published: July 31, 1973
Released on J-STAGE: August 10, 2011
JOURNAL FREE ACCESSThis study was performed to see the insulin secretion from the perfused pancreas of streptozotocin (STZ) diabetetic rats. Mild (DM-1), moderate (DM-2) and severe (DM-3) diabetic rats were made by single injection of STZ 20, 30 and 40 mg per kg body weight intravenously and used for the perfusion experiment 7 days after the injection. DM-2 group was the rats used for the experiment 28 days after the 30 mg/kg STZ injection. OGTT (1.5 g/kg) was done before the perfusion of the pancreas. The perfusion was carried out by the modified method of Grodsky. Insulin secretion of normal rats induced by 300 mg% glucose showed a diphasic curve. However, those of DM-1, DM-2 and DM-3 were monophasic. Insulin secretion area of DM-1 group during 60 minutes decreased to about 7% of that of normal rats. This result revealed that the decrease of the insulin secretion was related with glucose intolerance. The insulin secretion area of DM-2, DM-2 and DM-3 group decreased to about 45%, 78% and 95% of normal rats, respectively. Insulin like activity (ILA) of the perfusate estimated by the method of Martin at 3 and 60 minutes during the perfusion of the pancreas by 300 mg% glucose was proportional to immunoreactive insulin (IRI).
Conclusively, the decrease of insulin secretion area to about 7-45% of normal rats caused the glucose intolerance although the fasting blood sugar was normal. The decrease of that to over 78% of normal rats produced an elevation of the fasting blood sugar.View full abstractDownload PDF (2614K) -
Correlation between Plasma Immunoreactive Insulin and Erythrocyte G-6-PD ActivityToshihiko Mihara, Shoshi Shimoyama, Sekiji Morita, Isao Matsuoka, Hiro ...1973 Volume 16 Issue 4 Pages 314-319
Published: July 31, 1973
Released on J-STAGE: August 10, 2011
JOURNAL FREE ACCESSBlood sugar, plasma immunoreactive insulin (IRI) and erythrocyte glucose-6-phosphate dehydrogenase (G-6-PD) activity were determined in 25 nontreated patients with glycosuria during an oral glucose tolerance test. The erythrocyte G-6-PD activity seemed to be lower in the group with a diabetic pattern of blood sugar response than in the group with a normal pattern in the glucose tolerance test. However, statistically a difference of the G-6-PD activity between two groups, normal and diabetic, was not significant. A low erythrocyte G-6-PD activity was shown in the group with low plasma IRI response and/or low Δplasma IRI/Δplood sugar ratio after oral administration of 50g glucose. Any correlation between fasting blood sugar levels and fasting erythrocyte G-6-PD activity was not shown statistically and a correlation coefficient “r” was 0.0951 (p>0.05, N=25). On the other hand, a positive correlation between fasting erythrocyte G-6-PD activity and each of plasma IRI levels at 30 minutes after oral glucose load and of Δplasma IRI/Δplood sugar ratio was observed and an individual correlation coefficient “r” was 0.4431 (p<0.05, N=25) and 0.5496 (p<0.01, N=23), respectively.View full abstractDownload PDF (1069K) -
A Case Report of Acute Onset and Dramatical Remission of Young Diabetic with AcromegalyNobuo Sakamoto, Nigishi Hotta, Makoto Nagashima, Akihisa Iguchi, Hiron ...1973 Volume 16 Issue 4 Pages 320-328
Published: July 31, 1973
Released on J-STAGE: August 10, 2011
JOURNAL FREE ACCESSIt is well known that diabetes mellitus is more common in acromegalic patient than in the general population. Kozak (1971) reports on an incidence of diabetes of the patients among his collected series of 823 cases of acromegaly. The question then arises as to why only some of acromegalic subjects develop diabetes. This problem, however, still remains conflicting.
This paper reports a case of acute onset and dramatical remission of young diabetic with acromegaly. A woman, aged 17 years at onset of diabetes in November, 1972, was first seen at our clinic when in diabetic pre-come. The partial operation was performed under the diagnosis of the pituitary adenoma and irradiation (4670 rad.) was given in August, 1972. Subsequently, over a period of operation and irradiation, thirst, polyuria and glucosuria occured. She required 80 units of regular insulin daily at maximum. In three months with insulin treatment, it resulted surprisingly in satisfactory control without requirement of insulin. Good diabetic control over a year is maintained without insulin administrations.
The changes of glucose, free fatty acid, lactate, pyruvate, ketone body, IRI and HGH in serum were observed all over the progress of onset and remission of diabetes in this case. The possibility that insulin and growth hormone play a regulatory role in energy homeostasis is suggested by the observation that the occurrence of diabetes, obesity, hypoglycemia or metabolic disorders depend on the interrelationship between metabolites and hormones.View full abstractDownload PDF (4206K) -
Shuhei Tsuboi, Masanobu Imai, Takashi Tanigawa, Terukatsu Arima, Takeh ...1973 Volume 16 Issue 4 Pages 329-336
Published: July 31, 1973
Released on J-STAGE: August 10, 2011
JOURNAL FREE ACCESSPyogenic spondylitis is a rare disease. A little over 100 cases have been previously reported in Japan, only one of these cases was associated with diabetes mellitus. This is a report of typical pyogenic spondylitis which was associated with a history of diabetes mellitus for 20 years.
In September 7, 1971, this 56 years old male, a president of a company, was admitted to our hospital with a complaint of severe neuralgic pain in the both hands and feet for 3 years. In Octover 22, after playing golf, he complained of lumbago, in addition to the foregoing pain. A week later, the pain spread in the hip, inguinal and thigh areas. At the same time, he was attacked with a chill and a fever of 39°C which continued for only 2 days, and constipated. The laboratory data showed an accelerated BSR and a positive CRP. The other clinical examinations were also performed, but a correct diagnosis was unabled to make.
Two months after onset of the symptoms, the X-ray films of the vertebrae showed erosions of the contiguous borders of the second and third lumbar vertebrae. Accordingly a diagnosis of pyogenic spondylitis was made. Immobilisation on a plaster bed was begun and antibiotics were administerd (PC, CEX, etc.). Four months later, the X-ray films of the vertebrae showed osteolar bridges and calcification. The laboratory data improved. Through his whole course, 4, 000 mg of pentazocine was intravenously administered as an analgesic against pain. Diabetes mellitus was controlled with semilente insulin 40 units daily.
Our case was compared with those of previously reported in the literature with emphasis of diagnosis and therapy.View full abstractDownload PDF (6932K) -
Taizo Hayashi, Shinichiro Nagai, Yoshikiyo Misugi, Haruo Yamaguchi, Sh ...1973 Volume 16 Issue 4 Pages 337-340
Published: July 31, 1973
Released on J-STAGE: August 10, 2011
JOURNAL FREE ACCESSL-leucine loading tests were carried out in a case of 65 years female patient with insulinoma and 5 normal subjects. For the patient the tests were performed before and after the surgical treatment. The changes of leucine, other amino acids, glucose and IRI in blood were investigated during L-leucine loading test.
asting blood amino acid levels were generally lower in the case of insulinoma than in normal subjects. After the treatment, however, these concentrations increased and indicated normal or high values.
Blood concentrations of leucine showed the most significant changes as compared with other amino acids during L-leucine loading test. In normal subjects blood leucine levels elevated to 615 mp Mol/ml in means, without the changes in blood glucose and plasma IRI. On the other hand this elevation in the case of insulinoma reached to remarkable high value, 2290 mμ Mol/ml, and then the significant decrease in blood sugar and significant increase in plasma IRI were observed with a cramp and coma. After treatment the elevation of leucine levels was still observed in high concentration, 2370 mp Mol/ml. plasma IRI also increased, but no change in blood was found.
Namely a relationship between insulin relaese and blood leucine level was demonstrated in our cases during L-leucine loading test. It may be suggested that the rise of blood leucine concentration by the administration gives some effects on insulin release, but the further examination must be done to clarify the relation between leucine metabolism and insulin release.View full abstractDownload PDF (693K) -
1973 Volume 16 Issue 4 Pages 341-357
Published: July 31, 1973
Released on J-STAGE: August 10, 2011
JOURNAL FREE ACCESSDownload PDF (3923K) -
1973 Volume 16 Issue 4 Pages 359-373
Published: July 31, 1973
Released on J-STAGE: August 10, 2011
JOURNAL FREE ACCESSDownload PDF (3695K)
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