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49 巻 , 3 号
選択された号の論文の6件中1~6を表示しています
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論文
  • Chamilani Nikapitiya, Sung-Ju Jung, Myung-Hwa Jung, Jun-Young Song, Je ...
    49 巻 (2014) 3 号 p. 93-112
    公開日: 2014/10/02
    ジャーナル フリー
    We identified and characterized full-length cDNAs of rock bream Oplegnathus fasciatus major histocompatibility complex (RbMHC) class I and β2-microglobulin (Rbβ2m) molecules. The full-length RbMHC class I cDNA was 2,667 bp with a 1,227 bp open reading frame encoding 409 amino acids (aa). The Rbβ2m full-length cDNA (1,339 bp) consisted of a 354 bp coding region, encoding 118 aa. RbMHC class Iα was clustered with the Z/ZE lineage of known MHC class I sequences, suggesting a novel molecule belonging to the Z/ZE lineage. Although characteristic features (residues and motifs) known to be conserved in classical and non-classical class I molecules were conserved in RbMHC class Iα with few modifications, it is more closely resembled to classical MHC class I. RbMHC class I and Rbβ2m were transcribed constitutively in healthy fish, but expression differed among the different organs and was highest in whole blood. Low sequence variation in the RbMHC class I α1 region makes it uncertain whether the molecule belongs to the non-classical group. However, because transcription was induced by megalocytivirus, the molecule is most likely a member of the classical group. Our results provide important information for future studies on antigen presentation and cytotoxic CD8+ T cell responses in rock bream against infectious pathogens.
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  • Zhenxing Liu, Hao Ke, Yanping Ma, Le Hao, Guoqing Feng, Jiangyao Ma, Z ...
    49 巻 (2014) 3 号 p. 113-120
    公開日: 2014/10/02
    ジャーナル フリー
    Cyprinid herpesvirus 3 (CyHV-3), also called as koi herpesvirus, has spread all over the world since its emergence in the late 1990s. CyHV-3 causes a fatal disease, which leads to a mortality rate of more than 80% in common and ornamental (koi) carp Cyprinus carpio. The novel anti-CyHV-3 strategies are urgently needed. In this report, CyHV-3 particles were concentrated and then used for the immunization of hens in order to obtain the anti-CyHV-3 egg yolk immunoglobulin (IgY). The proportion of anti-CyHV-3 IgY to total IgY in water-soluble fraction of egg york increased from 0.014% to 23.9% after three times of immunization. The anti-CyHV-3 effects of IgY were then examined by in vivo and in vitro assays. The results suggested that the anti-CyHV-3 serum of hens and the purified anti-CyHV-3 IgY from eggs could prevent common carp brain (CCB) cells from infection. After oral passive immunization with IgY, the mortality of carp significantly decreased from 85% to 50% (P < 0.05) when the fish were challenged with CyHV-3 at a dose of 40 TCID50/fish. These results will possibly pave the way for the prevention of CyHV-3 infection.
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  • Tanvir Rahman, Koushirou Suga, Kinya Kanai, Yukitaka Sugihara
    49 巻 (2014) 3 号 p. 121-129
    公開日: 2014/10/02
    ジャーナル フリー
    Tenacibaculum maritimum is a Gram-negative, gliding marine bacterium that causes tenacibaculosis, an ulcerative disease of marine fish. The bacterium usually forms rhizoid colonies on agar media. We isolated T. maritimum that formed slightly yellowish round compact colonies together with the usual rhizoid colonies from a puffer fish Takifugu rubripes suffering from tenacibaculosis, and studied the biological and serological characteristics of a representative isolate of the compact colony phenotype, designated strain NUF1129. The strain was non-gliding and avirulent in Japanese flounder Paralichthys olivaceus in immersion challenge test and showed lower adhesion ability to glass wall in shaking broth culture and to the body surface of flounder. It lacked a cell-surface antigen commonly detected in gliding strains of the bacterium in gel immunodiffusion tests. SDS-PAGE analysis showed different polypeptide banding patterns between NUF1129 and gliding strains. Like gliding strains, NUF1129 exhibited both chondroitinase and gelatinase activities, which are potential virulence factors of the bacterium. These results suggest that some cell-surface components related to gliding and adhesion ability are implicated in the virulence of T. maritimum.
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  • 南 隆之, 岩田 一夫, 桑原 正和, 天野 健一, 水田 篤, 山下 亜純, 福田 穣, 西木 一生, 津江 佑哉, 吉田 照豊
    49 巻 (2014) 3 号 p. 130-136
    公開日: 2014/10/02
    ジャーナル フリー
     先の研究でカワハギに対するS. iniae市販ワクチン,L. garvieae市販ワクチン,およびこれらの2種混合ワクチンの効果について評価した。本研究では,これら3種類のワクチンについて,効果持続性,地理的に由来が異なる攻撃株に対する効果および免疫期間中の水温の影響を検討した。その結果,これらのワクチンは異なる種類のS. iniaeおよびL. garvieae攻撃株に対して強い防御効果を誘導し,その効果は接種52週間後まで確認された。また,これらのワクチンは,水温15°C,20°Cおよび25°Cで接種されたカワハギに防御効果を誘導した。
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