In this study, we assessed the influence of different water temperatures on the swimming ability of the oncomiracidium of Neobenedenia girellae. In addition, we investigated the temporal change in their infectivity to Seriola dumerili after hatching at 25°C. Most oncomiracidia in the cell culture flask filled with seawater deciliated and predominantly crept on the bottom by the end of 6 h experiment. Approximately 9% of the oncomiracidia in seawater-containing wells of a 12-well cell culture plate were non-swimming and became trapped in the air-water surface interface. However, these effects were mitigated when a polyethylene sheet was placed on the seawater surface in the wells. When conducting the experiment using this method, the oncomiracidia remained active and were still swimming at 60, 72, and 108 h after hatching at 30°C, 25°C, and 20°C, respectively. The retention time of the oncomiracidia's swimming ability decreased as the water temperature increased. The oncomiracidial infectivity was reduced after 24–25.5 h or more after hatching. At 5 days after the exposure to S. dumerili, the number of attached N. girellae was significantly lower, and their body length was significantly shorter when using oncomiracidia that were 36–37.5 h after hatching compared to freshly hatched ones.
Flavobacterium psychrophilum can infect rainbow trout Oncorhynchus mykiss and ayu Plecoglossus altivelis, detrimentally affecting global aquaculture. This study aimed to understand the infectivity and host specificity of two F. psychrophilum strains, SG950607 and PH0424, which were isolated from the kidneys of rainbow trout and ayu, respectively. Rainbow trout (fry) and ayu (adult and fry) were infected via two methods: bath immersion and intraperitoneal injection. We investigated the ability of each F. psychrophilum strain to survive in the serum of non-original host fish species. Rainbow trout died upon being infected by PH0424. In addition, the complement of the rainbow trout did not show a bactericidal effect against PH0424. On the other hand, ayu infected with SG950607 survived, and their complement eliminated this strain. The difference in mortality rates is likely associated with the susceptibility of each strain to the complement activity possessed by the host fish.
ニジマス養殖における伝染性造血器壊死症(IHN)の被害抑制を目的として,1989年に分離されたIHNウイルス(IHNV)TK8901株を用いた人為感染実験による選抜育種を行った。感染実験の累積死亡率は選抜育種により減少し,第8世代の選抜群は5%以下であった。そこで,2016年に東京都内で認められた病魚から分離されたIHNV株を用いて第9世代に対し感染実験を行ったところ,全てのウイルス株において,対照の非選抜群と比較して有意に低い累積死亡率が認められた。これらの結果は,選抜第8世代以降の交配群はIHN耐性ニジマスとして,IHNVに対する高い抵抗性を獲得したことを示している。
Susceptibilities of three hybrids between crucian carp including Carassius cuvieri (Ccu), Carassius buergeri grandoculis (Cbg) and Carassius buergeri subsp.1 (Cbs) and common carp Cyprinus carpio (Cc) to KHV were evaluated in an infection experiment. Infection rates of KHV in each hybrid were 30% and 20% in Ccu × Cc, 40% and 30% in Cbg × Cc, and 10% and 0% in Cbs × Cc, at 14 and 44 dpe, respectively. This indicates that Cbs × Cc shows lower sensitivity to KHV than the other two hybrids. More importantly, Ccu × Cc and Cbg × Cc transmitted the virus to co-habited naïve koi.