魚病研究
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49 巻 , 1 号
選択された号の論文の5件中1~5を表示しています
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論文
  • 山下 亜純, 武智 昭彦, 高木 修作, 佐藤 彩乃
    49 巻 (2014) 1 号 p. 1-6
    公開日: 2014/03/25
    ジャーナル フリー
    ホスホマイシン(FOM)のマダイエドワジエラ症に対する治療効果を実験感染魚ならびに自然感染魚で調べた。愛媛県内の養殖マダイから分離されたE. tarda 67株に対するFOMの最小発育阻止濃度は 1~4 μg/mLであり,本剤に対する耐性株は確認されなかった。実験感染魚では,FOM 10~80 mg/kg/dayの範囲では投薬量の増加に伴い死亡率が低下し,FOM 40~80 mg/kg/day 6 日間の投薬により有意な治療効果が認められた。自然発症した死亡率の異なる養殖場のマダイにFOM 40 mg/kg/day を 6 日間投薬した結果,FOM投薬群の累積死亡率はいずれも無投薬群に比べ有意に低下した。また,投薬開始時の日間死亡率が低い養殖場の方が,投薬後の死亡率が低く推移した。これらのことから,FOMはマダイのエドワジエラ症治療薬として有効であり,効果を高めるためには出来るだけ早期の投薬が重要であることが明らかとなった。
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  • Shinpei Wada, Osamu Kurata, Hitoshi Hatakeyama, Azumi Yamashita, Shusa ...
    49 巻 (2014) 1 号 p. 7-15
    公開日: 2014/03/25
    ジャーナル フリー
    In 2008, several episodes of mortality were recorded in cultured populations of juvenile greater amberjack reared in the southwest region of Japan. Diseased fish had asymmetrical abdominal distention and pale gills. The head kidney, trunk kidney, and spleen of every fish that was examined was enlarged and discolored. The results of all microbiological and molecular biological assays of tissues taken from diseased fish were negative for major known pathogens. Histopathologically, the disease was characterized by proliferative interstitial nephritis and proliferative splenitis associated with minute, round structures within the cytoplasm of proliferating mono-nucleated cells. Transmission trial using the enlarged trunk kidney from a naturally infected fish successfully reproduced the disease. The results indicate that this disease is caused by an infectious microorganism, and the most likely etiological agent is the minute, round structures which are probably a hitherto unknown eukaryotic microorganism.
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  • Nuria Castro, Sabela Balboa, Soledad Núñez, Alicia E. To ...
    49 巻 (2014) 1 号 p. 16-22
    公開日: 2014/03/25
    ジャーナル フリー
    Tenacibaculum soleae is a recently described pathogen that has been reported as the causative agent of considerable losses in sole cultures in Spain. This report documents the first case of T. soleae as an etiological agent of tenacibaculosis in farmed sea bass Dicentrarchus labrax. Its identification was performed employing phenotypical, serological and molecular methods. Although the sea bass isolates were homogeneous from a biochemical, chemotaxonomic and molecular point of view, they belonged to a serological group different from the type strain CECT7292, which can be of great importance in the development of future vaccines and other methods of disease prevention in sea bass industry. Virulence assays with a representative isolate confirmed the pathogenic potential for sea bass. The disease was experimentally reproduced by prolonged bath, while no mortalities could be recorded by intraperitoneal injection. The results presented here show that T. soleae must be taken into account as an important pathogen in the marine aquaculture industry.
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