Susceptibilities of 7 fish cell lines, RTG-2, CHSE-214, FHM, EPC, CSF-1, EK-1 and EO-2 cells, to 5 eel viruses, EV-I(EVE), EV-II, EV-III(EVEX), EV-IV and EV-V were studied.
The viruses were subcultured for 3-5 times on monolayer cell sheets in microplates at 20°C and susceptibilities were determined by the replication titers calculated by TCID
50 analysis.
Among the fish cell lines both EK-1 and EO-2 cells derived from Japanese eel exhibited the highest susceptibility to all the eel viruses except EV-II. RTG-2 and CHSE-214 cells showed next higher susceptibility to them. On the other hand, FHM, EPC and CSF-1 cells showed low susceptibility to all these eel viruses. Titers of EK-1 and EO-2 cells to the eel viruses were observed to be more constant than those of RTG-2 and CHSE-214 cells during subcultures.
Above mentioned results indicate the advantage of using eel cell lines for the study of eel viruses.
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