巻号一覧
51 巻, 3 号
選択された号の論文の9件中1～9を表示しています
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総説
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飯田 貴次, 坂井 貴光, 高野 倫一2016 年 51 巻 3 号 p. 87-91
発行日: 2016年
公開日: 2016/10/07
ジャーナル フリーEdwardsiellosis in fish caused by Edwardiella tarda and E. ictaluri is reviewed. Genus Edwardsiella belongs to the Enterobacteriaceae family, and E. tarda produces both hydrogen sulfide and indole, whereas E. ictaluri does neither. E. tarda was first isolated from diseased eel in Japan, and infects freshwater fish as well as marine fish. E. ictaluri has been detected in only freshwater fish after described as a new species from diseased channel catfish in the United States. The two pathogens survive in phagocytic cells, and hosts affected with edwardsiellosis die from septicemia. There were no reports about E. ictaluri infection in Japan before E. ictaluri was isolated from diseased wild ayu in 2007. Recently, the vaccine against edwardsiellosis of Japanese flounder was officially approved in Japan.抄録全体を表示PDF形式でダウンロード (2118K) -
白樫 正, 小川 和夫2016 年 51 巻 3 号 p. 92-98
発行日: 2016年
公開日: 2016/10/07
ジャーナル フリーFish blood flukes (FBFs) are the most important digenean parasites in marine finfish aquaculture due to their high pathogenicity. Numerous eggs accumulate in gill lamellae and capillary vessels in various organs, interfere the blood flow. This causes fish to suffocate to death or leads to other fatal health problems. In Japan, important culture fish, such as amberjacks, bluefin tuna and tiger puffer are affected by FBFs of different taxa; namely Paradeontacylix spp., Cardicola spp. and Psettarium spp., respectively. FBFs are relatively host specific and utilize a complex two-host lifecycle. To date, the lifecycles have been elucidated for only a handful marine FBF species and all use terebellid polychaetes as the intermediate host. Chemotherapy with the oral treatment of praziquantel is effective and commonly used in fish farms as a sole control measure against FBFs. The development of prevention method is expected with the recent advances in knowledge on the biology of FBFs.抄録全体を表示PDF形式でダウンロード (2766K) -
湯浅 啓2016 年 51 巻 3 号 p. 99-102
発行日: 2016年
公開日: 2016/10/07
ジャーナル フリーKoi herpesvirus (KHV) disease caused by cyprinid herpesvirus 3 (CyHV-3) affects carp Cyprinus carpio and its varieties. The disease is listed by the OIE and is also designated as “Specific diseases” in a Japanese law. In Japan, the disease first occurred in 2003 and has quickly spread to all prefectures by distributing infected fish. This review describes basic information on KHV/KHV disease such as its pathogenicity, diagnostic methods, control measures and status of virus distribution in Japan.抄録全体を表示PDF形式でダウンロード (1263K)
論文
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今城 雅之, 森光 一幸, 助田 将樹, 梅﨑 拓也, 門野 真弥, 合田 暉, 久保 栄作, 大嶋 俊一郎2016 年 51 巻 3 号 p. 103-111
発行日: 2016年
公開日: 2016/10/07
ジャーナル フリー高知県野見湾のC. irritansの分布と動態を，5.8S rRNAのTaqManリアルタイムPCRにより調べた。10月に行った20時間の連続測定では，午前4時に最も高いコピー数が得られた。10月上旬と11月下旬に湾口部の漁場のカンパチ生簀で海産白点病が発生したが，この間，被害のあった生簀近傍では，5.8S rRNA遺伝子の平均コピー数から，4,465～6,590虫体/Lの存在が推定された。また，湾奥部に位置する別の漁場でも同程度のコピー数が11月20日と12月16日に検出され，底泥における 5.8S rRNA遺伝子の検出頻度は同漁場で最も高くなり，シストの分布は潮流の影響を受けていると推測された。18S rRNA-ITS1 領域の分子系統解析から，感染漁場のC. irritansは中国分離株を主とするグループに属しており，カンパチ種苗の輸入に伴う中国南部からの持ち込みが疑われた。抄録全体を表示PDF形式でダウンロード (1430K) -
Yuho Watanabe, Sayoko Nishida, Kosuke Zenke, How Kah Hui, Naoki Itoh, ...2016 年 51 巻 3 号 p. 112-120
発行日: 2016年
公開日: 2016/10/07
ジャーナル フリーWe investigated the development of the macronucleus of Cryptocaryon irritans, and the ingestion and digestion of host cells by the parasite. All developmental stages of the parasite except the theront (trophont, protomont and tomont) were examined with histological staining and/or whole-mount staining. The sections were also subjected to in situ hybridization targeting the 18S rRNA gene of the host fish. The macronucleus developed negligibly, maintaining its four-segmented shape in trophonts, elongated and formed a massive nucleus in tomonts before cell division, and subsequently underwent repeated divisions to generate theronts. Denatured host cells, examined in situ hybridization, were accumulated in trophonts, and disappeared in tomonts by the beginning of cell division. Denatured host cells were also observed in the host tissue surrounding trophonts. They had condensed nuclei. Protomonts filled with denatured host cells presented a 180-bp DNA ladder in gel electrophoresis, suggesting apoptosis of the host cells. These results indicate that DNA synthesis occurs exclusively in the early stage of the tomont and that host cells are fed and accumulated, probably as apoptotic cells, in the trophont and are digested in the early tomont stage.抄録全体を表示PDF形式でダウンロード (1667K)
短報
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Hitoshi Nakayama, Etsuo Uno, Takahiro Ashizawa, Satoshi Miwa2016 年 51 巻 3 号 p. 121-124
発行日: 2016年
公開日: 2016/10/07
ジャーナル フリーPlecoglossus altivelis poxvirus-like virus (PaPV) is suspected as the causative agent for atypical cellular gill disease in ayu P. altivelis. The virus was detected by PCR from wild juvenile ayu and Japanese sardine Sardinops melanostictus caught in coastal areas, but not detected from the larval ayu caught in freshwater. No histopathological changes were observed in the gills of juvenile ayu positive for PaPV. When the positive juvenile population was reared in freshwater, the detection rate of the virus gradually decreased and reached below the detection limit 40 days after the stocking.抄録全体を表示PDF形式でダウンロード (761K) -
Chong Yen Thing, Julian Ransangan, Kishio Hatai2016 年 51 巻 3 号 p. 125-127
発行日: 2016年
公開日: 2016/10/07
ジャーナル フリーCaecognathia coralliophila is known as a pathogenic ectoparasite infecting tiger grouper Epinephelus fuscoguttatus in a hatchery in Sabah, Malaysia. The effects of copper sulfate, formalin, trichlorfon, and hydrogen peroxide on the survival of C. coralliophila larvae were tested in vitro. The larvae were exposed to different concentrations of each chemical for 10, 20, 30, 60 min, or 24 h. Trichlorfon was found to be the most effective, killing the parasites within 24 h at 0.2 ppm. Consequently, the toxicity of trichlorfon to tiger grouper was tested. Fish were exposed to trichlorfon at 0.2 ppm for 24 h or 3.2 ppm for 60 min. No fish died in the experiment. Thus, our data suggest that trichlorfon is effective for treating C. coralliophila infection in E. fuscoguttatus.抄録全体を表示PDF形式でダウンロード (517K) -
Sang Phil Shin, Tomoyo Nishimura, Kazuo Ogawa, Sho Shirakashi2016 年 51 巻 3 号 p. 128-131
発行日: 2016年
公開日: 2016/10/07
ジャーナル フリーWe determined the minimum ultraviolet (UV) irradiation dose to influent water to prevent Kudoa yasunagai infection, using microscopy and qPCR. The infection prevalence in Seriola lalandi reared in untreated water reached 45% while that in fish reared in UV-treated water at 5 mJ/cm2 remained below 10%. Additionally, 5 mJ/cm2 UV irradiation significantly reduced spore formation in the brain. No infection was detected when the water was treated with UV at the doses 15 and 30 mJ/cm2. These results indicate that K. yasunagai actinospores are relatively vulnerable to UV irradiation and the minimum effective dose lies between 5 and 15 mJ/cm2.抄録全体を表示PDF形式でダウンロード (570K)
情報
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農林水産省消費・安全局畜水産安全管理課水産安全室原稿種別: 情報
2016 年 51 巻 3 号 p. 132-133
発行日: 2016/09/15
公開日: 2018/12/14
ジャーナル 認証あり平成28年1月27日，輸入防疫及び国内防疫の対象疾病及びその対象水産動物の拡大等の省令改正を行い，我が国の水産防疫体制を強化しました。輸入防疫では，畜水産安全管理課が輸出国政府機関とあらかじめ協議を行い，輸出国における衛生管理体制を確認し，輸入条件及び証明書様式を取り決めます。輸入検査を行う動物検疫所では，新たな輸入防疫対象疾病の検査体制を整えるとともに，輸出国政府機関が発行する証明書及び現物検査で輸入条件を満たしていることを確認し，対象疾病の侵入防止を図ります。国内防疫では，輸入許可を受けた養殖用水産動物について，関係者が連携して6か月間の着地検査を実施し，輸入後の健康状態や疾病発生状況等を把握することとしています。また，都道府県等の検査機関において，新たに指定した特定疾病の検査体制を整えるとともに，サーベイランスの実施により，国内の疾病発生状況を的確に把握します。さらに，養殖場における適切な衛生管理の実施を指導し，国内における特定疾病のまん延防止を図ります。
抄録全体を表示PDF形式でダウンロード (670K)
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