Two new species of the genus Kudoa (Myxosporea: Multivalvulida) were described; Kudoa iwatai sp. n. from the lateral musculature of Pagrus major and Oplegnathus punctatus and K. shiomitsui sp. n. from the pericardial cavity and ventricle of Takifugu rubripes. Both were discovered from fishes cultured at farms on the coast of Kyushu, Japan. Spores of both species were differentiated from other members of the genus Kudoa on the basis of a through recording of their shape, structure and dimentions by means of the light microscope and scanning electron microscope.