魚病研究
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12 巻 , 3 号
選択された号の論文の10件中1~10を表示しています
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  • 城 泰彦, 室賀 清邦
    12 巻 (1977 - 1978) 3 号 p. 151-155
    公開日: 2009/10/26
    ジャーナル フリー
    The present authors have been studing on vibriosis (Vibrio anguillarum infection) in ayu (Plecoglossus altivelis) from the viewpoint of control.
    Prior to the main experiment, several experimental challenge methods were examined in oredr to establish a reproducible artificial challenge system of vibriosis.
    This paper deals with the validity of the artificial challenge system by intramuscular inoculation and a practical application of this system to testing the efficacy of oral-administered drugs for ayu.
    The results are summarized as follows.
    1) The minimal lethal dose of a strain of Vibrio anguillarum used in this study proved to be 8.75 cells or 1×10 mg-8 in wet weight per 100 g of body weight of ayu.
    2) A clear correlation was observed between inoculation dose and mean time to death in the challenge system tested.
    3) A satisfactory result was obtained in the experiment of drug efficacy, and the intramuscular inoculation was found applicable to tests for drug efficacy.
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  • 柏木 哲, 杉本 昇, 大田 外之, 楠田 理一
    12 巻 (1977 - 1978) 3 号 p. 157-162
    公開日: 2009/10/26
    ジャーナル フリー
     養殖ハマチをもちいて人為的に連鎖球菌症感染魚をつくりNFS-Naの治療効果を検討し,以下の結果を得た。1)NFS-Naは50mg/kg/day以上の濃度で,3日間以上連続投与することによって治療効果が認められた。2)対照薬のAB-PCは50mg/kg/dayで5日間連続投与を行っても治療効果が認められなかった。CPは50mg/kg/dayおよび25mg/kg/dayの5日間連続投与で多少の治療効果が認められた。
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  • 宮崎 照雄, 城 泰彦, 窪田 三朗, 江草 周三
    12 巻 (1977 - 1978) 3 号 p. 163-170
    公開日: 2009/10/26
    ジャーナル フリー
     1.徳島県下の養殖場で発生したニホンウナギのビブリオ病を病理組織学的に観察した。2.本病の主要病巣は,皮膚から体側筋組織を巻き込んだ感染病巣であった。その病変は漿液性――出血性――組織壊死性炎で特徴づけられた。3.全身感染症の症例では,脾臓,肝臓,腎臓,腸管などに転移病巣が頻発していた。これら転移病巣における変化は,血行障害を伴う組織壊死であった。
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  • 増村 和彦, 若林 久嗣
    12 巻 (1977 - 1978) 3 号 p. 171-177
    公開日: 2009/10/26
    ジャーナル フリー
     1.人工生産マダイ,クロダイ稚魚に発生する,口吻部の白色ないし黄白色のびらんを主症状とする疾病について調査した。2.発病魚は,マダイ,クロダイとも全長15~60mmの範囲であり,特に20~40mmのものが多い。この範囲内では,大型の個体ほど口吻患部は発赤を伴う。3.患部には濃密な滑走細菌の集落が認められる。菌を分離して,性状,薬剤感受性,病原性について調べた結果,この菌は海水要求性があり,菌体を魚体に塗布することにより人工感染が成立し,自然発病魚と同一症状を呈した。4.この菌は,寒天,セルロース,キチンのいずれも分解しないことからFlexibacter sp.と判断された。
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  • 粟倉 輝彦, 木村 喬久
    12 巻 (1977 - 1978) 3 号 p. 179-184
    公開日: 2009/10/26
    ジャーナル フリー
    A milky condition in smoked coho salmon was observed at a food processing factory in Hokkaido, Japan. The incidence of milky condition was 7.8-11.7 percent of the fish after completion of the smoking process. This condition was due to an infection of the raw salmon, imported from Canada with a myxosporidian parasite identified as Henneguya salmonicola by its characteristic spores.
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  • 中島 健次, 江草 周三
    12 巻 (1977 - 1978) 3 号 p. 185-189
    公開日: 2009/10/26
    ジャーナル フリー
    The first stage larvae of Philometroides carassii(ISHII, 1931) NAKAJIMA et EGUSA, 1977 which were released from the gravid females had very delicate bodies with adhesive sharp pointed tails. They extended and constracted actively in live but were always bent into a siclke-shape when died. They survived in water for seven days at 15°C, five days at 25°C, four days at 2-5°C and several hours at 37°C.
    Drying, freezing, heating and irradiating of ultraviolet rays were very effective to kill them. The larvae were killed instantly when water was evapolated or freezed. Heated larvae died within 18-20 minutes at 40°C, 8-9 minutes at 45°C and a minute at 50°C, and larvae were killed, within 6-8 minutes by irradiation of 2, 000μW/cm2, 14-16 minutes by 1, 000μW/cm2, 43-45 minutes by 400μW/cm2. Larvicidal effect of several disinfectants and anthelmintics were examined in vitro. One ppm of Acrinol, Benzetonium chloride and Sodium dichloroisocyanurate and 10 ppm of Bithionol and Dithiazanine iodide was demonstrated to kill larvae within three days.
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  • 中島 健次, 江草 周三
    12 巻 (1977 - 1978) 3 号 p. 191-197
    公開日: 2009/10/26
    ジャーナル フリー
     1. 鮒糸状虫の中間宿主として3種のケンミジンコ類が確認された。2. ケンミジンコ類は積極的に鮒糸状虫の仔虫を捕食し,摂取された仔虫は5時間以内に胃壁を貫通して血体腔内に寄生する。3. 血体腔内の仔虫は侵入後2日目から急速に発育し,5日後に大部分の仔虫が発育を完了するが,発育に伴なって起こると推察される脱皮は確認できなかった。4. 多数の仔虫に感染したケンミジンコ類はこれに耐過できず,多くは短時間で死滅した。
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  • 金井 欣也, 若林 久嗣, 江草 周三
    12 巻 (1977 - 1978) 3 号 p. 199-204
    公開日: 2009/10/26
    ジャーナル フリー
    Quantitative and qualitative investigations of intestinal microflora were made on healthy and diseased eels collected from culturing ponds in Yoshida, Shizuoka Prefecture. The whole intestine of each eel was homogenized in sterile 0.85% NaCl solution. Tenfold dilutions were prepared and 0.1 ml of which was inoculated on the surface of nutrient agar (Difco). Plate cultures were incubated for five or seven days at 25°C and colonies were counted. Every colony within a given area of the plate of suitable dilutions was subcultured for identification.
    The numbers of viable bacteria per gram of intestinal tract with contents in healthy eels were ranged from 10 to 109 through the year. The percentage of Aeromonas spp. (mostly A. hydrophila) was relatively higher in winter, while that of Enterobacteriacea was higher from spring to fall. Vibrio sp. appeared only in October. Streptococcus sp. was always present 20% to 80% in the intestine of fed eels. Viable counts of intestinal bacteria of diseased eels were 10 to 106 higher than those of the healthy ones (Fig. 1 and Table 4). The percentage of A. hydrophila tended to be higher (Fig. 2), especially in the case of red disease. Moreover, virulent strains from eels suffered from red disease were greater in number than those from healthy ones or eels infected with Edwardsiella tarda (Table 5).
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  • 粟倉 輝彦, 河村 博
    12 巻 (1977 - 1978) 3 号 p. 205-207
    公開日: 2009/10/26
    ジャーナル フリー
    The larvae of Digramma alternans were found on the Japanese dace, Tribolodon hakonensis inhabited Hidaka-horobetsu River in Hokkaido. This is a first finding of D. alternans in fish inhabited rivers in Japan. It was assumed that the first intermediate host (copepods) and the final host (birds) inhabit the swamps which are located around the mouth of the river and lead to it.
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  • 安永 統男, 山元 宣征
    12 巻 (1977 - 1978) 3 号 p. 209-214
    公開日: 2009/10/26
    ジャーナル フリー
    The present study was carried out to reveal the characteristics of organism responsible for socalled vibriosis prevailing in cold-water season among cultured red sea bream, Pagrus major.
    Nine strains obtained from diseased red sea bream in some culture farms in Nagasaki Prefecture in the mid-winter of 1977 were submitted to the morphological, biochemical and physiological characterization. All the strains were gram-negative, nonsporning short rods with one polar flagellum.Cytochrome oxidase and catalase reactions were all positive. Glucose was fermented with no gas production by these strains. In consideration of these characteristics, the present isolates were tentatively ascribed to Vibrio, although many differences were observed among these strains in the other characteristics. On the basis of the decarboxylation of lysine, arginine and ornithine, it was possible to divide these stains into three groups. In general, the temperature of 20-27°C, the NaCl concentration of 2-3%and the pH of 6-8 were optimal for the growth in peptone water.
    Future taxonomic or genetic studies will be necessary to define the present isolates accurately.
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