魚病研究
Online ISSN : 1881-7335
Print ISSN : 0388-788X
ISSN-L : 0388-788X
45 巻, 3 号
選択された号の論文の9件中1~9を表示しています
論文
  • A. M. Shadrin, D. S. Pavlov, M. V. Kholodova
    2010 年 45 巻 3 号 p. 103-108
    発行日: 2010年
    公開日: 2010/09/14
    ジャーナル フリー
    Results of long-term investigation of infection prevalence of fish eggs and larvae with the endoparasite Ichthyodinium sp. (Dinoflagellata) in Nha Trang Bay (South China Sea, Vietnam) are presented. The parasite was identified on the base of morphological characteristics and phylogenetic analysis with 18S rRNA sequences. After the first record in 1993 the infection rate steadily increased till 2004, remainded high in 2004-2007, decreased in 2008-2009 and again boosted in 2010. Seasonal and annual dynamics of the infection rate was described. The parasite showed different degrees of prevalence in different taxonomic groups of fishes. The infection rates were different among closely related species. This is the first report on long-term dynamics of Ichthyodinium infection in Southeast Asia.
  • Pham Minh Duc, Shinpei Wada, Osamu Kurata, Kishio Hatai
    2010 年 45 巻 3 号 p. 109-114
    発行日: 2010年
    公開日: 2010/09/14
    ジャーナル フリー
    Acremonium sp. NJM 0672, isolated from diseased mantis shrimp Oratosquilla oratoria, was susceptible in vitro to three kinds of antifungal agents: voriconazole, amphotericin B and terbinafine hydrochloride. Voriconazole was selected to treat kuruma prawn Penaeus japonicus, which had been intramuscularly injected with 0.1 mL of 5.0 × 104 conidia/mL of Acremonium sp. Voriconazole was administered orally at doses of 6 and 2 mg/kg body weight per day for 7 consecutive days, or intramuscularly injected at doses of 4 and 2 mg/kg body weight per day for 3 consecutive days. Both treatments were begun at 6 h after injection of the conidial suspension. The gross features, mortality and histopathological findings demonstrated that voriconazole was an efficient antifungal agent against Acremonium sp.
  • Akira Kumagai, Akatsuki Nawata, Yuichi Taniai
    2010 年 45 巻 3 号 p. 115-120
    発行日: 2010年
    公開日: 2010/09/14
    ジャーナル フリー
    Occurrences of bacterial cold-water disease (BCWD) caused by Flavobacterium psychrophilum were monitored among released and wild ayu Plecoglossus altivelis in Hirose River in 2006 and 2007 in order to clarify the infection source. When hatchery-reared juvenile ayu were examined immediately prior to release, F. psychrophilum was not isolated. However, when fish from the same lots were reared until maturation, the bacterium was isolated from ripe fish, indicating that the released juvenile ayu were carriers. In epizootiological investigations in Hirose River, outbreaks of BCWD occurred from June to August in both years, but the source of BCWD seemed to differ: released ayu in 2006 and wild ayu in 2007. Additionally, the disease occurred in released ayu by the pathogen carried by themselves in 2007. The disease subsided in August when water temperature exceeded 20°C. Thereafter, the prevalence of F. psychrophilum in ripe fish in the river increased to above 90% between October and November in both years.
  • Osamu Kurata, Nilubol Kitancharoen, Atushi Fujiwara, Chihaya Nakayasu, ...
    2010 年 45 巻 3 号 p. 121-129
    発行日: 2010年
    公開日: 2010/09/14
    ジャーナル フリー
    The role of leukocytes from the kidney of Japanese flounder Paralichthys olivaceus in the encapsulation response was investigated using an in vitro model. The aggregation response of leukocytes to the target fish pathogen Ichthyophonus hoferi was observed over 12 h. The cellular aggregates mainly comprised granulocytes, which were characterized by intracellular peroxidase expression. Moreover, studies of isolated granulocytes demonstrated their adhesion to I. hoferi and formation of an initial layer in the encapsulation response. We observed that mRNA expression levels of three CC-chemokines (CCL3, CCL4 and CC-CLM) and one CXC-chemokine (IL-8) increased during the encapsulation process. Among these chemokines, CC-CLM and IL-8 were actively produced by granulocytes upon stimulation by I. hoferi. Flounder granulocytes therefore appeared to play an important role in the process by releasing specific chemokines upon pathogen recognition, thereby inducing subsequent cellular recruitment leading to encapsulation.
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