In our previous report, retarding effects for tumor growth, of living tubercle bacilli wereobserved with inoculated tumors in mice. The present experiment deals with the relationshipbetween BCG inoculation and tumor induction in mice with INH or urethane-4 NQO injections.
A dd 0 female mouse was intraperitonealy injected with 2mg/0.2ml of INH saline solutionperiodically for 15 weeks (total dosis 60mg). Nine months after, pulmonary adenoma wasinduced in 15 out of 24 mice (62.5%). This tumor induction was markedly depressed by intravenous injection with dried living BCG (four injections with 0.5mg BCG vaccine per mouse intotal). Tumor induction rate was 11.7% in this group.
In another experiment, dd 0 female mice were subcutaneously injected with each 10mgurethane once a week for 11 weeks, and afterwards were given, for further 10 weeks, foursubcutaneous injections with each 0.5mg of 4 NQO. Thirty weeks after, tumor induction wasobserved in 17 out of 20 mice (85.0%). One intravenous injection with 0.2mg BCG vaccinehad not any inhibiting effect for tumor induction. No tumor induction was observed in nontreated control and in BCG inoculated mice.
There was no pathological difference between induced tumors With INH and urethane-4NQO injections. All induced tumors were pulmonary adenoma and no malignant change wasobserved.
The authors were very much interested in the fact that cholesterine pneumonia was foundin many cases of INH-adenoma, the reason of which is not clear at present.

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NAD-ase of human type tubercle bacilli was studied in detail. The enzyme activity wasso weak that it had not been detected under the conditions reported in the preceding paper (Kekkaku 39, 40, 1964), but was detected when the reaction time was prolonged extremely.In comparison with the enzyme activities of guinea-pig tissues or of Mycob. avium, that oftubercle bacilli was very low as shown in Fig. 1-3.
NAD-ase activity of H37Rv was always very weak whenever it was cultured on Sautonsynthetic medium, Dubos tween-albumin medium or Ogawa egg medium.
The enzyme activity of freshly isolated strains was also very weak.
Such low activity was not due to the permeability-barrier.
Isoniazid-analogue formation hypothesis for the action mechanism of isoniazid was discussedonce again as having some difficulties.

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Comparative studies were carried out to investigate the proliferations of avian type tuberclebacilli and nonphotochromogens in developing eggs.
A volume of 0.2ml of mycobacterial cell suspension of 2 mg/ml was inoculated into yolk sacof 5 day old developing egg. After an appropriate time of incubation proliferations of mycobacteria were estimated by counting microscopically and by culturing. The experimentsshowed avian type tubercle bacilli grew more rapidly than nonphotochromogens. These twogroups of mycobacteria influenced differently on the hatching of the developing eggs afterintra-yolk sac inoculation, i. e., the eggs inoculated with nonphotochromogens hatched completely, while a quarter to a half of the eggs inoculated with avian type tubercle bacilli did not hatch.
Several strains of “unclassified mycobacteria” not yet classified were examined by this experimental method and their growth characteristics in yolk of developing eggs were discussed.

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In order to analyse the effect of chemotherapy on tuberculous cavity, histological findings ofcavities were observed in relation to the findings of tubercle bacilli and to clinical findings.From these observations, the healing process of cavity wall and the way of appearance of “opennegative syndrome” were discussed. The observed materials were 199 cavities of 191 cases, whicn showed a cavity on X-ray film before chemotherapy, and were treated without sucha combination as collapse therapy. The cavities were divided into 4 types (non-sclerotic walled, sclerotic walled, cavitation of tuberculoma (Kd), and cavities disappeared during chemotherapy), according to the preoperative X-ray findings.
1. Histological findings of the cavity wall by the length of chemotherapy: In the casesshowing non-sclerotic walled or sclerotic walled cavities on preoperative X-ray films, caseousmass on cavity wall showed the tendency to be excreted during chemotherapy, but the amountof tubercle bacilli decreased till the 3rd month without decrement after that. The thickness ofgranulation tissue increased till the 3rd month but was constant after that. The degree ofhyperemia and new formation of capillary blood vessels of granulation tissue became markedlyrelated to the length of chemotherapy. The thickness of the fibrotic tissue of cavity walldid not change so markedly after the 4th month, and the infiltration or tubercles of pericavitary lung tissue were observed till the 3rd month. As for Kd-type cavities, the relationbetween the amount of tubercle bacilli and the length of chemotherapy was not distinct.In the cases, cavities of which disappeared during chemotherapy, different histologicalfindings were found according to the degree of improvement of cavities on X-ray films.
2. Relation between histological findings and findings of cavity wall on X-ray films: Asfor sclerotic walled cavities, the thickness of the cavity wall on X-ray film was in proportionto the amount of caseous mass on cavity wall on histological slices, and, to some extent, tothe thickness of the fibrotic tissue of the cavity wall, but showed no correlation with the thickness of granulation tissue. Some cavities had no caseous mass on the wall when the shadows, of cavity walls were 2 mm in thickness, while no caseous mass was seen in high percentage when thier thickness was 1 mm. The amount of tubercle bacilli on the cavity wall had correlation to the duration of negative spumut before operation in sclerotic walled or non- sclerotic walled cavities, and most of the cases which showed negative sputum for a period over 7 months before operation had no or only a few bacilli on the wall of cavities. In some of Ka-type cavities, however, many bacilli were found on the surface of caseous mass, while clinical data showed a long period of negative sputum. Such cases were often found in inspis- sated cavities.

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Comparative study was made on the diagnostic value of 70 mm radiophotogram by Odelcacamera and Canon mirror camera. The results of basic experiments were presented in thisreport, and the results of clinical experiments will be mentioned in the second report.
Pictures were taken by both cameras using tube voltage from 65 to 80 kV at 5 kV interval.Correlation between the density of picture and the tube voltage was shown in Fig. 5, and thedensity of picture by Odelca camera was slightly higher than that by Canon camera on thesame tube voltage.
The density of pictures by both cameras was measured along the middle horizontal line, andthe results were presented in Fig. 6. The density was highest in the central part, and becamelower in the peripheral part. The decrease of density in the peripheral part was slightly lessin the case of Canon camera than that of Odelca camera.
The resolving power of cameras was measured by three different methods. In the first experiment, the maximum number of lines clearly distinguished in 1 cm was measured by usingthe test chart made of thin leaves of lead and cupper, which was set in the middle part ofthe fluorescent screen. This chart was used as the standard test chart in U. S. A. As shownin Fig. 7, number of lines clearly distinguished in 1 cm was more in the case of Odelca camerathan that of Canon camera. In the second experiment, test chart made of cupper wire set in the'different width was used, and the minimum width in which two lines were distinguished wasread. The minimum width of two lines in which they were clearly recognized was 0.3 mm inboth cameras, and two lines with width 0.25 mm or less were not distinguished by Canoncamera. In the case of Odelca camera, two lines with width 0.25 mm were not clearly butrecognized as two lines. In the third experiment, test chart enacted in the Japanese IndustrialStandard shown in Fig. 3 was used. Charts were set on nine different spots shown in Fig. 2, and the number of dials in which two lines were clearly distinguished was read. The results were shown in Fig. 8. The resolving power was highest in the central part, and becamelower in the peripheral part. The resolving power in the central part was nearly equal inboth cameras, but the decrease of resolving power in the peripheral part was more marked inthe case of Canon camera than that of Odelca camera. In the case of Odelca camera, betterresults were obtained by using Fuji film than by using Gevaert film in all parts of the fluorescent screen, and in the case of Canon camera, better results were obtained by using Gevaertfilm in the central part, and no difference of resolving power by the film was observed in themiddle and the peripheral parts.
Metal net of hexagonal shape shown in Fig. 4 was set on the focus side of the screen, andmeasurement of distorsion was made along the diagonal. The ratio of length of hexagon inthe longitudinal and horizontal direction in the center to those in the different spots wascalculated, and the results were presented in Fig. 9. The distorsion of picture by Canoncamera in the peripheral part was more marked than that by Odelca camera.
The results mentioned above show that the optical ability of Odelca 70 mm camera was slightly but clearly superior to that of Canon 70 mm mirror camera.

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2 ml of bacterial suspension of tubercle bacilli in Dubos liquid medium were mixed with thesame amount of melted agar in a Widat test tube.
Superposition of drug solution upon the agar plug inhibited the bacilli to grow in the uppermost layer of the agar culture.
The bacilli grew in the depth and formed a colonial disc below the inhibitory zone.
The same was observed with superposition of serum from patient under chemotherapy. Thedepth of the inhibitory zone can indicate the antimycobacterial activity of drug or serum.
These observations were reported by us previously in detail.
The purpose of this paper is to report the subsequent results obtained from our antimyco- bacterial activity test of patients' sera under usual chemotherapy. The maximum dilution ofserum for minimum inhibition was measured. The serum was tested 6hrs after administrationof drugs. The results are as follows.
In the following description “SM 1.0g+INH 0.1 g” or the like will mean a serum froma patient who was given SM 1.0 g+INH 0.1 g or the like. 8× (+) will mean that the serumis active up to 8 fold dilution. 2× (-): the serum is inactive with its 2 fold dilution.
1. Most of “SM 1.0 g+INH 0.1 g” or “PAS 10/3 g+INHG 0.3g” showed 8× (+). None ofthem was 2× (-).“INH 0.1 g +PAS 10/3 g” or “INH 0.1 g+CS 0.25 g” were mostly 2× (+) × (+). Some ofthem were 2× (-).“PAS 10/3 g+INH 0.2 g” is more active than “PAS 10/3 0.1 g”.
Most of “PAS 10/3 g +TH 0.3 g” were more active than “PAS 10/3 g+INH 0.2 g”.“CS 0.25 g alone” were 2× (-) with few exceptions. Nevertheless it is worthy of note that“CS 0.25 g;+INH 0.1 g” is comparable to “PAS 10/3 g+INH 0.1g”.“PZA 1.0 g alone” were also 2× (-).“PZA 1.0 g+INH 0.2 g” or PZA 1.0 g+INHG 0.5 gshowed activity.
2. In the same patient, “PAS+PINHG” was stronger than. “PAS+TH”. But, in a few cases, it was reverse.
In every case, “PAS+INHG” was stronger than “PZA+INHG”.
In a few cases, every combination examined gave a strong activity.On the contrary, also in a few cases, every combination gave only a weak activity.3. Antimycobacterial activity of CS and TH.With 1.5 γ/ml TH superposition, in a week, the agar plug brought up bacterial colonies in the top layer of it. With 3.1 γ/ml TH superposition, it brought up colonial disc in a deeplayer, about 0.7 cm from the surface. With 6. 2 γ/ml, 12.5γ/ml the distance was 1.3 cm and1.5 cm respectively.
In 10-14 days, another colonial disc appeared in the previous inhibitory zone.
The same was observed in a case of CS 50 γ/ml or “PASH+TH” superposition. These phenomena suggested the differences between TH, CS and SM, KM, VM, PAS, INH.
4. The serum antimycobacterial activity demonstrated in this way was considered to beattributed to the drugs administered. The test was considered to be usefull in evaluatingantimycobacterial activity of serum from patient under chemotherapy.

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