巻号一覧
55 巻, 11 号
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豊原 希一1980 年 55 巻 11 号 p. 473-479
発行日: 1980/11/15
公開日: 2011/05/24
ジャーナル フリーThe antituberculous activity of 27753R.P. (R.P.), a semisynthetic derivative of Griselimycine, was studied experimentally both in vitro and in vivo. Results were as follows:
1. Antituberculous activity in vitro of R.P. was different by media. M.I.C. was 0.5μg/ml both in Kirchiner liquid media and in Dubos liquid media, but the growth of tubercle bacilli was almost inhibited by 50μg/ml in 1% Ogawa's media. The results showed that the antituberculous activity was decreased in Ogawa's media.
2. 27753R.P. did not show cross resistance to other known antituberculous drugs.
3. 27753R.P. was effective both for the experimental tuberculosis in mice and guinea pigs.
4. 27753R.P. was effective for M. kansasii in vitro, but not effective for M. intracellulare.抄録全体を表示PDF形式でダウンロード (597K) -
束村 道雄, 水野 松司1980 年 55 巻 11 号 p. 481-484
発行日: 1980/11/15
公開日: 2011/05/24
ジャーナル フリーStrains of Mycobacterium aviurn-Mycobacterium intracellulare complex could be divided into two subgroups, one showing no detectable spots and another showing two radioactive spots at the Rf values 0.10-0. 14 and 0.32-0.36, by thin-layer chromatography (TLC) of a fraction extracted with acetone after incubation with 35S-sulfate.
The strains tested were cultivated on Ogawa egg medium, harvested after incubation at 37 °C for 10 days, and washed twice with a 0.9% NaCl solution. One hundred mg (wet weight) of cells were weighed and added aseptically to the following reaction mixture: 3.0ml of M/15 phosphate buffer solution (pH 7.1) containing 0.1% sodium acetate and 5μCi/ml, 35S-sulfate. The reaction mixture was incubated at 37°C for 24 hours, and the cells were centrifuged. The cells were washed twice with 2.0ml of distilled water and extracted twice with 10% trichloroacetic acid (TCA) solution. The cells were then extracted twice with 2.0ml of petroleum ether, and the petroleumether extracts were discarded. The cells were extracted twice with 2.0ml of acetone, each time for 5 minutes. The acetone extracts were combined and dried by allowing to stand at 37°C. The dried material was dissolved in 0.1ml of acetone, and its 0.02ml sample was placed onto a Silica Gel Hlayer (20 by 20 cm; 0. 25 mm thick) with a micropipette. The sample was subjected to TLC using a solvent: n-propanol-n-butanol-water-ammonia (57: 20: 20: 3, in volume) (ascending system). The chromatogram was then scanned for the radioactivity by an automatic TLC scanner (Nihon-Musen Co., Tokyo) (Scanning speed, 300 mm/hour; chart speed, 150 mm/hour; range, 1, 000 counts perminute (cpm); slit, 30×6 mm; time constant, 30 seconds). The 35S-sulfate was a product of the Radio chemical Centre, Amersham, Buckinghamshire, England (Sodium sulfate (35S) injection, 0. 63 mCi/ml).
The results are shown in Table 1 and Fig. 1. The radioactive spot that has appeared at the Rfvalue 0.95 is originally petroleum ether-soluble. The TLC of the concentrate of the petroleumether extracts showed only one spot at this Rf value and the peak was as high as more than 1, 000 cpm.
The strains were divided into two subgroups by the absence or presence of some acetone-soluble radioactive compounds. The first subgroup did not show any radioactive spot and the strains showingserotypes 1, 2, 7 and 14 belonged to this group. The second subgroup consisted of the strains which showed the serotypes 9, 16, 18, and 20. Strains which had a M. scrofulaceum serotype 43 (Gause) also entered this group. The former showed no detectable, radioactive spots and the latter showed two radioactive spots at the Rf values 0.10-0.14 and 0.32-0.36.抄録全体を表示PDF形式でダウンロード (492K) -
特に薬剤耐性との関係について桜井 宏, 渡辺 善正, 山中 正彰, 森口 敏勝, 藤井 久弥, 上ノ山 利雄1980 年 55 巻 11 号 p. 485-491
発行日: 1980/11/15
公開日: 2011/05/24
ジャーナル フリーThe status on admission and therapeutic effects of chemotherapy were observed in 167 pulmonary tuberculosis patients with positive culture and history of antituberculosis chemotherapy, who were hospitalized during January 1976 through December 1979 and stayed for more than 4 months at our hospital. The results were summarized as follows:
1) Status on admission; 74% were male, their age distribution was 46% in the age-group 410 years and 30% in 61 years or more. On radiographic assessment, 76% of the patients had cavitation with sclerotic wall. Sensitivity revealed that 58% showed drug-resistant bacilli to more than 1 drug and 20% to 3 drugs or more of SM, INH, EB, KM and RFP.
2) All patients received chemotherapy and 140 of them treated with RFP for 4 months or more. Fifteen patients died in the hospital and 138 patients were discharged, 52% within 6 months and 90% within 12 months. At the time of discharge 15 (10%) patients had positive-culture and 89 (64 patients had cavitation.
3) Bacteriologic progress during chemotherapy with RFP; 105 patients were treated with RFP. INH and either one of SM, KM or EB. Among them, 70 patients were sensitive to all drugs before treatment, and 68 (97%) had favorable response; and out of 15 patients with starins resistant to SM (KM or EB), only one showed an unfavorable response, but out of 20 patients with INH-resistant strains, negative conversion was obtained in 13 (65%) patients.
4) Bacteriologic relapse; Bacteriologic relapse of 114 patients showing a favorable bacteriologic response during treatment with RFP as observed from 6 to 36 months after negative conversion, and only 2 patients relapsed within 12 months.
5) Emergency of drug-resistance; out of 12 patients sensitive to RFP and remained positiveculture during treatment with RFP, RFP-resistant strains appeared in 10 patients within 6 months and the rate of emergence of resistance to combined drugs was also very high.
It is concluded that regimens containing RFP was highly effective for retreatment cases with drug-sensitive strains, despite the severity of the disease; and as the significance of resistance to combined drugs, resistance to INH was especially important.抄録全体を表示PDF形式でダウンロード (875K) -
Kazuhisa SAITO1980 年 55 巻 11 号 p. 493-512
発行日: 1980/11/15
公開日: 2011/05/24
ジャーナル フリーPDF形式でダウンロード (2787K) -
1980 年 55 巻 11 号 p. 513
発行日: 1980/11/15
公開日: 2011/05/24
ジャーナル フリーPDF形式でダウンロード (88K)
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