Oily substances with ester bonds and fatty acids in n-hexane solution were determined by an attenuated total reflection (ATR) /FT-IR. In this experiment, the solution was dropped on an internal reflection element (IRE) and the residue on IRE was analyzed after n-hexane was evaporated. When the amount of the residue on IRE was from 0.5 to 5μg, triolein and oleic acid could be determined from the absorbance data of peaks close to 1745 cm
-1and 1710 cm
-1, respectively. Though various fatty acids exist in the soil from the human body, they could be determined using the calibration curve for oleic acid. When the amount of interfering substances was small, the influence on the determination of triolein and oleic acid could be neglected.
ATR/FT-IR analysis was compared with gravimetric analysis on the determination of oily substances extracted from an undershirt used for 6 months. As a result, when oily substances with ester bonds were determined using the calibration curve for a mixture of 65% triolein and 35% palmitic acid n-hexadecyl ester, the ATR/FT-IR analysis was nearly equal to the gravimetric analysis. This method enables a speedy and simple determination of oily substances with ester bonds and fatty acids from the human body on fabrics.
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