In order to clarify the heat stress effects, under ambient air temperature and humidity, on thermoregulation, psychological responses, autonomic nervous and salivary amylase activity of the human body, 6 young female subjects wearing only shorts and a brassiere were exposed to 9 conditions consisting of air temperature of 28℃, 31℃ and 34℃ and 30%, 60% and 90% relative humidity for 60 minutes. The results of the analysis of variance are summarized as follows: ①Mean skin temperature, sweat rate, skin moisture were significantly increased with an increase in air temperature and humidity, but rectal temperature showed no significant differences. ②Thermal, humid and uncomfortable sensations changed to the uncomfortable side with increasing temperature and humidity. ③Heart rate, LF (Low Frequency)/HF (High Frequency) and salivary amylase activity were significantly increased with an increase in air temperature and humidity, while HF showed only tendency to be decreased. ④High correlation coefficients between skin moisture and uncomfortable sensations (r=0.888), humid sensations (r=0.982), salivary amylase activity (r=0958) and LF/HF (r=0.910), suggested a dominant indicators of the heat stress. Salivary amylase activity and LF/HF were also suggested as an effective indicator of heat stress.

For proposing a state of dyeing of hematein in the wool fiber, the identification of metal species forming a complex with hematein was investigated by means of ICP-MS analysis and the analysis of K/S-wavelength (λ) curves of the hematein-dyed samples. The contents of three metal species (copper, zinc, and ferrous) in the wool fiber, formic acid treated wool fiber, silk fiber were determined by ICP-MS analysis. These results indicated that the amount of zinc included in wool fiber was contained considerably more than that of the silk fiber, and in addition that the amount of zinc is drastically reduced by formic acid extraction treatment. Next, it was found that the K/S-λ curves of the hematein-dyed silk fabric which is mordanted by copper sulfate and zinc sulfate solution were similar to that of the hematein-dyed wool fabric. However, if both the mordanted silk fabrics and the hematein-dyed wool fabric was adjusted to pH 4.0, the K/S-peak originating from the coordination between hematein moiety and metal ion in the zinc-mordanted silk fabric and the hematein-dyed wool fabric has disappeared, nevertheless the peak of the copper-mordanted silk fabric was still remained. On the basis of these findings, we proposed the schematic diagram for the coordination between hematein moiety and zinc ion in the cuticle-cuticle CMC at 4.0 and 7.0 of the internal pH.