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56 巻, 11 号
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3. マウスを対象としたMycobacterium intracellulare吸入感染の試み李 英徹, 久世 文幸, 前川 暢夫, 鈴木 康弘1981 年 56 巻 11 号 p. 513-519
発行日: 1981/11/15
公開日: 2011/05/24
ジャーナル フリーAn experimental attempt to induce Mycobacterium intracellulare infection in white conventional mice was made by means of an airborne infection apparatus. Mycobacterium intracellulare, TMC 1469 strain (provided by the U. S.-japan Cooperative Medical Science Program-NIAID in 1975), was grown in Dubos Tween albumin liquid medium for ten days and stored at 4°C until each airborne challenge. The exposure apparatus used was an Airborne Infection Apparatus, Model A42, TRI-R Instruments, INC (New York, U. S. A.). At each airborne challenge, the bacillary culture was diluted five fold in distilled water (ca. 1.4×107 v. u./ml) to be placed in the nebulizer, and the animals were exposed for 40 minutes to a cloud of fine droplet nuclei containing the bacilli. The mice were divided in three groups and each group underwent airborne challenge respectively for single, five and fourteen times. After the airborne infection, mice were sacrificed and quantitative culture of lungs and histopathological examinations of main organs were performed at different intervals.
In the single airborne challenge group, the viable units of bacilli in 10mg of lung was 3.0×102immediately after the challenge, and decreased gradually to roughly one-hundredth of the count at fourteen weeks after the challenge. In the groups of five and fourteen times airborne challenges, the number of viable units were between 1.4×10-3.1×103 and 9.3×102-5.2×103 respectively during the experimental periods. Thus no significant increase in the number of viable units in lungs was recognized in the three groups.
The histological changes were exclusively seen in the lungs of mice which underwent five and fourteen times airborne challenges. Mononuclear cell aggregates at various sites were most frequently seen. One mouse, sacrificed at five weeks after five times challenge, showed a granulomatous lesion. Neither proliferative nor diffuse exudative lesions were revealed, and the histological changes were thought to be weaker and less significant compared with those of infection by M. tuberculosis.
In view of these results, further endeavours would be necessary to induce experimental Mycobacterium intracellulare disease.抄録全体を表示PDF形式でダウンロード (3534K) -
第3編薬剤耐性獲得について山鳥 英世1981 年 56 巻 11 号 p. 521-524
発行日: 1981/11/15
公開日: 2011/05/24
ジャーナル フリーIn the previous reports, unfavourable conditions for the growth of tubercle bacilli were prepared by replacing air with various other gases using Kirchner's medium containing various drugs, and the bacteriostatic and bactericidal effects of anti-tuberculous agents were investigated.
In this report, the development of drug resistance of M. tuberculosis which was not killed by the bactericidal tests was investigated.
The development of drug resistance was not observed, regardless of the duration of drug contact with bacilli and of exposure to several kinds of gases.抄録全体を表示PDF形式でダウンロード (484K) -
持続排菌例の家族よりの患者発生に関する検討亀田 和彦, 矢野 周子, 久池井 暢, 堀井 富士子, 大塚 順子, 中原 歌子1981 年 56 巻 11 号 p. 525-530
発行日: 1981/11/15
公開日: 2011/05/24
ジャーナル フリーAccording to our previous four years follow-up study of favourable response to family contacts of newly registered cases showing good course treatment, 90% of new patients were found during the time of the first two years after the detection of index cases. It was therefore concluded that the family contacts examination should be carried out in the first two years after the discovery of index cases, and further examination would be less necessary.
The survey in this report was made to know whether different patterns in the outbreak of new patients are found or not in the family contacts of cases showing positive bacilli for longer than two years after the registration (so called chronics) and how the family contacts examination should be done for such chronics.
Study subjects were 340 family members of 106 chronics registered as of 1930 at 12 health centers in Osaka Prefecture. At this time 180 (52.4%) out of 340 were examined and one new patient was found. In addition, one another case was found by symptomatic visit to clinic. Adding these two cases to 43 who had had previous history of treatment, altogether 45 cases were found in family con tacts, and 34 (75.6%) out of 45 were discovered during the first four years after the discovery of index cases, and 30 out of 34 were infants or school children. Four out of 19 infants and school children who were born after the index cases became chronics had been diagnosed as primary infection or re quiring treatment before they reach five years of age.
The high incidence rate of new patients was similarly observed in the initial two years both in family contacts of cases showing favourable response to treatment and of chronics. But in the latter cases, the rate was two times higher than the former and did not decrease so markedly until the fourth year.
It can be concluded that, in contrast to cases showing favourable response to treatment, the con tacts examination of chronics should thoroughly be carried out for the first three to four years after the discovery of index cases, especially for infants and school children. The same careful consider ation should be given to infants who are born after the index cases became chronics.抄録全体を表示PDF形式でダウンロード (927K) -
佐藤 明正, 宮本 覚, 庄村 東洋, 中井 準, 吉栖 正之, 束村 道雄1981 年 56 巻 11 号 p. 531-534
発行日: 1981/11/15
公開日: 2011/05/24
ジャーナル フリーMycobacterium avium-M. intracellulare complex opportunely causes human pulmonary disease. This species generally gives a negative reaction to the niacin test.
The present paper describes the isolation of a strain of niacin-positive M. avium-M. intracellulare complex from lung lesions of a patient. The characteristics of the isolate are shown in Table 1.
The niacin test was performed by the qualitative and the quantitative methods which were slightly modified from the original description by Konno.
In the qualitative method, the positive reaction to the niacin test, i.e., pink coloring, was observed in the isolated strain.
In the quantitative method, the organism was incubated on the synthetic liquid Sauton medium for three weeks. After incubation, the liquid medium was kept at 100°C for one hour, and then filtrated through the Toyo filter paper. The bacilli were dried and weighed. The volume of the filtrate was measured, and a 2 ml sample of the filtrate was mixed with 1 ml of 10 per cent cyanogen bromide solution and 1 ml of ammonium-buffer solution. The reaction color was measured spectrophotometrically at 405 mμ. These tests were performed simultaneously to the isolated strain, M. tuberculosis H37Rv and M. intracellulare ATCC 15984. The results are shown in Figure 1. Whereas the niacin production of M. intracellulare ATCC 15984 was less than 1.0μg per 1 mg of the dried bacilli, the isolated strain produced about 3 μg and H37Rv produced about 8 μg. This quantitative method showed that the isolated strain produced apparently more niacin than M. intracellulare.抄録全体を表示PDF形式でダウンロード (2287K) -
島尾 忠男1981 年 56 巻 11 号 p. 535-559
発行日: 1981/11/15
公開日: 2011/05/24
ジャーナル フリーPDF形式でダウンロード (3833K)
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