In order to estimate the activity in cultured ascidian,
Halocynthia roretzi, we examined the effects of chemical (MgCl
2) stress and followed physical (high temperature) stress on ascidians which had been reared in running water, stagnant water with aeration and without aeration conditions, through the continuous monitoring of squirting.
Mg treatment reduced the squirting of ascidians reared in stagnant condition compared with control ascidian, which had been reared in running water, although they did not show significant difference under running water treatment.
High temperature treatment, followed Mg treatment, increased the squirting of control ascidian. For the others, it transitorily emphasized the squirtings, but decreased and stopped them in several hours. Of the ascidians reared in stagnant condition, the ascidian, which was reared without aeration suffered a severe effect rather than ascidian reared with aeration.
These results suggested that the monitoring of squirting in the chemical and physical stress conditions could evaluate the activity of ascidian.
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