This study was undertaken to examine the occurrence of
Vibrio parahaemolyticus in the cultured Japanese horse mackerel
Trachurus japonicus.
V. parahaemolyticus was detected in 20 to 80% of fish specimens collected in the warmer season with mean densities of 3.6×10
2 to 5.4×10
3 MPN/100 g. In water samples, low densities of
V. parahaemolyticus (36 MPN/100
ml) were detected at water temperatures of 22.8-27.5°C. PCR amplification was performed for four strains of
V. parahaemolyticus isolated from the fish, and all isolates examined were an avirulent type (
toxR gene-positive and
tdh- and
trh-negative).
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