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Volume 46, Issue 6
Displaying 1-8 of 8 articles from this issue
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Toshizo SAKURAI, Hiromi ISHIWA1972 Volume 46 Issue 6 Pages 273-280
Published: 1972
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSTreating a lysogenic strain of Lactobacillus casei IAM 1043 by nitrosoguanidine, 4 strains of heat inducible mutants of the temperate phage were isolated.
In the cells lysogenized with these mutant temperate phages, shifting the culture from 30°C to temperature over 36°C for a certain period of time resulted in conspicuous lysis at 150 minutes following the heat treatment. When the culture was shifted from 30°C to 40°C, maintenance of the cells at 40°C for 10 minutes was sufficient to induce the phage. The higher the temperature of the heat treatment is, the shorter the period re-quired for the heat induction. The inducibility also depends on the age of the cells and the pH of the culture medium.
An attempt was made to induce the cells lysogenized with the mutant phages in a 100 litre tank.View full abstractDownload PDF (1160K) -
Shohei FUJINAWA, Kozo OGAWA, Hiroshi KIMURA, Hiromi NAKATANI1972 Volume 46 Issue 6 Pages 281-287
Published: 1972
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSA method of industrial application of Trametes sanguinea enzyme (A-12-C) to the drained cherry processing was established. According to the treatment with A-12-C, the canding time could be reduced from 14 days to 6 days, and the product had no shrinkage in shape, excellent mouth-feel in texture. Furthermore, the product was obtained in high yield.
The main enzyme activities of A-12-C are cellulase, polygalacturonase, acid protease and β-1, 3-glucanase, but the effective enzyme activities for the drained cherry processing were found to be those of cellulase and polygalacturonase.View full abstractDownload PDF (521K) -
Keisaku KOMEDA, Teh-chou CHANG, Itsuo ICHIMOTO, Hiroo UEDA1972 Volume 46 Issue 6 Pages 289-294
Published: 1972
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSThe Mannich reaction of 4-methyl-2-pentanone in both acidic and basic media has been extensively studied. In the acidic medium, 4-methyl-2-pentanone reacts with morpholine and formaldehyde to give 1-N-morpholino-5-methyl-3-hexanone (4) and 1-N-morpholino-2-isopropyl-3-butanone (5), respectively, in good yield (81%, (4)/(5)=69/31). On the other hand, the reaction in basic medium afforded the same products ((4)/(5)=8/92) in 43% yields. The time-course variation of this reaction was also examined and the mechanism for the reaction is suggested. The structure of the products was elucidated by chemical evidences and spectroscopic data.View full abstractDownload PDF (412K) -
Eiji NIWA, Tsuneo KONAGAYA, Masato MIYAKE1972 Volume 46 Issue 6 Pages 295-301
Published: 1972
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSA simplified food texturometer was manufactured for trial as a improvement type upon the existing plunger test apparatus for the measuring of elastic properties (Ashi) of Kamaboko. The feature of this instrument is that the mechanical stress to be applied to the sample during the running in of the plunger can be recorded automatically on graph paper.
Experiments were made not only on various types of Kamaboko, those containing starch, those produced from tuna, and those on the market, but on fish sausage, indicating satisfactorily good accuracies of this method.View full abstractDownload PDF (471K) -
Shin-ichi FUJITA, Yasuji FUJITA1972 Volume 46 Issue 6 Pages 303-307
Published: 1972
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSThe essential oil of Mentha Pulegium Linn. which was kept for about 2.8 years has been examined, especially for the high-boiling components, together with the auto-oxidation products and the following compounds were determined.
Neutral compounds: α-pinene, β-pinene, limonene, 3-octanone, p-cymene, 3-octyl-
acetate, 3-octanol, 1-octen-3-ol, 3-methylcyclohexanone* [3], menthone [2], isomenthone, isopulegone [6], pulegone [1], piperitone, cis- and trans-pulegone-oxide* [4], [5], piperi-tenone, dehydroxymenthofuran-oxide* [9], menthofuran-oxide* [10], caryophyllene, β-humulene, and n-paraffins (C18_??_C52).
Acidic compounds: lauric acid, myristic acid, palmitic acid, β-methyl-adipic acid* [7], β-methyl-δ-isobutyryl-valeric acid* [8], phenol, o-cresol, p-cresol, salicylaldehyde, and eugenol.
Among these compounds, the star-marked are the auto-oxidation products.View full abstractDownload PDF (359K) -
Saburo TAKANO, Takao SUZUKI, Yoshikazu SAHASHI1972 Volume 46 Issue 6 Pages 309-312
Published: 1972
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSInhibitory activities by amino acids or their derivatives upon a rice blast fungus, Piricularia oryzae Cavara P2 (C-1) 6019, were studied by observing the colonial growth of the fungus on a medium. The growth of the fungus was inhibited by L-methionine, L-serine, L-arginine at the 1% significance level, while L-aspartic acid stimulated the growth thereof.
Amino acid derivatives such as N-benzoyl-L-methionine, N-benzoyl-L-glutamic acid, N, N'-dibenzoyl-L-lysine, N-benzoyl-L-isoleucine, N-benzoyl-L-valine, N, N'-diphenacetyl-L-cystine, N-phenoxyacetyl-L-leucine, N-2, 4-dichrolophenoxyacetyl-L-leucine were in-hibitory upon the appearance of colonies at a significance level of 1%.
Judging from the above results, some of amino acid derivatives may inhibit the growth of the rice blast fungi.View full abstractDownload PDF (221K) -
Hideko NAKAMURA, Kenji WATANABE, Junya MIZUTANI1972 Volume 46 Issue 6 Pages 313-317
Published: 1972
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSAs taste substances in Warabi (Pteridium aquilinum var. latiusculum Und.) and Yamadori-zenmai (Osmundastrum cinnamomeum var. fokiense Tagawa), free sugars were examined. The sugars were extracted by conventional means, tentatively identified by thin-layer chromatography, and were converted to O-trifluoroacetyl-sugars, which were then quantitatively analyzed by gas chromatography on Silicone DC QF-1.
The linear temperature-programmed procedure was used to separate an authentic mixture of rhamnose, arabinose, xylose, fructose, galactose, mannose, glucose, sucrose, maltose, lactose and inositol into each component. Linearity of area ratio (sugar/inositol) versus molar ratio (sugar/inositol) was confirmed with arabinose, fructose, glucose and sucrose, respectively.
Glucose and fructose were detected, and glucose was the major component (70_??_80%) of free sugars of Warabi and Yamadori-zenmai.View full abstractDownload PDF (301K) -
Seisuke ITO, Yasuhiko FUJINO1972 Volume 46 Issue 6 Pages 319-322
Published: 1972
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSIt has been known to be troublesome to isolate individual glyceroglycolipid from plant tissues probably because of coexistence of pigments, neutral lipids, phospholipids, isoprenoids and so forth. This time, we could isolate relatively easily the three glycero-glycolipids, monogalactosyl diglyceride, digalactosyl diglyceride and sulfoquinovosyl diglyceride from alfalfa leaves largely by silicic acid column chromatography with chloro-form-acetone.
The present paper describes the isolation technique, together with the analytical data of the respective glyceroglycolipid thus prepared.View full abstractDownload PDF (809K)
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