Limonin 17-β-D-glucopyranoside, total limonoid glucosides and limonin in the juice, pericarp and endocarp of hassaku (
C. hassaku Hort. ex Tanaka) were determined by HPLC and TLC. Hassaku juice contained 314 ppm of total limonoid glucosides, whereas pericarp and endocarp tissues contained 2300 ppm and 3100 ppm, respectively, on dry basis. Limonin 17-β-D-glucopyranoside was the predominant glucoside derivative in the juice (148ppm, 47% of total limonoid glucosides). However, nomilin 17-β-D-glucopyranoside was the predominant glucoside in the pericarp and endocarp tissues. In addition to limonin and nomilin glucosides, the fruit tissues also contained the glucoside derivatives of typical citrus limonoids, such as nomilinic acid, deacetylnomilinic acid and obacunone.
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