All issues
Volume 23, Issue 8
Displaying 1-12 of 12 articles from this issue
- |<
- <
- 1
- >
- >|
-
Kazuo YAMAFUJI, Hirohisa OMURA1950 Volume 23 Issue 8 Pages 321-325
Published: March 01, 1950
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSIn our laboratory it has been established that hydrogen peroxide exerts specific actions upon the formation of virus molecules in living cells. According to our studies hydrogen peroxide is formed always as a result of tissue respiration. We have found that under the suitable conditions hydroxylamine causes a remarkable decrease of catalase activity in living tissues. But in such a case the respiration does not suffer changes in its intensity. It is supposed that accumulation of hydrogen peroxide takes place in the bodies fed with this amine. Indeed hydroxylamine brings about the formation of virus molecules in the cells. As it is possible that hydroxylamine occurs in organisms in the form of oximes, we fed silkworms with acetoxime. Then we could ascertain that by this treatment the virus formation is provoked in worm bodies.View full abstractDownload PDF (479K) -
Part I. The Relationship between the Growth of Lactobacillus and the Composition of Medium. (2)Ichiro YOSHIHARA1950 Volume 23 Issue 8 Pages 326-329
Published: March 01, 1950
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSTitrimetric responses of species of lactic acid bacteria to riboflavin were tested in relation to the microbiological assay. Lactodacillus acidophilus seemed. to be the most suitable bacteria among those tested. (The results are shown in Figure I.)
Fundamental experiménts for the determination of riboflavin with Lactobacillus acidophilus were carried out.
The acid production of Lactobacillus acidophilus is increased in parallell with the amount of riboflavin added, up to apapproximately 0. 15 microgram per 10ml.
The acid production of Lactobacillus acidophilus is not affected by temperature change of 37° ± 1°, but at 37°_??_38° the acid production is apparently increased than at 36°_??_37° (The results are shown in Figure 2.)
When glucose and photolyzed peptone are added simultaneously to the medium reported by SNELL, the growth and acid production of Lactodacillus acidophilus is extremely increased without added riboflavin. However, without the simultaneous addition of glucose and peptone, addition of one or more components can not give rise to such increase, between 0.03 and 0.11 microgram of riboflavin per 10ml.
On standing after autoclaving, the medium may become defective. (The results are shown in Figure 3. 4 and 5 and in Table 6.)
The possibility of microbiological assay of riboflavin with Loctobacillus acidophilus was shown, but on application to various materials, it is necessary to notice that the growth and acid production by Lactobacillus acidophilus can be affected not only by the amount of ribofavin, but also by other substances in the solution tested.View full abstractDownload PDF (299K) -
Megumu INAOKA, Haruko WADA1950 Volume 23 Issue 8 Pages 330-335
Published: March 01, 1950
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSOn the basis of many experiments in our laboratory we have established a hypothesis which concerns the terminal process of tissue respiration. The hypothesis demands that in all cases of oxidations by oxygen in the cells hydrogen peroxide is formed as final product. In order to, ascertain this hypothesis we have made some experiments. It has been found that the respiratory capacity of yeast cells is extremely resistant to the poisonous action of hydroxylamine. But the activity of cytochroms is remarkably hindered by this amine. Urethane causes a little decrease in yeast respiration. Under special conditions we have been able to confirm that the formation of hydrogen peroxide takes place even in the case of oxidation on charcoals.View full abstractDownload PDF (593K) -
Prt I. The Mechanism of Formation of Shoyu Oil (1)Takashi KUBO1950 Volume 23 Issue 8 Pages 335-342
Published: March 01, 1950
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSI studied the biochemical mechanism of formation of Shoyu oil, which has the peculiar properties as compared with the fat in raw materials, and obtained the following results.
(1) The fat in raw materials was largely decomposed before the fermentation and it contained about 60% free fatty acids after hydrolysis.
(2) In the course of fermentation, ethyl esters of fatty acids were synthesized not rela-ting to its lapse before fermentation.
(3) The solid matter which appears in the winter, was contained only in the Shoyu oil which is obtained in the period of fermentation.
(4) Unsaponifiable matter and Reichert-Meiszl value increased as fermentation proceeded.View full abstractDownload PDF (610K) -
Katsue AKATSU1950 Volume 23 Issue 8 Pages 343-348
Published: March 01, 1950
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESS1) Some strains of Aspergillus japonicas produce an antibiotic substance against the pellicle forming yeast, which is harmful to “Shoyu”
2) These strains produce the antibiotic substance in modified Czapeck-solution (4% lactose, instead of sucrose) at 25°_??_30° after 14 day culture, and this culture liquor inhibits Zygosaccharomyces sulsus (the chief of pellicle forming yeasts of “Shoyu”) at a dilution of 1:500_??_1500.
3) Have not yet isolated the antibiotic substance purely, but it was confirmed that this substance had at least one carbo_??_yl radical, and obtained the crude Na-salt (water soluble), Ba-salt (water-insoluble), and Ag-salt(water-insoluble) as powder.
The method of extraction and pur;fication to that stage are described in this paper.
4) The antibiotic activity of the crude Na-salt (powder) is shown at a dilution of 1:100, 000_??_1, 000, 000 against yeasts, but the activity is not against moulds (1:500_??_2000), and is scarce against bacteria.View full abstractDownload PDF (435K) -
Part IV. Retting of Manila Hemp by Molds and Wood Destroying FungiT. ASAI, S. NAKAMURA1950 Volume 23 Issue 8 Pages 349-353
Published: March 01, 1950
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSWe isolated a useful mold from the contaminants of wood destroying fungus culture on the saw dust. This, mold belongs to a variety of Aspergillus niger and shows the most excellent result for the retting of manila hemp than any other molds and wood destroying fungi which were tested in our experiment.
For the preparation of inoculum we adopted the solid culture method on saw dust con-taining some nutrients, i.e., the powder of stalk and leaf of sweet potato, alcohol stillage and husk of tea leaf. It was confirmed that these nutrients were utilised to promote the growth and enzymic action also in the course of main retting. The condition of retting are as follows viz., the quantity of liquid medium added is about four times to the raw fibers, the temperature lies between 25°_??_30°C, the pH range is about 5_??_7, and the time required for retting is about 8_??_10 days. The optimum degree of retting is indicated from the decreasing ratio of lignin content, that is, 30_??_45% of its decreasement is suitable for the spinning. The yield of fibers is about 90% to raw materials.View full abstractDownload PDF (413K) -
Part V. Retting of Ramie Fiber by MoldsT. ASAI, T. NAKANISHI1950 Volume 23 Issue 8 Pages 354-356
Published: March 01, 1950
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSA useful mold for retting of ramie fiber was isolated from the cotton stalk. This mdld belongs to a variety of Aspergillus varians. Several conditions of retting were investigated and it was confirmed that the optimal pH was abont 3.5 and the optimal temperature lied between 35 and 37°. The retting is perfectly finished in 3 days under the optimal conditions. Addition of nutrients is not necessary, because the raw ramie fibers contain sufficient nutrients for the growth and enzymic action of the mold. An extracted enzyme solution is also useful for retting.View full abstractDownload PDF (243K) -
Part II. The Production of Ultraviolet-induced Mutations in Aspergillus sojae (continued)Nobuyoshi IGUCHI1950 Volume 23 Issue 8 Pages 357-360_1
Published: March 01, 1950
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSConidia of a selected strain of Aspergillus sojae (No. 260) were exposed to ultraviolet radiation, and random isolations were made from the colonies resulting from irradiated cells. Approximately two hundred of these were cultivated upon Czapek's agar solution. The majority of the isolates remained stable with normal appearance and similarity to cultural mutants. Eleven types of mutations were recognized. One of these resulted from restricted colony. Another was unable to utilize nitrate nitrogen-but developed normally if supplied with Koji extracts. Other mutations showed a total or partial loss of color without apparent alterations in their physiological properties.
These eleven types are as follow : 1. Normal, 2. Restricted, 3. Extremely, restricted 4. Albino, 5. Olive, 6. Yellow, 7. Brown, 8. Sterile, 9. Floccose, 10. Nitratophobe, and 11. Nitrate non assimilative.View full abstractDownload PDF (871K) -
Part VII. On Further Improvement of Method of Isolation of Crystalline Beef Liver CatalaseMasaharu SHIRAKAWA1950 Volume 23 Issue 8 Pages 361-362
Published: March 01, 1950
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSChanges of the amout of extractive beef liver catalase by autolysis were studied. After 20 hours' autolysis of beef liver at 25°C increases the amount of extractive catalase by about 40 per cent. On this basis, an improved method of isolation of crystalline catalase was described.View full abstractDownload PDF (188K) -
Part II. On the Puriflcation and the Utilization of Alginic Acid prepared from Sargassum Horneri (TURN.) AG. and Sargassum mioracanthum YENDOY. TOMIYASU, T. HARA, M. MIYAKE1950 Volume 23 Issue 8 Pages 362-365
Published: March 01, 1950
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSThe effects of the various purification methods…… the chlorine method, the copper sulphate method, the basic lead acetate method, the hydrochloric acid method and the repeating method which is carried out by repeating the filtration and precipitation processes alternately…… were investigated by us. and it was found that the hydrochloric acid method was the most useful one.
Some experiments on the utilization of alginic acid for the preparation of an artistic paint, an opaque glass and a furniture paint were also described.View full abstractDownload PDF (399K) -
Past III. A New Method for Clarifying Alkaline Extract of Alginic Acid-containing MaterialsY. TOMIYASU, M. MIYAKE1950 Volume 23 Issue 8 Pages 365-367
Published: March 01, 1950
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSIt is well known that the filtration of the alkaline extract of alginic acid-containing materials is very difficult. Several methods for obtaining; clear extract have been proposed thus far, but these are not thought as satisfactory method.
In the present paper a new rapid and simple method, which is able to clarify the extract without using any filtration process, called “Carbontetrachloride method” by us, is described.View full abstractDownload PDF (222K) -
Yoshiyuki INOUYE, Manjiro NODA1950 Volume 23 Issue 8 Pages 368
Published: March 01, 1950
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSDownload PDF (80K)
- |<
- <
- 1
- >
- >|