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Volume 40, Issue 10
Displaying 1-7 of 7 articles from this issue
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Production of γ-Pyrone Compounds by Aspergillus speciesShunji ISHIIE1966 Volume 40 Issue 10 Pages 353-358
Published: 1966
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSThe author made a comparative study on the production of γ-pyrone compounds by 166 type cultures of Aspergillus species in surface or submerged culture in modified Czapek's medium which contained sucrose and peptone as C and N sources, respectively. The results obtained were as follows: (1) Most of γ-pyrone compounds produced by Aspergillus species was kojic acid (KA). (2) Although some variations in the mode of KA production were observed in different strains of the same species, the half numbers of strains belonging to Asp. tamarii and Asp. flavus-oryzae groups were assumed to be good KA producers. (3) A large part of the KA producers showed that the surface culture was more favorable for KA production than the submerged one. (4) Several strains among KA producers were observed to be able to accumulate a new (unidentified) γ-pyrone compound in the latter period of culture.View full abstractDownload PDF (445K) -
Kojic Acid Production by UV-Irradiated Strains of Aspergillus oryzaeShunji ISHIIE1966 Volume 40 Issue 10 Pages 359-363
Published: 1966
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSAs reported previously, Aspergillus strains belonging to Asp. taraarii and Asp. fiavusoryzae groups exhibited the possibilities to produce kojic acid (KA) in both surface and submerged cultures. This paper discusses kojic acid production by UV-irradiated strains of Aspergillus oryzae. The results obtained were as follows: (1) All of the survival strains which possessed the same morphological characters as original strain were assumed to exert the same mode of KA production as original one. (2) The irradiation had “Positive Effect” on both the amounts and rates of KA production, but was ineffective on the whole figure.View full abstractDownload PDF (331K) -
Screening of Alkane-Assimilating Molds and their Oxidative Products from AlkanesKoichi YAMADA, Yasushi TORIGOE1966 Volume 40 Issue 10 Pages 364-370
Published: 1966
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSMany reports have already been published about the studies on the utilization of hydrocarbons by microorganism. However, most of them were concerned with bacteria or yeats and we have only a few reports concerned with molds in this field, so we were much interested in the study on hydrocarbon-utilizing mold. We have isolated 380 strains of molds from 230 kinds of samples (soil, water, vegetables, etc.) by repeating alternately accumulation culture and plate culture techniques in the media containing alkanes and mineral salts. In addition to these 380 strains, 70 strains already stocked in our laboratory as the hydrocarbon-utilizing molds were examined if any organic acids of economic value can be produced by these hydrocarbon utilizers. Stable cultures of these molds were carried out in the medium containing 3.0% of alkanes and mineral salts, and we found that 10 strains out of 450 strains of molds produced organic acids in their broths.
The main acids produced from alkanes were the mono- and diterminal carboxylic acids (e. g., pelargonic and azelaic acids from n-nonane, and capric and sebacic acids from n-decane). We thought it very interesting that we could demonstrate the fact that the mono- and diterminal oxidations take place during the early oxidative steps of alkanes by molds. One of these acid-producing molds numbered S 547 was classified into the genus Botrytis of Imperfect Fungi.View full abstractDownload PDF (1295K) -
On the Stability of Lysosomes from Rabbit-Kidney (1)Yasokichi OGASAWARA1966 Volume 40 Issue 10 Pages 371-376
Published: 1966
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSFreshly prepared lysosomal fraction contains 80 per cent of the total cathepsin in the bound form. The author has examined the behavior of cathepsin bound in lysosomes from rabbit-kidney in order to clarify the stability of the lysosomes. The results of the experiment are as follows: The stability of the lysosomes is dependent upon freezing and thawing, crushing, γ-ray, temperature, and mixed substances. So long as the lysosomal membrane remains intact, digestion of the substances on which the lysosomal enzymes act is confined within the lysosomes. But when the membrane is ruptured, the enzymes leak out and digestion takes place externally, often resulting in digestion of the cell. Therefore, the stabilization of the lysosomes in the fresh foods renders great services to maintain the natural taste in the foods. If the properties of the lysosomes in the living tissues are studied, it may be possible to develop a new good manufacturing and storaging process of the fresh foods.View full abstractDownload PDF (452K) -
Kiyoshi NAKAYAMA, Hiroshi HAGINO1966 Volume 40 Issue 10 Pages 377-380
Published: 1966
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSAn arginine-requiring mutant of a glutamic acic-producing bacterium, Corynebacterium glutamicum (syn. Micrococcus glutamicus) KY 9396, was found to accumulate a large quantity of an amino acid in the culture medium. The amino acid was isolated in crystalline form from the culture medium and was identified as L-citrulline by its melting point, elementary analysis, infrared absorption spectrum and optical rotation.
The cultural conditions for L-citrulline production by this mutant were investigated. In synthetic media, arginine concentration remarkably affected L-citrulline accumlation. The L-citrulline accumulation was maximum at a suboptimum concentration of arginine for the growth of the microorganism, namely 125 micrograms per milliliter. L-Citrulline yield increased as the concentrations of potassium phosphate in the medium decreased until both mono- and dipotassium phosphate became to the level of 0.025%. It was noted that L-glutamic acid accumulation decreased with the increase of L-citrulline accumulation. Replacement of arginine in the medium with yeast extract markedly increased the L-citrulline accumulation. Thus, the amount of L-citrulline accumulation reached to a level of 10.7 milligrams per milliliter with the medium containing 10% of glucose.View full abstractDownload PDF (288K) -
Michihiro SUGANO, Isao CHINEN, Masafuto WADA1966 Volume 40 Issue 10 Pages 381-386
Published: 1966
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSFatty acid compositions of the liver lipids from White Leghorn normal male ano laying female chickens, which can be easily obtainable from local hatcheries and fed on commercial diets, have been determined. 1. The main components of the liver fatty acids were, in order of decreasing percentage composition, stearic, palmitic, oleic and linoleic acids for the males, and oleic, palmitic, stearic and linoleic acids for the laying females. 2. There was no appreciable difference in the fatty acid composition of cholesterol ester fraction, both qualitatively and quantitatively, between the males and the females. This fraction had the highest percentage concentration of linoleic acid and the lowest of palmitic acid compared to other lipid fractions. 3. The triglyceride fraction was characterized by higher percentage composition of oleic and saturated acids. Quantitatively, the laying hen contained oleic, palmitic and linoleic acids approximately 10-fold compared with those of the males. 4. The phospholipid fraction had the highest percentage concentration of arachidonic and stearic acids among lipid fractions separated. There was only small difference between the males and layers in the composition and quantity of fatty acids in this fraction.View full abstractDownload PDF (490K) -
Akira ÔBAYASHI1966 Volume 40 Issue 10 Pages R53-R57
Published: 1966
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSDownload PDF (414K)
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