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Volume 40, Issue 12
Displaying 1-9 of 9 articles from this issue
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A Design of the Apparatus for Temperature Gradient IncubationToshitaka NAKAE, Takeo NAKANISHI1966 Volume 40 Issue 12 Pages 431-436
Published: 1966
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSAn apparatus for incubating the organisms by means of a temperature gradient system under steady state conduction of liquid was devised. This consists of cylindrical temperature gradient bath mounted between two circulating water baths which are regulated separately at constant temperature, upper bath being always warmer than the lower bath. A series of different, constant temperatures of liquid filled in the temperature gradient bath can be established simultaneously. Water is a suitable heat t ansfer liquid for this purpose. Incubation tubes containing solid media are then inserted vertically into the temperature gradient bath. Visible growing zone of the organisms appears at a difinite portion of the agar column corresponding to temperature range for growth. The apparatus is considered to be applicable to rapid estimation of temperature relation with microbiological activities as well as to simultaneous isolation of psychrophiles, mesophiles and thermophiles.View full abstractDownload PDF (1252K) -
The Relationship between the Chemical Structure of the Dyes and their Antibacterial ActivityItaru ISHII, Masako KATAGIRI, Kaiichiro SAKAZUME, Tomomasa MISATO1966 Volume 40 Issue 12 Pages 437-442
Published: 1966
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESS760 photosensitizing dyes were tested for bactericidal activity in vitro against Xanthomonas oryzae, leaf blight bacteria of rice plant. It was found that 205 dyes, which were cyanine- or styryl-type, were effective for inhibiting growth of X. oryzae, four dyes of which at a concentration of 0.l μg/ml completely inhibited the growth of X. oryzae. The relationship between the chemical structure of photosensitizing dyes and their antibacterial activity was discussed. In greenhouse, using rice seedlings inoculated artificially with X. oryzae, effect of photosensitizing dyes was tested for control of leaf blight, and it was found that several dyes were highly effective.View full abstractDownload PDF (391K) -
Thermal Decomposition Products of CelluloseKunio KATO, Toshiko DOIHARA, Fukumi SAKAI1966 Volume 40 Issue 12 Pages 443-448
Published: 1966
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSCellulose powder was decomposed thermally under atmospheric pressure at 350°C. Furfural and acetaldehyde have been isolated from the products as their 2, 4-dinitrophe-nylhydrazones and the following compounds have been identified by paper- and gas chromatography: formic, acetic, propionic, iso- and n-butyric acids, propionaldehyde, butylaldehyde, phenol and o (m)-cresol. The amounts of furfural and acids (formic and acetic) were 1.4% and 1.1% of cellulose, respectively. The water content was about 79% of products and it corresponds to 30% of cellulose dry matter. The weight loss of cellulose when heated at continuously rising temperature in air was remarkable at a temperature range of 250_??_350°C. Both formation of furfural and destruction of cellulose were also extremely high under the conditions applied herein. From the results of quantitative determination of furfural among the decomposition products of cellulose and levoglucosan, it was suggested that cellulose was not always decomposed through levoglucosan.View full abstractDownload PDF (422K) -
Lowering Effect of Phenolic Compounds and o-Diphenoloxidase on the Digestibility of Protein in Pasture PlantsTakao HORIGOME, Makoto KANDATSU1966 Volume 40 Issue 12 Pages 449-455
Published: 1966
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSEleven pasture plants were studied for total phenol, o-diphenol, o-diphenoloxidase, and in vitro digestion of their proteins. Amount of phenolic compounds and o-diphenoloxidase activity varied with plant species, but the occurrence of o-diphenoloxidase was accompanied by that of o-diphenolic compounds. Content of o-diphenolic compounds in fresh plants decreased by drying at 65°C with aeration and the reduction seemed to be partly related to the o-diphenoloxidase activity of the plant. If the acetone powder from fresh plants had been previously incubated at pH 6.3 for 1 hr. without pepsin, the digestibility of the protein with pepsin (pH 1.5) became lower in response to its higher amount of o-diphenol and o-diphenoloxidase activity. The same observation was true of trypsin digestion (pH 8) regardless of whether acetone powder was preincubated or not. The lower digestibility of the protein in dried plant seemed to be closly related to the amount of o-diphenol lost by the drying of plant. These results suggest that the interaction of proteins with o-diphenolic compounds and o-diphenoloxidase in pasture plants lowers the digestibility of the proteins.View full abstractDownload PDF (553K) -
On the Acidic and the Phenolic ComponentsMasayoshi YAMAZAKI1966 Volume 40 Issue 12 Pages 456-460
Published: 1966
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSThe acidic and the phenolic fractions of the volatile components of mulberry leaves were examined by gas chromatography and thin-layer chromatography. (1) In the acidic fraction, besides those found already, two unknown components were found, one of which had large proportion in it. In the phenolic fraction, phenol, eugenol, o-cresol, m (and/or p)-cresol, guaiacol, methylsalicylate were identified qualitatively for the first time. (2) Moreover, from the calibration curves of their major components, approximate quantitative analysis was presented for each of the components of both fractions.View full abstractDownload PDF (305K) -
Part V Flavonoid Pigments from Pollen of Lilium lancifolium ThunbYoshihisa TOGASAWA, Teizo KATSUMATA, Hideo KAWAJIRI, Norimasa ONODERA1966 Volume 40 Issue 12 Pages 461-465
Published: 1966
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSCrystalline flavonoid pigments I and II were isolated from the pollen of Lilium lancifolium Thunb. in the yields of 0.056% and 0.036%, respectively. Melting points, color reactions, RF values on paper chromatogram, UV and IR spectra of the pigments I and II were similar to those of authentic rutin and narcissin, respectively. On hydrolysis with sulfuric acid, glucose and rhamnose were detected from both pigments, while quercetin was isolated from the pigment I and isorhamnetin from the pigment II. Therefore, the pigments I and II were confirmed to be rutin (quercetin-3-rutinoside) and narcissin (isorhamnetin-3-rutinoside), respectively.View full abstractDownload PDF (1082K) -
Quantitative Analysis of ZincTadahiro MASUMURA, Michihiro SUGAHARA, Syujiro ARIYOSHI1966 Volume 40 Issue 12 Pages 466-468
Published: 1966
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSThe application of atomic absorption spectroscopy to the rapid quantitative analysis of Zn in formula feed and its ingredients was investigated. (I) Absorbance at 213.9 mμ was measured for Zinc. (2) Linear relationship between absorbance and concentration was found at 0_??_40 p. p. m. for Zinc. (3) The sample solutions were prepared by dissolving the samples in hydrochloric acid after dry ashing, and were atomized directly into the flame of the atomic absorption device. The content of Zn in the sample solutions was calculated from the calibration curve. (4) Any significant interference from other ions was not observed. Accuracy and reproducibility of this method were satisfactory. The analytical values obtained by this method agreed with those obtained by other analytical method (spectrophotometric method with dithizone). (5 ) It was concluded from this study that the atomic absorption spectroscopy could be successfully applied to the rapid analysis of Zinc in formula feed and its ingredients.View full abstractDownload PDF (232K) -
Quantitative Analysis of Manganese and CopperMichihiro SUGAHARA, Tadahiro MASUMURA, Syujiro ARIYOSHI1966 Volume 40 Issue 12 Pages 469-473
Published: 1966
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSAtomic absorption spectroscopy was applied to the rapid quantitative analysis of Mn and Cu in formula feed and its ingredients for their quality control. (1) Absorbance at 279.8 mμ was measured for Mn, and 324.7 mμ for Cu. (2) The sample solutions were prepared by dissolving the samples in hydrochloric acid after dry ashing. (3) Mn in formula feed and its ingredients was determined by atomizing the sample solution directly into the flame of the atomic absorption device. (4) Since the content of Cu in formula feed and its ingredients was too low to determine Cu by direct atomizing of the sample solution, it was necessary to extract Cu with Na-diethyldithiocarbamate into ethyl acetate before atomizing. If the content of Mn is too low (0.5_??_5mg% in sample), it is determined as same as the procedure of Cu estimation. (5) Satisfactory recoveries for Mn and Cu were obtained from formula feed. (6) Analytical values of Mn and Cu obtained by the atomic absorption spectroscopy well agreed with those by spectrophotometric method. (7) The coefficients of variation for determination of Mn and Cu in formula feed were 1.66% and 5.14%, respectively.View full abstractDownload PDF (393K) -
Ryuhei FUNABIKI1966 Volume 40 Issue 12 Pages R67-R74
Published: 1966
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSDownload PDF (571K)
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