Nippon Nōgeikagaku Kaishi

Online ISSN : 1883-6844
Print ISSN : 0002-1407
ISSN-L : 0002-1407

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Volume 61, Issue 6

Displaying 1-17 of 17 articles from this issue

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Discovery of Hyper Protein-Producing Bacteria and Their Utilization to the Production of Useful Proteins

Shigezo UDAKA

1987Volume 61Issue 6 Pages 669-676
Published: June 15, 1987
Released on J-STAGE: February 18, 2009

DOIhttps://doi.org/10.1271/nogeikagaku1924.61.669

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Filtration Membrane Fouling in Ultrafiltration of Skim Milk

Masanobu KOUTAKE, Yukio UCHIDA, Takayoshi SATO, Kousou SHIMODA, Atsuo ...

1987Volume 61Issue 6 Pages 677-681
Published: June 15, 1987
Released on J-STAGE: February 18, 2009

DOIhttps://doi.org/10.1271/nogeikagaku1924.61.677

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Skim milk was ultrafiltrated to concentration factor (CF) 6 at 50°C, 3.0kg/cm², and 2.4m/sec. Module UFP 10 (Rhone-Poulenc Co.) equipped with a polyacrylnitrile (PAN) membrane or a polysulfone (PS) membrane was used.
Visible gels were observed on both membranes after ultrafiltration (UF) of reconstituted skim milk but not after OF concentration of fresh skim milk. Fluxes of both membranes were almost the same and were about 10l/m²•hr at CF 6.
The fouled membranes were cleaned successively in three steps: (1) water cleaning, (2) hand cleaning with a soft sponge to remove deposits on the membrane surface and (3) alkali detergent cleaning. Fluxes were not restored by steps (1) and (2), but step (3) restored the membrane. The protein contents of the alkali-cleaning solution increased with cleaning time. These results suggested that membrane fouling here is probably due to the milk proteins in the membrane pores.

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Filtration Membrane Fouling in Ultrafiltration of Skim Milk

Masanobu KOUTAKE, Yukio UCHIDA, Takayoshi SATO, Kousou SHIMODA, Toshia ...

1987Volume 61Issue 6 Pages 683-689
Published: June 15, 1987
Released on J-STAGE: February 18, 2009

DOIhttps://doi.org/10.1271/nogeikagaku1924.61.683

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The first report indicated that membrane fouling in OF of skim milk might be due to milk proteins in the membrane pores. The membrane surfaces were observed by a scanning electron microscope (Hitachi S-800) at×50, 000. Most pores were not visible in fouled membranes even after hand cleaning with a soft sponge. In membranes cleaned with an alkali detergent, the pores were visible. Chemical analyses and amino-acid analysis of extracts from unused and fouled membranes showed that the fouling materials were mostly milk proteins, particularly whey proteins. It is concluded that the fouling was not caused by gels or deposits on the membrane surface, but by milk proteins in the membrane pores.

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Cytotoxic Effects on HEp-2 Cells of Trichothecene Mycotoxins and Their Related Macrocyclic Trichothecene Compounds Baccharins B-4 and B-5

Katsuichiro OKAZAKI, Takumi YOSHIZAWA, Susumu KIMURA

1987Volume 61Issue 6 Pages 691-693
Published: June 15, 1987
Released on J-STAGE: February 18, 2009

DOIhttps://doi.org/10.1271/nogeikagaku1924.61.691

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The cytotoxicity of 10 trichothecene compounds was measured by colony-forming inhibition test on HEp-2 cells. The required concentrations for 50% inhibition of colony formation were 0.014 μM for diacetoxyscirpenol, 0.497 μM for neosolaniol, 0.020 μM for T-2 toxin, 1.351 μM for deoxynivalenol, 5.325 μM for 3-acetyldeoxynivalenol, 0.529 μM for nivalenol, 1.166 μM for fusarenon-X, 0.006 μM for satratoxin G, 0.105 μM for baccharin B-4 and 0.041 μM for baccharin B-5. The results indicate that macrocyclic trichothecenes baccharins B-4 and B-5 exhibit moderate cytotoxicity.

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[title in Japanese]

Makoto KITO

1987Volume 61Issue 6 Pages 695-697
Published: June 15, 1987
Released on J-STAGE: February 18, 2009

DOIhttps://doi.org/10.1271/nogeikagaku1924.61.695

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[title in Japanese]

Reiko URADE, Yukako HAYASHI

1987Volume 61Issue 6 Pages 698-700
Published: June 15, 1987
Released on J-STAGE: February 18, 2009

DOIhttps://doi.org/10.1271/nogeikagaku1924.61.698

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[title in Japanese]

Hitoshi TAKAMURA

1987Volume 61Issue 6 Pages 701-704
Published: June 15, 1987
Released on J-STAGE: February 18, 2009

DOIhttps://doi.org/10.1271/nogeikagaku1924.61.701

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[title in Japanese]

Harumi OKUYAMA, Kazuyo YAMADA

1987Volume 61Issue 6 Pages 705-707
Published: June 15, 1987
Released on J-STAGE: February 18, 2009

DOIhttps://doi.org/10.1271/nogeikagaku1924.61.705

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[title in Japanese]

Yasunobu GOTO

1987Volume 61Issue 6 Pages 708-711
Published: June 15, 1987
Released on J-STAGE: February 18, 2009

DOIhttps://doi.org/10.1271/nogeikagaku1924.61.708

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[title in Japanese]

[in Japanese]

1987Volume 61Issue 6 Pages 713-718
Published: June 15, 1987
Released on J-STAGE: February 18, 2009

DOIhttps://doi.org/10.1271/nogeikagaku1924.61.713

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[title in Japanese]

[in Japanese]

1987Volume 61Issue 6 Pages 719-720
Published: June 15, 1987
Released on J-STAGE: February 18, 2009

DOIhttps://doi.org/10.1271/nogeikagaku1924.61.719

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[title in Japanese]

[in Japanese]

1987Volume 61Issue 6 Pages 720-722
Published: June 15, 1987
Released on J-STAGE: February 18, 2009

DOIhttps://doi.org/10.1271/nogeikagaku1924.61.720

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[title in Japanese]

[in Japanese]

1987Volume 61Issue 6 Pages 722-723
Published: June 15, 1987
Released on J-STAGE: February 18, 2009

DOIhttps://doi.org/10.1271/nogeikagaku1924.61.722

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[title in Japanese]

[in Japanese]

1987Volume 61Issue 6 Pages 723
Published: 1987
Released on J-STAGE: February 18, 2009

DOIhttps://doi.org/10.1271/nogeikagaku1924.61.723

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[title in Japanese]

[in Japanese]

1987Volume 61Issue 6 Pages 723a-724
Published: June 15, 1987
Released on J-STAGE: February 18, 2009

DOIhttps://doi.org/10.1271/nogeikagaku1924.61.723a

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[title in Japanese]

[in Japanese]

1987Volume 61Issue 6 Pages 725-726
Published: June 15, 1987
Released on J-STAGE: February 18, 2009

DOIhttps://doi.org/10.1271/nogeikagaku1924.61.725

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[title in Japanese]

[in Japanese]

1987Volume 61Issue 6 Pages 739-740
Published: June 15, 1987
Released on J-STAGE: February 18, 2009

DOIhttps://doi.org/10.1271/nogeikagaku1924.61.739

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