Studies on
l-sorbose production by submerged growth of
gluconoacetobacter roseus nov. sp. in a small rotary drum fermenter revealed the following:
(1)
d-Sorbitol, in concentration up to 30 %, was oxidized perfectly in 48 hours.
(2) Quantities of sorbitol medium should be 25 to 30 percent to the whole quantity of the fermenter; the less sorbitol medium was used, the better was the result.
(3) Quantities of inoculum could be lowered to 3% of the main nutrient solution.
(4) Qualities of sorbitol gave main influences upon fermentation.
(5) Concentrat on of nitrogenous nutrient (extract of fish-meaty) could be lowered to 0.2%, but when concentrated sorbitol medium was used, the concentration of 0.4_??_0.6% was desirable.
(6) Conditions of air flow, air pressure, rate of rotation according to W
ELLS et al. were employed also in these studies almost succesfully.
(7) Remedies for, eventual stoppage of electric current were studied. Several experiments in a large rotary fermenter revealed the same results to those in a small fermenter.
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