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Volume 74, Issue 11
Displaying 1-5 of 5 articles from this issue
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Shunya TAKAHASHI2000Volume 74Issue 11 Pages 1231-1236
Published: November 01, 2000
Released on J-STAGE: November 21, 2008
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Kaoru TAKEGAWA2000Volume 74Issue 11 Pages 1237-1246
Published: November 01, 2000
Released on J-STAGE: November 21, 2008
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Yuji NAGATA2000Volume 74Issue 11 Pages 1247-1257
Published: November 01, 2000
Released on J-STAGE: November 21, 2008
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Hiroyuki SUMI, Tomohiro SASAKI, Chieko YATAGAI, Yasutaka KOZAKI2000Volume 74Issue 11 Pages 1259-1264
Published: November 01, 2000
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSUsing trypsin-fibrin plate method, strong activity of the fibrinolysis accelerating substance (FAS) was proved in water extract of Japanese fermented soybean “natto”. In 7 commercial natto preparations, high concentrations of 74, 100±54, 500 units (equivalent to μg surfactin)/100g wet weight (Mean±SD) were detected. FAS strongly accelerated not only the acitivities of direct fibrin degradating enzymes such as plasmin, trypsin, and earthworm protease, but also that of the plasminogen activator (urokinase) and pro-urokinase activator (nattokinase). It was easily separated as dark yellow powder from natto extract by two-step procedures of acid-precipitation (pH 2.0) and ethanol treatment (soluble fraction). The final yield and purity from 1kg natto were about 817, 000 units (dry weight 4.6g) and 177.6 units/mg, respectively.View full abstractDownload PDF (1132K) -
[in Japanese]2000Volume 74Issue 11 Pages 1275-1276
Published: November 01, 2000
Released on J-STAGE: November 21, 2008
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