Optimum conditions for controlling
Staphylococcus aureus were examined in fermented sausage inoculated with starter cultures and
S. aureus. Inoculation of starter lactic acid bacteria
Lactobacillus plantarum-4 (ST) and
Pediococcus cerevisiae-1 (10
7 cells/g sausage emulsion) effectively inhibited the growth of
S. aureus to less than 10
4 cells/g of fermented sausage emulsion, but the inhibition of
S. aureus (by more than 10
5/g) was not sufficient by inoculation of 10
8 cells/g. To supplement the inhibitory activity, glucoseoxidase was added to the fermented sausage emulsion together with the starter culture. Glucoseoxidase at 4.4 unit/g did not affect the growth of the starter culture but strongly inhibited the growth of
S.aureus by more than 99% after 20 hr incubation at 37°C.
It seemed that these effects arose from hydrogen peroxide generated by glucoseoxidase. The use of a large amounts of glucoseoxidase was unfavorable for color and water-holding capacity of the sausage emulsion. By addition of 8.8 unit/g, the a-value of the sausage emulsion color was reduced to 58% of the control value after 20 hr incubation at 37°C. The optimum fermentation conditions for preventing
Staphylococcal growth and maintaining sausage quality were the addition of 10
7 cells of starter cultures and 2.2 unit of glucoseoxidase/g sausage emulsion.
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