The administration of a diet containing 250 ppm of PCB (Aroclor 1248) to carps caused increased TBA values and decreased amounts of glutathione, vitamin E and substances reactive to 1, 1-diphenyl-2-picrylhydrazyl in the hepatopancreas.
In addition, the treatment of carps with the same diet caused a significant elevation in the activities of both glutathione peroxidase and glutathione reductase in the hepatopancreas, whereas no change was observed in γ-glutamyltranspeptidase activity.
Lipid peroxidation occurred in the 9000×
g supernatant or microsomes of the hepatopancreas in the presence of the NADPH-generating system and Aroclor 1248 at concentrations of 10
-6M and 10
-7M. On the other hand, Aroclor 1248 inhibited the peroxidation at 10
-3M and 10
-4M.
In vitro lipid peroxidation in the presence of Aroclor 1248 required the NADPH-generating system.
Aroclor 1248 inhibited NADPH-cyt.
c reductase and glucose-6-phosphatase
in vitro at concentrations above 10
-5M in the hepatopancreas microsomes.
Aroclor 1248 administration increased the amount of 1-anilinonaphthalene-8-sulfonate (ANS) bound to the carp hepatopancreas microsomes. The
in vitro addition of Aroclor 1248 enhanced the binding of ANS to the hepatopancreas microsomes, and this was more potent at higher concentrations of Aroclor 1248 than at lower concentrations.
The mechanism of lipid peroxide formation in the hepatopancreas of carp ingesting Aroclor 1248 was discussed.
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