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Volume 25, Issue 7
Displaying 1-16 of 16 articles from this issue
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Fujio EGAMI, Mituo EBATA, Ryo SATO1951 Volume 25 Issue 7 Pages 347-349
Published: 1951
Released on J-STAGE: November 21, 2008
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T. NAGASAWA, K. KAWAKAMI1951 Volume 25 Issue 7 Pages 349-351
Published: 1951
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSThe fungistatic power of dehydracetoic acid, was compared with p-hydroxybenzoic acid butyl ester, for the purpose of food preservation.
In this experiment following fungi were used: Monilia nigra, Penicillium glaucum, Pichia, Trichoderma lignorum, and Rhizopus.
Both solid and liquid media are used as culture medium that was made out of Kampyo-extract (dried fruit of Lagenaria vulgaris). The tables were the results of 45 days' observation. They show that dehydracetoic acid has the fungistatic power similar to or higher than p-hydroxybenzoic acid butyl ester.
But it was recognized that the dehydracetoic acid was superior to the p-hydroxybenzoic acid butyl ester on such point as larger solubility in water, tasteless, odourless and smaller toxicity etc.View full abstractDownload PDF (248K) -
Part 1. The Cultureal Environments affected to the Amylase ProductionT. ASAI, S. HOSHI, S. MIYASAKA, M. IZUMIDA1951 Volume 25 Issue 7 Pages 352-357
Published: 1951
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSAmong the selected strains of Aspergillus oryzae group, the strain No. 0-9-5 (which of Tokyo) gave excellent anfylase activity for the submerged production.. By using this strain, several resea-rches concerning the cultural environments affecting the amylase production were carried out. It was shown that the optimal initiall pH for incubation lay between 5 and 6 the activity markedly fell down under 4.0 and amylase action was completely inhibited or destroyed under 3.0. The incubation at 30°C was more favorable than at 25°C. When starch and peptone were taken as C and N sources, the optimal concentrations of both of these substrates were about 2 to 3%, NaNO3 and NH4NO3 were suitable inorganic N sources, because these substances played buffer actior during the incubation, viz., they prevented the acidification due to the acid production. As natura; organic N sources, rice bran, wheat bran, dry yeast and stillage showed the excellent results.
Amylase production, provided that the cultural conditions were normal, reached to maximun after 3 days' incubation which coincided with the maximum utilization of N by fungus mycelliun and then the activity fell down according to the autolysis and pH rising of the culture medium.View full abstractDownload PDF (534K) -
Part 3. Lipase Activity of P. chrysogenum Q176 on Soybean OilShuntaro YASUDA, Hiroe HORI, Akio HIBIYA1951 Volume 25 Issue 7 Pages 357-361
Published: 1951
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSWe obtained the following results from the experiments on lipase activity of P. chrysogenum Q176.
1. Lipase inside the organism is powerful at 24 hours and that outside it is of a low activity.
2. Lipase in the culture media showed its activity during 48 to 72 hours of fermentation.
3. Enzymatic chemical properties were studied: the optimal pH was relatively low; acetic acid acted as an inhibitor, while ammonium hydrate and ammonium sulfate acted as activators. NaF showed little influence.
Autolysis phenomena due to addition of soybean oil during 48 to 72 hours of fermentation were parallel to higher lipase activity.View full abstractDownload PDF (487K) -
Part 4. Studies on Counter-measures against Toxicity of Soybean oilShuntaro YASUDA, Keizo YAMASAKI, Sachiko MIZOGUCHI1951 Volume 25 Issue 7 Pages 361-364
Published: 1951
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSIn bean the LC (lactose 3 %) medium, we could prevent the autolysis produced by oil and increase penicillin potency by the addition of soybean oil and inncrease penicillin potency by the following treatments:
0.1% of acetic acid (pH 6.0, K-salts) was added before the addition of soybean oil.
2. 0.1 to 0.05% of boric acid was added.
3.The initial pH was adjusted to 4.5 to 5.0.View full abstractDownload PDF (287K) -
XVI. Mitteilung. Enzyme der MausKazuo YAMAFUJI, Chinami TANAKA1951 Volume 25 Issue 7 Pages 364-366
Published: 1951
Released on J-STAGE: November 21, 2008
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XVII. Mitteilung. Herstellung von Alkohol aus Lycoris radiataKazuo YAMAFUJI, Toshihiko AKITA1951 Volume 25 Issue 7 Pages 366-367
Published: 1951
Released on J-STAGE: November 21, 2008
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Part 1. Synthesis of Peptide Bond by ProteaseKensuke SHIMURA, Kyohei YAMASHITA, Midori MASUYAMA1951 Volume 25 Issue 7 Pages 367-371
Published: 1951
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSEnzymatic synthesis of anilides of various carboxylic acids has been studied. As the result it has been found that the carboxylic acid which was concerned in formation of peptide bond should satisfy the general structure, R1•CONH-CHR2•COOH. When the hydrogen atom of NH or CHR group was substituted with an alkyl group, the synthesis of anilide was completely retarded.View full abstractDownload PDF (461K) -
Kisaku YAMADA, Taneko SUZUKI1951 Volume 25 Issue 7 Pages 372-376
Published: 1951
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSMalonic decarboxylas2 was prepared from the water extract of the skeletal muscle of fish by precipitating with acetone or by adsorbing on Ca8(PO4)2 and its activiyt was determined.
It was different according to the tissue and especially to the season.View full abstractDownload PDF (473K) -
Part 12. Conversion of glucose-1-phosphate into glucose-6-phosphate by broad bean phosphoglucomutaseKonoshin ONODERA1951 Volume 25 Issue 7 Pages 377-380
Published: 1951
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESS1. Various methods have been investigated for preparing the enzyme phosphoglucomutass which converts glucose-l-phosphote into glucose-6-phosphate from broad bean seeds (Vicia faba L.). It has been found to be unsuitable to fractionate the extract of seeds with ammonium sulfate.
2. The enzyme has been obtained free from other enzymes by adjusting the extract of broad bean seeds to pH 5. 0, follwed by heating at 50°C for 10 minutes.
3. The phosphoglucomutase prepared as above converts 94% of glucose-l-phosphate into glucose-6-phosphate at 30°C and pH 7.8.
4. Glucose-6-phosphate has been prepared in a pure state (as barium salt) by the action of the enzyme thus obtained on glucose-l-phophate.View full abstractDownload PDF (380K) -
Part 1. A Study of the Bacteriostatic Action for the Molds of Tobacco LeavesYuji SASAKI, Satohiko HATTORI1951 Volume 25 Issue 7 Pages 381-382
Published: 1951
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSThe authors studied the bacteriostatic activities of vapors of chemical reagents; alcohols, acids, aldehyds, esters, and others, for the molds of tobacco leaves.
Among these reagents, amyl alcohol, benzoic acid, valeraldehyde, vanillin, furfural, citronella and heliotropin showed relatively high activities, and furfural the highest.View full abstractDownload PDF (165K) -
Part 2. A Study of the Bacteriostatic Action of Furfural Vapor for MicroorgamismsYuji SASAKI, Satohiko HATTORI1951 Volume 25 Issue 7 Pages 383-386
Published: 1951
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESS1. The authors studied the bacteriostatic action of furfural vapor for microorganisms.
2. Furfural vopor showed high bacteriostatic action for the development of fungi, but was relatively weak for yeasts and bacteria.
3. For the development of Aspergillus sp. (19 strains) and Penicillium sp. (13 strains) fur-fural vapor showed high bacteriostatic action, but several strains of Penicillium sp. have some resistance against the vapor.
4. Furfural and its derivatives; furan, furfuryl alcohol, furan carboxylic acid, furfural acetone, furfural oxime, furfuracrolein, furfuryl acetate, and furfuracrylic acid are used also in the expeximents. The results of the experiments showed that furfural and furan had highest bacteriostatic properties for the development of Asp. oryzae. However we noted that in this case these derivatives had no bactericidal power.View full abstractDownload PDF (339K) -
Part 7. On the Abnomal Waves of 2, 6-dichlorophenolindophenolIsamu TACHI, Tsutomu TSUKAMOTO1951 Volume 25 Issue 7 Pages 386-391
Published: 1951
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSIn strongly alkaline medium 2, 6-dichlorophenolindophenol gives anomalous three waves on the polarogram and the current intensity of the limiting diffusion current (wave-heigth) changes with the time of standing. Their reduction potentials were -0.345 V, -0.555 V, and -0.948 V (vs. N.C. E.) respectively.
From the polarograms obtained at pH 14, the first and the second waves are considered most probably as the waves due to the inclusion of semiquinone and the last wave must be due to the reduction of the decomposition product.
The structure of semiquinone seems to be as follows ; and it is anionic sensiquinons that is stable.
According to the Michaelis' theory the semiquinone form-ation constant is about 20 in a buffered solution of pH 12.2. But we are of opinion that the semiquinone is formed not only by the electrolytic reduction but by the inner reaction of the solution itself upon standing.
_??_View full abstractDownload PDF (418K) -
Part 6. On the Carboxyl Groxyl Group of Wood Polyuronide HemicelluloseI. TACHI, N. YAMAMORI1951 Volume 25 Issue 7 Pages 392-397
Published: 1951
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSIn the present studies on the samples of polyuronide hemicellulose prepared from elm, beech, and cherry woods, comparative estimations of the carboxyl groups by the carbon dioxide evolution, calcium acetate and potentiometric titration methods were carried out in order to ascertain whether the carboxyl groups in the molecules are free or bound, and simultaneously, whether the caictium acetate or potentiometric titration methods is applicable in estimating carboxyl groups quantitatively.
The results are summerized as follows:
(1) The acid decomposition with 12% HCI for 5 hours makes the evolution of carbon dioxids completely quantitative from glucuronic acid residus of the sample.
(2 ) Gocd agreement is obtained between the carboxyl values found by the potentiometric titra-tion in presence of NaBr and by the calcium acetate method for every sample, but these values are smaller than the theoretical values calculated from the quantiites of carbon dioxide evolved by acid decomposition.
(3) From this difference, it would seem reasonable to assume that all carbovyl groups in the pclyuronide hemicellulose are not free, and that a limited amount of bound or potential carboxyl, at least, may be in the form of intermolecular anhydride or ester groups or as intramoleculai lactone.View full abstractDownload PDF (603K) -
Part 1. Thiocyan Radical. (1)Nobu ITO, Kazuo MORI, Minoru WATANABE1951 Volume 25 Issue 7 Pages 397-399
Published: 1951
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSThe germistatic and germicida activities of thiocynoacetic acid ethyl ester, used as a food preservative, and other 6 kinds of thiocyano esters, and 7 kinds of acetic acid esters were observed.
The thiocyano radical shows no particular germicidal actio, but fairly inhibits the growth of microbes.View full abstractDownload PDF (260K) -
Preparation of Aryl para-amino-εalicylatesSuttekiti MAKUYAMA, Hisashi IMAMURA1951 Volume 25 Issue 7 Pages 400
Published: 1951
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSDownload PDF (98K)
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