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Volume 52, Issue 1
Displaying 1-16 of 16 articles from this issue
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Sanetaka SHIRAHATA, Hiroki MURAKAMI, Koji YAMADA, Tamiyoshi SONDA, Kaz ...1978 Volume 52 Issue 1 Pages 1-9
Published: 1978
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSEffects of nucleic acid components on the Cu2+-catalyzed oxidation of adrenaline, which was one of aromatic reductones, was investigated by ESR analyses.
Adrenaline-Cu2+ mixture exhibited an ESR spectrum having main 3 signals; a, b and c, which were derived from adrenaline-Cu2+ complex. This spectrum was stable in the presence of oxygen, but the intensity of these signals was gradually decreased in the solution saturated with N2 gas. Especially, that of the signal b reflected the tendency strongly.
5'-AMP and cytosine which were known to promote the oxidation of aromatic reductones, accelerated the formation of adrenaline-Cu2+ complex. Addition of NADH to the adrenaline-Cu2+ mixture caused a remarkable decrease of the intensity of ESR signals even in the presence of oxygen as well as in the N2 gas saturated solution. This was interpreted by the bifunctional effects of NADH.
ESR spectrum of adrenaline-Cu2+ mixture was altered by the addition of ADP or ATP which suppressed the oxidation of adrenaline.View full abstractDownload PDF (618K) -
Jin-Ha LEE, Kazuki SHINOHARA, Hiroki MURAKAMI, Hirohisa OMURA1978 Volume 52 Issue 1 Pages 11-17
Published: 1978
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSThe reaction of triose reductone (TR) with nucleic acid bases, in 0.1N HCl at 100°C, gave rise to an intense browning with purine bases such as Ade, purine and Gua, but it did not with other purines as caffeine, theophylline or theobromine, indicating that nitrogen atoms and amino groups in the purine bases might be involved in the browning reactions. No additional browning occurred in the similar reactions of TR with pyrimidine bases such as Cyt, Thy or Ura to that caused with TR alone.
Similar tendencies in the browning were also observed in the reactions of TR with nucleosides or nucleotides, that is, Ado, Ado-5'-P or Guo-3'-P, gave intense browning, while Ino or Ino-5'-P showed only a weak browning.
On the other hand, the reducing power increased in an early stage of the reactions of TR with nucleic acid related compounds, showing the formation of the products possessing the reducing activity. This was confirmed from the results of paper chromatography and electrophoresis, especially in the reaction of TR with Ade, Gua, Cyt, Ado, Guo, Cyd, Ino, Ado-5'-P, Guo-3'-P, Guo-5'-P, Cyd-5'-P or Ino-5'-P.View full abstractDownload PDF (516K) -
Kazuo INA, Kumi HIGASHI1978 Volume 52 Issue 1 Pages 19-23
Published: 1978
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSAs a preliminary study to identify feeding stimulants for the sea bream, Chrysophrys major, aqueous methanolic extracts of many kinds of aquatic organisms were fractionated into acetoneinsoluble (B), acetone- and ether-soluble (C), and acetone- and water-soluble (A) fractions, and their stimulatory potencies were assayed. Results obtained are summarized as follow.
(1) In most of the aquatic animals examined, the strongest activities were found in Fr. B.
(2) Among various algae tested, only Fr. A of two species, Endarachne binghamiae and Porphyra suborbiculate, was stimulative, but its activity was weader than that of Fr. B of animal origin.
(3) In viscera of the squid, Todarodes pacificus and whole body of the mysid shrimp, Anisomysis ijimai, all of the three fractions induced active feeding behaviors.View full abstractDownload PDF (213K) -
Shigeaki TAKAGI1978 Volume 52 Issue 1 Pages 25-30
Published: 1978
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSAn interaction between protein and carotenoid was investigated. When ethanol solution of lutein was added to aqueous solution of azuki protein, a combined product which might be considered to a complex between protein and lutein was produced in the form of solution or precipitation. In this case lutein was completely combined to protein till the quantitative ratio of lutein to protein was reached to 8.8×10-3. pH effected to the formation and the precipitation of the complex. At an isoelectric point heterogeneous precipitants were produced, which were composed of both colored complex and colorless subjected protein. Above this pH homogeneous complex was produced, the molecular weight of which was estimated to be 507, 000 by gel filtration and was not varied by the quantities of added lutein. The complex formation was not appreciably effected by the ionic concentration, and the precipitation of complex easily occured at higher ionic concentration than 0.05M.
Nonionic detergents accerated the formation of complexes while anionic detergents inhibited. The complex formation was strongly inhibited by dissociation agents, both urea and guanidine.
Proteins other than Azuki protein, that is, casein, ovalbumin and soy 11S protein, were also similarly combined to lutein to produce complexes.
From these results it was considered that lutein combined to the hydrophobic parts on the protein surface followed by association to produce complex.View full abstractDownload PDF (465K) -
Hideo OCHIAI, Hitoshi SHIBATA, Tetsu MATSUO, Kenji HASHINOKUCHI, Masao ...1978 Volume 52 Issue 1 Pages 31-36
Published: 1978
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSChloroplast electron flow system was successfully immobilized inside polyacrylamide gel formed by the redox polymerization. The immobilization system consisted of acrylamide monomer (15%, final concentration), N, N'-methylene-bisacrylamide (0.8%), ammonium persulfate (0.02%), ascorbate (0.06%), bovine serum albumin (1%), D-mannitol (0.01%) and the broken chloroplasts, type C, in 0.05M Tris buffer Tris buffer containing 0.4M sucrose and 0.01M NaCI (STN buffer). Incubation of the reaction mixture at 10°C for 2 hr yielded the gel keeping Photosystem I activity as 11.3% of the native chloroplasts (control) and Photosystem II activity as 11.2% of the control. The immobilized chloroplast gel retained activities of Photosystem I and Photosystem II even after being allowed to stand for several weeks in the STN buffer at 4°C. The optimum pHs of the immobilized Photosystem I and Photosystem II were the same as those of the untreated chloroplasts: pH 8.6 for Photosystem I and pH 6.2 for Photosystem II. However, the Photosystem II activity of the gel was completely lost in 2 days on standing above pH 8. The thermo-stabilization of Photosystem I and Photosystem II was also attained by the immobilization. Under anaerobic condition, electric current was observed on illumination onto the chloroplast photo-cell system which was composed of the gel, methyl viologen as an electron acceptor, glucose plus glucose oxidase as an oxygen dissipating system and sodium azide in the STN buffer.View full abstractDownload PDF (519K) -
Teruo MIYAZAWA, Yasuhiko FUJINO1978 Volume 52 Issue 1 Pages 37-43
Published: 1978
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESS1. Glyceroglycolipids, monoglycosyldiglyceride (MGD), diglycosyldiglyceride (DGD), triglycosyldiglyceride (TGD) and sulfoquinovosyldiglyceride (SQD) were isolated from rice bran and characterized for their chemical constituents and molecular species.
2. Galactose and glucose were found as component sugars at the ratios of 92:8, 85:15 and 63:37 for MGD, DGD and TGD, respectively.
3. The major component fatty acids were palmitic, oleic, linoleic and linolenic acids. Linoleic acid was predominant in MGD, DGD and TGD, while palmitic acid was in SQD.
4. In rice bran glyceroglycolipids, unsaturated fatty acids were mostly located in C-2 position for MGD and DGD, whereas in C-1 position for SQD.
5. Twelve different diglyceridic molecular species were detected in MGD, DGD and SQD, respectively. Among them, the representative species were 1-18:2-2-18:2, 1-16:0-2-18:2 and 1-16:0-2-18:3 for MGD and DGD, and 1-16:0-2-16:0, 1-18:2-2-16:0 and 1-18:1-2-16:0 for SQD.View full abstractDownload PDF (470K) -
Jun-ichi IWAMURA, Nenokichi HIRAO1978 Volume 52 Issue 1 Pages 45-48
Published: 1978
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSAn essential oil was obtained from the fresh herb of Vilex rotundifolia L. fil. by steam distillation in 0.033_??_0.066% yield.
The components were separated by means of column and gas chromatography, and as major components, camphene, β-pinene, limonene, linalool, terpinen-4-ol, α-terpinyl acetate, and dehydroabietane, and as minor components, β-myrcene, bornyl acetate, δ-elemene, α-copaene, α-muurolene, δ-cadinene, β-guaiene, and α-cadinol were identified in terms of tR in GC and MS spectra.
Moreover, the essential oils obtained individually from flower, leaf, and stalk were analyzed, the principal constituents of the essential oil of flower were camphene and diterpenes, ones of leaf were α-terpinyl acetate and diterpenes, and ones of stalk were β-pinene, 1, 8-cineole and diterpenes.View full abstractDownload PDF (255K) -
Hisao TSUJI, Yumi TANI, Hiroo UEDA1978 Volume 52 Issue 1 Pages 49-50
Published: 1978
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSThe roots of Solidago altissima L. were extracted with hot methanol. The essential oil was removed from the methanol extract by steam distillation. The residue was extracted with ethyl acetate followed with ether.
The neutral fraction of the ether extract was chromatographed on an alumina column to give two crystalline compounds, which were identified as β-amyrin and α-spinasterol.
A crystalline compound, which was obtained from the ethylacetate extract by means of chromatography on a silica gel column, was identified as α-spinasteryl glucoside.View full abstractDownload PDF (165K) -
Ryo YAMAUCHI, Setsuro MATSUSHITA1978 Volume 52 Issue 1 Pages 51-52
Published: 1978
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSFor the fractional determination of tocopherols, a combined method of thin-layer chromatography (TLC) and densitometry was tested. Samples of oils were saponified in the presence of pyrogallole, and tocopherols in the unsaponifiable matter were separated by TLC. The tocopherols on the TLC plate were determined by measuring the optical density at 295nm by a densitometer. This method was simple and quantitative for determining tocopherol contents of vegetable oils.View full abstractDownload PDF (152K) -
1978 Volume 52 Issue 1 Pages N16
Published: 1978
Released on J-STAGE: November 21, 2008
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A Novel Pathway of Metabolism of PropionateTakeshi TABUCHI1978 Volume 52 Issue 1 Pages R1-R8
Published: 1978
Released on J-STAGE: November 21, 2008
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[in Japanese]1978 Volume 52 Issue 1 Pages N1a-N2
Published: 1978
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSDownload PDF (199K) -
[in Japanese]1978 Volume 52 Issue 1 Pages N1
Published: 1978
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSDownload PDF (100K) -
J. M.1978 Volume 52 Issue 1 Pages N2-N3
Published: 1978
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSDownload PDF (216K) -
[in Japanese]1978 Volume 52 Issue 1 Pages N3-N4
Published: 1978
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSDownload PDF (216K) -
N. Y.1978 Volume 52 Issue 1 Pages N4-N5
Published: 1978
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSDownload PDF (185K)
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