All issues
Volume 48, Issue 5
Displaying 1-10 of 10 articles from this issue
- |<
- <
- 1
- >
- >|
-
Norikazu MATSUMOTO, Tadao HASEGAWA, Takao SUZUKI1974 Volume 48 Issue 5 Pages 279-284
Published: 1974
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSIn the preceding papers, we proposed the following explanation: L-cysteine formed from L-methionine and L-serine through cystathionine pathway in the skin tissues, and L-methionine and L-serine exhibited the effectiveness to the hair growth. However L-methionine has not the practical value because of the illsmelling compound. Whereas acetyl-L-methionine was recognized as the odorless compound by the preservation test, acetyl-L-methionine was used for experiments of L-cysteine formation instead of L-meth-ionine. In addition, another amino acids, peptide and nucleoside were applied to the same purpose. The test of hair growth were made by applying the amino acids solution on the rabbit skin, that is, 0.2% L-methionine, L-serine, 0.2% acetyl-L-methionine, L-serine, 0.2% glutathione, 0.2% L-cysteine, 0.2% L-methionine, L-serine and inosine were made up as the amino acids solution respectively. Results showed, L-methionine, L-serine solution was very efficacious, and acetyl-L- methionine, L-serine solution L-cys-teine solution, L-methionine, L-serine and inosine solution were also efficacious, but glutathione solution showed the inhibitory actions to both of the growth of hair and hair roots.View full abstractDownload PDF (2161K) -
Hiroshi HAGINO, Hiroshi KASE, Kiyoshi NAKAYAMA1974 Volume 48 Issue 5 Pages 285-290
Published: 1974
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSAn L-lysine auxotrophic mutant of Corynebacterium glutamicum M-14 was found to produce L-isoleucine at a low concentration of L-lysine. The optimum concentration of L-lysine for L-isoleucine accumulation was 10_??_20μg/ml and 2.0mg/ml of L-isoleucine was produced in a synthetic medium containing glucose (10%) as a carbon source. L-Homoserine and L-aspartic acid increased L-isoleucine production in the medium containing L-lysine at the concentration (100μg/ml as monohydrochloride) inhibitory to de novo production of L-isoleucine. Addition of L-methionine or L-threonine decreased L-isoleucine production remarkably in the medium containing L-lysine at the concentration optimal for de novo production of L-isoleucine (10μg/ml as monohydrochloride). α-Aminobutyric acid and α-hydroxybutyric acid increased the L-isoleucine production significantly and 6.2mg/ml of L-isoleucine was produced when 10mg/ml of α-hydroxybutyric acid was supplemented to the medium.
The effect of α-aminobutyric acid and a-hydroxybutyric acid on the increase of L-isoleucine production was noted also in some hydrocarbon utilizing microorganisms.View full abstractDownload PDF (502K) -
Ryuzo NISHINO, Yoshinori OZAWA, Atsushi YASUDA, Toshio SAKASAI1974 Volume 48 Issue 5 Pages 291-296
Published: 1974
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSAutomated analysis of oligosaccharides in soy sauce was performed according to gel filtration method with Biogel P-2 by using JLC-6 AH liquid chromatograph apparatus. As the results, mono-, di-, tri-, tetra-, and polysaccharides in soy sauce were separated.
The contents of oligosaccharides in Koikuchi and Usukuchi shoyu, almost of which consisted of disaccharides, were about 0.5g/100ml. On the other hand, the contents of oligosaccharides in Tamari and Shiro shoyu, almost of which consisted of di-, tri-, and tetrasaccharides, were about 1.0 and 3.0g/100ml, respectively.
In Koikuchi shoyu mash, there existed di-, tri-, and tetrasaccharides during first 20 days of aging. After stage of alcohol fermentation, the tri-, and tetrasaccharides almost disappeared from the mash.
Each fraction separated by gel filtration was chromatographed on anion exchange resin with borate buffer. As the results, several peaks were detected in each chromatographic pattern. The sugar composition of the di-, and trisaccharide fractions were determined as glucose, galactose, arabinose, xylose and mannose. Among them, glucose was main component. The tetrasaccharide fraction from Tamari shoyu was composed of considerably amount of rhamnose. In the case of Shiro shoyu, tetrasaccharide fraction consisted of a lot of xylose and arabinose.View full abstractDownload PDF (472K) -
Hiroo WADA1974 Volume 48 Issue 5 Pages 297-302
Published: 1974
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSScreening tests were carried out to obtain microorganisms capable of converting DL-2-hydroxy-4-methylthiobutyric acid (DL-HMBA) to L-methionine.
From the tests, it was shown that the microorganisms useful for the conversion were distributed over the wide range of genus. Among these, a bacterial strain, G-132-13, converted DL-HMBA to L-methionine in an excellent yield.
This strain is Gram-negative, moves with polar flagella, and reduces nitrate with production of nitrogen gas. From these taxonomic properties, this strain was classified as a strain of Pseudomonas denitrificans.View full abstractDownload PDF (390K) -
Hiroo WADA1974 Volume 48 Issue 5 Pages 303-311
Published: 1974
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSIn order to find out the proper conditions for L-methionine production from DL-2-hydroxy-4-methylthiobutyric acid (DL-HMBA) by fermentation with Pseudomonas denitrificans G-132-13, which was chosen in the previous paper as a suitable strain for the production, the effect of cultural conditions was studied.
As a result of the studies, this strain gave 89% of theoretical yield of L-methionine, 20.8mg/ml, in 49 hours submerged culture with following medium: DL-HMBA ammonium salt, 2.35% (as free acid); glucose, 5%; corn steep liquor, 2%; ammonium chloride, 0.25%; pH, 7.8.
The conversion of DL-HMBA to L-methionine was catalyzed with two enzyme systems, NAD, NADP-unlinked DL-HMBA dehydrogenase and transaminase.View full abstractDownload PDF (696K) -
Katsumi YUASA, Keiko ISHIZUKA, Susumu KABURAKI, Toshio SAKASAI1974 Volume 48 Issue 5 Pages 313-318
Published: 1974
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSIn previous paper, isolation and identification of catechol from shoyu was reported. In this paper, the formation of catechol was studied.
The results were as follows.
1) When Aspergillus soyae (KS) was cultured for 7 days at 30°C on Czapek solution containing 0.1% tryptophan, 2, 3-dihydroxybenzoic acid and catechol were formed from tryptophan.
2) 2, 3-Dihydroxybenzoic acid was isolated from Czapek solution containing 0.1% tryptophan cultured by Aspergillus soyae (KS).
3) Catechol was isolated from Czapek solution containing 0.1% 2, 3-dihydroxybenzoic acid cultured by Aspergillus soyae (KS).
4) Productivity of 2, 3-dihydroxybenzoic acid and catechol from tryptophan was found in strains of Aspergillus.
5) By resting cell culture of Aspergillus soyae (KS) in 1/15M phosphate buffer (pH 5.5), 2, 3-dihydroxybenzoic acid and catechol were formed from tryptophan, kynurenine, and anthranilic acid but not formed from kynurenic acid, 3-hydroxyanthranilic acid and o-aminophenol.
6) From these results, we recognized the pathway for the formation of catechol in shoyu via kynurenine, anthranilic acid and 2, 3-dihydroxybenzoic acid from tryptophan in Aspergillus.View full abstractDownload PDF (437K) -
Seisuke ITO, Yasuhiko FUJINO1974 Volume 48 Issue 5 Pages 319-323
Published: 1974
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESS1. Alfalfa leaves contained at least eleven kinds of phospholipids among which seven were identified, namely, phosphatidylcholine, phosphatidylethanolamine, lysophosphatidylcholine, lysophosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol and phosphatidylserine.
2. Main fatty acids in phosphatidylcholine and phosphatidylethanolamine were linoleic, palmitic and linolenic acids, whereas those in phosphatidylglycerol and phosphatidylinositol were palmitic, palmitoleic, linolenic and linoleic acids.
3. Saturated fatty acids were mainly distributed in α positions and unsaturated ones predominantly occupied β positions both in phosphatidylcholine and phosphatidylethanolamine.
4. Natural lysophosphatidylcholine and lysophosphatidylethanolamine were found to be acylated in a and β positions, respectively.View full abstractDownload PDF (310K) -
Saburo TAKANO, Tadao HASEGAWA, Hideo MUKO, Takao SUZUKI1974 Volume 48 Issue 5 Pages 325-328
Published: 1974
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSRecently amino acid derivatives have received attention as efficacious pesticides against the plant pathogen.
In this report, several amino acid derivatives were tested for their antibiotic effects upon the plant pathogen. Following amino acid derivatives were synthesized: N-benzoyl-L-valine, N-benzoyl-L-leucine, N2, N6-dibenzoyl-L-lysine, N-benzoyl-L-isoleucine, N-phenyl-acetyl-L-valine, N-phenylacetyl-L-leucine, N2, N6 -diphenylacetyl-L-lysine, N-phenylacetyl-L-isoleucine.
Then the screening tests of these compounds were carried out with sheath blight of rice plant, anthracnose of banana, etem rot of banana, black spot of pear, gray mold of cucumber.
Results showed N-benzoyl-L-leucine caused a significant inhibition in concentration of 0.1mM to the colony appearance of anthracnose of banana and black spot of pear respec-tively.
Other derivatives have some antibiotic activities to the plant pathogen, but these might be of no practical value because of the weak activities.View full abstractDownload PDF (260K) -
1974 Volume 48 Issue 5 Pages N66
Published: 1974
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSDownload PDF (79K) -
1974 Volume 48 Issue 5 Pages e1
Published: 1974
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSDownload PDF (72K)
- |<
- <
- 1
- >
- >|