The hydrol from the enzymatic hydrolysate of starch was analysed. The contents of total sugar and reducing sugar were 90.21% and 70.08%, respectively. The content of moisture was 8. 84% and ash 0. 11%.
By chromatographic methods, 12 spots corresponding to glucose, fructose, nigerose, maltose, isomaltose, laminaribiose, gentiobiose, maltotriose, panose, isopanose, isomaltotriose and an unidentified sugar were detected. The main sugar was glucose and its content was about 55% of the total sugar, and the contents of maltose fraction and isomaltose fraction were 10.17% and 7.21% of the total sugar.
Hydrol from the enzymatic hydrolysate of starch was fractionated by a carbon-Celite column using water and 2.5_??_20% ethanol as successive elution solvents. On acetylation of isomaltose fraction, isomaltose was identified as crystalline octaacetate. Nigerose, maltose and gentiobiose were also isolated by successive carbon-Celite and Magnesol-Celite column chromatography, and identified as their crystalline octaacetates. Laminaribiose was isolated by thick paper chromatography and was also identified as its crystalline octaacetate. Panose was identified as crystalline panitol dodecaacetate.
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