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Mitsu Kayama
2001 Volume 67 Issue 6 Pages
1039-1050
Published: November 15, 2001
Released on J-STAGE: February 01, 2008
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At the beginning of the new century, it is very important to review studies on marine biogenic lipids conducted in the last century. Initially, studies on marine lipids concentrated on finding new lipids and their structures; there were few biochemical studies on fish and other aquatic organisms in the field of lipid metabolism until the later stage. Advanced methods such as gas-liquid chromatography for fatty acid analysis and radioisotope tracer technique have permitted such studies on aquatic organisms. In order to investigate lipid metabolism in aquatic life, the authors studied the biogenesis of highly unsaturated fatty acids, especially eicosapentaenoic and docosahexaenoic acids, and the conversion to eicosanoids in fish. The metabolism of other marine lipid classes, such as squalene, glyceryl ethers and wax esters in oceanic world was also studied.
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Koji Takeno, Mamoru Yoshiya, Toshiaki Miyajima
2001 Volume 67 Issue 6 Pages
1051-1055
Published: November 15, 2001
Released on J-STAGE: February 01, 2008
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Meristic characteristics were studied in Japanese flounder Paralichthys olivaceus collected in western Wakasa Bay with the focus on two distinct populations. The numbers of dorsal and anal fin rays were compared between immature and adult fish collected from 1994 to 2000. Matured adult fish collected in the spawning season had significantly lower number of dorsal and anal fin rays compared to the immature fish collected in the same area. Considering the previous reports which showed that there were two distinct populations as resident (lower fin ray number) and migrant (higher fin ray number), we speculated that the higher fin ray number population emigrates westward from western Wakasa Bay at one or two years old, whereas the lower fin ray number population stays in this area throughout its life.
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Akihiro Okamura, Huan Zhang, Yoshiaki Yamada, Tomoko Utoh, Naomi Mikaw ...
2001 Volume 67 Issue 6 Pages
1056-1060
Published: November 15, 2001
Released on J-STAGE: February 01, 2008
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Discriminant functions as a simple method for identifying two eel species, Anguilla japonica and A. anguilla, were developed using four morphometrical characteristics. In order to test the validity of the functions, species discrimination using the functions was carried out on a total of 162 eels obtained from four natural waters (a river in Kannonji City and Lakes Shinji, Jinzai and Ikeda), and the result was compared with that of identification by PCR-RFLP analysis. This analysis showed that discrimination using the functions was more than 90% correct, indicating that this was an effective identification tool. The species discrimination showed that the rates of occurrence of A. anguilla in each area were 10% for the river in Kannonji City, 15.1% for Lake Shinji, 20% for Lake Jinzai and 93.5% for Lake Ikeda.
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Koji Takeno, Mamoru Yoshiya, Toshiaki Miyajima
2001 Volume 67 Issue 6 Pages
1061-1064
Published: November 15, 2001
Released on J-STAGE: February 01, 2008
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In order to clarify the stock structure of the Japanese flounder Paralichthys olivaceus in western Wakasa Bay, the first annual ring radius of the otolith was compared between immature and adult fish collected from 1994 to 2000. The average radius of the first ring in adult fish was significantly smaller than that of the same year-class immature fish. This phenomenon was observed in both females and males. Considering the previous reports which showed that there were two distinct populations as early settlers and late settlers, we speculated that the early settlers with larger fin ray number grow fast and migrate westward at one or two years old, whereas the late settlers with smaller fin ray number grow slowly and stay in this area throughout their life.
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Yukihiko Serisawa, Hiroyuki Takaki, Akira Kurashima, Yasutsugu Yokoham ...
2001 Volume 67 Issue 6 Pages
1065-1071
Published: November 15, 2001
Released on J-STAGE: February 01, 2008
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This study was designed to examine the relationships between the seasonal fluctuations in nitrate concentration in seawater and photosynthetic activity of the bladelets of Ecklonia cava. Dissolved inorganic nitrogen (DIN) and nitrate concentrations in seawater and the net photosynthetic rates of E. cava were measured every month in Nabeta Bay, Shimoda, Izu peninsula, Japan. The concentrations of DIN and nitrate and the photosynthetic activities of E. cava showed the same seasonal change, high in winter and low in summer. The net photosynthetic rates of bladelets of E. cava were also measured every two months after incubation for two days in the nitrate enriched seawater and in fresh seawater. The maximum photosynthetic rates of bladelets of E. cava were slightly higher in the nitrate enriched seawater than in the fresh seawater in each month, but this difference was not significant by the t-test.
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Nozomu Daida, Hiroshi Inada, Tomohiro Miki, Takuhiro Sakai, Haruyuki K ...
2001 Volume 67 Issue 6 Pages
1072-1081
Published: November 15, 2001
Released on J-STAGE: February 01, 2008
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Flume tank experiments were conducted to clarify the relationship among tied-eye position, line tension and diving behavior of crank bait lures with different shapes of lip. Experimental lure-lips having the same area and different aspect ratio (λ=0.3, 0.5, 1.0, 1.5, 2.0) in the plane view were made. Trial lures with the same body were used in the experiments at velocities of 40, 60 and 80cm/s in the flow. Attack angle of the lures and tension on the towing line were measured in the tank test. The lure behavior was also recorded by video cameras from the top and the side of the observation area. We used an index C
S (=S
F/S) that indicates the areal ratio of the forepart of the tied-eye (S
F) to the whole area (S) of the lure in the plane view. It was confirmed that there was a constant relationship respectively between the areal ratio (C
S) and line tension on a towing line, C
S and attack angle of the line, even for different shapes of lip attached. In the case of different lips, the tested lures showed steady diving behavior within the range of C
S=0.2 to 0.3.
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Atsushi Yamasaki, Shigeru Ohki, Satoshi Iizuka
2001 Volume 67 Issue 6 Pages
1082-1088
Published: November 15, 2001
Released on J-STAGE: February 01, 2008
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This paper presents the mesh selectivity for willowy flounder Tanakius kitaharai using a beam trawl net with experimental bags of three different mesh opening : 35.8mm, 42.9mm and 49.4mm. Forty seven hauls of beam trawl towing were carried out in the area between 100 and 180m in depth off Kyoto Prefecture from August 1999 to August 2000. The parameters of logistic and Richards equations for mesh selectivity curves in each mesh opening were estimated by the maximum likelihood method. The Richards equation model was selected as the betterfit model because it had a smaller value of AIC than the logistic equation model. The values of 50% selection length of 35.8mm, 42.9mm and 49.4mm sizes were estimated to be 97.0mm, 112.4mm and 129.6mm, respectively. On the other hand, many juveniles of willowy flounder were clearly caught by commercial fishery with codend of 20.9mm mesh opening. This paper also discusses the effectiveness of mesh enlargement for protecting juvenile flounders based on mesh selectivity estimated in this study.
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Yuko Ito, Ikuo Hayashi
2001 Volume 67 Issue 6 Pages
1089-1095
Published: November 15, 2001
Released on J-STAGE: February 01, 2008
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Diel movements were observed and compared for three herbivorous gastropods, Turbo cornutus, Omphalius pfeifferi carpenteri and Astralium haematragum, which dominate the shallow rocky coasts of the Japan Sea. Experiments were conducted under 12L-12D conditions using a very high sensitivity ICCD television camera. T. cornutus was more active in dark than light conditions, and moved at a speed of 8-11cm/min. The distance moved and time spent moving per 24 hours were 37-39m and 7 hours, respectively. O. pfeifferi carpenteri also showed a more active habit in dark conditions, and moved at a speed of 2-5cm/min with the distance of 5-13m and time of 3-6 hours per 24 hours. In contrast to the two species, A. haematragum did not show any diurnal rhythm. It moved at a rather slow speed of 1-2cm/min with the distance of 0.8-2.6m and time of 1.5-4 hours per 24 hours. The behavioral significance of these three species was discussed, particularly in connection with predators.
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Akiko Taniguchi, Katsumi Takano
2001 Volume 67 Issue 6 Pages
1096-1102
Published: November 15, 2001
Released on J-STAGE: February 01, 2008
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Carboxypeptidases of the intestine of Tilapia nilotica were purified by ammonium sulfate precipitation, followed by trypsin inhibitor-sepharose 4B affinity chromatography, DEAE-and CM-cellulose ion-exchange chromatographies, and polyexchanger PBE 94 chromatofocusing. Three purified carboxypeptidases were tentatively designated carboxypeptidase I, II and III. Each of the three carboxypeptidases was found to be a single band when examined by poly-acrylamide gel electrophoresis. The purifications of carboxypeptidase I, II and III were 63-, 77-and 79-fold from the crude extract, respectively. The carboxypeptidases showed the optimum activity at pH8.0 or 8.5 and 30°C, and carboxypeptidase I was specifically found to be able to hydrolyze Bz-Gly-Tyr, while II and III were found to be able to hydrolyze Bz-Gly-Arg. Three enzymes were strongly inhibited by o-phenanthroline. The activities of three enzymes inactivated by o-phenanthroline were regenerated completely by the addition of Co
2+, Ni
2+ and Zn
2+.
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Nobuhiko Dohmoto, Keng Chih Wang, Tetsu Mori, Ikuo Kimura, Tsuyoshi Ko ...
2001 Volume 67 Issue 6 Pages
1103-1109
Published: November 15, 2001
Released on J-STAGE: February 01, 2008
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A new type of fish sauce was prepared from two kinds of fish, Macruronus novaezelandiae and Thunnus maccoyi, and a squid Loligo bleekeri employing koji-mold, lactobacilli, and yeast which were adopted in soy sauce fermentation. During and after fermentation, chemical analyses were performed to determine pH, total nitrogen, reducing sugar, alcohol, and free amino acids. The sauces fermented for 6 months had a pH ranging from 4.8 to 5.0 which was the same as that of usual soy sauce. Total nitrogen content, however, ranged from 1.78 to 1.86% which was similar to that of traditional fish sauces (nuoc-mam). In terms of free amino acids, glutamic acid, alanine, leucine, and lysine increased during fermentation and were abundant in fish sauces. Sensory evaluation analysis proved that these fish sauces had a mellow taste and savory aroma without unpleasant smell in comparison with the traditional fish sauces.
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Yasuhiro Funatsu, Kenichi Kawasaki, Shiro Konagaya
2001 Volume 67 Issue 6 Pages
1110-1119
Published: November 15, 2001
Released on J-STAGE: February 01, 2008
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A fish sauce (FMS) prepared from gutted frigate mackerel on a test plant scale was compared in the volatile compounds among some fish sauces such as (Shottsuru (S), Ishiru made from sardine (IS), and a sauce from Japanese common squid (IJCS)), and soy sauce (SS) which are common in Japan. In FMS and SS, a few volatile acids (VA) and many kinds of alcohols were found. In S and IS, a variety of VA including butanoic and pentanoic acids were detected. On the other hand, in IJCS only acetic acid was detected as VA, but many kinds of aldehydes were found. There were no butanoic and pentanoic acids in FMS, SS and IJCS. By sensory evaluation, the flavor factors of FMS and SS were judged to be agreeable and to form a moderate flavor, and those of S, IS and IJCS were judged to be slightly disagreeable and irritating. Moreover, a strong positive correlation was observed between the total amount of VA and pH of the sauces, while a strong negative correlation was found between the agreeability of flavor and pH among all the samples excluding IJCS.
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Yuji Hotta, Masato Aritaki, Masatomo Tagawa, Masaru Tanaka
2001 Volume 67 Issue 6 Pages
1120-1121
Published: November 15, 2001
Released on J-STAGE: February 01, 2008
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Shigehisa Yamasaki, Arata Kawaida
2001 Volume 67 Issue 6 Pages
1122-1123
Published: November 15, 2001
Released on J-STAGE: February 01, 2008
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Hiromu Zenitani
2001 Volume 67 Issue 6 Pages
1125-1126
Published: November 15, 2001
Released on J-STAGE: February 01, 2008
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Chifumi Imai
2001 Volume 67 Issue 6 Pages
1127-1128
Published: November 15, 2001
Released on J-STAGE: February 01, 2008
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Tetsuichiro Funamoto
2001 Volume 67 Issue 6 Pages
1129-1130
Published: November 15, 2001
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Isamu Mitani
2001 Volume 67 Issue 6 Pages
1131-1132
Published: November 15, 2001
Released on J-STAGE: February 01, 2008
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Yoshinari Tsuruta
2001 Volume 67 Issue 6 Pages
1133-1134
Published: November 15, 2001
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Akinori Hino
2001 Volume 67 Issue 6 Pages
1136-1137
Published: November 15, 2001
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Kenji Yoshimura
2001 Volume 67 Issue 6 Pages
1138-1139
Published: November 15, 2001
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Hiroshi Kuwada
2001 Volume 67 Issue 6 Pages
1140-1141
Published: November 15, 2001
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Kenji Tanaka
2001 Volume 67 Issue 6 Pages
1142-1143
Published: November 15, 2001
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Takao Yoshimatsu
2001 Volume 67 Issue 6 Pages
1144-1145
Published: November 15, 2001
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Masahiro Hayashi
2001 Volume 67 Issue 6 Pages
1146-1147
Published: November 15, 2001
Released on J-STAGE: February 01, 2008
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